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Biomedical subjects

W L Gyure

Publications and source records attributed to W L Gyure.

4 recordsLinked to original sources
PubMed1992

The properties of monoclonal antibody against sepiapterin reductase from fat body of the silkworm, Bombyx mori.

A specific monoclonal antibody prepared for the 29-kDa a subunit of silkworm fat body sepiapterin reductase (SPR) was able to recognize the subunit in crude extract of fat body after SDS treatment. Although SPR from the silkworm fat body has biochemical properties similar to those reported for SPR from mammalian sources, especially rat erythrocytes, the antibody failed to recognize the 28-kDa subunit of rat erythrocyte SPR. This result indicates that SPR from silkworm fat body has a different amino-acid sequence from that of the rat erythrocyte enzyme. Sepiapterin reductase activity has not been found in crude extract of fat body from the silkworm mutant lemon. Although the antibody recognized only 29-kDa protein in the crude extract of silkworm fat body from normal strain after SDS-treatment, the antibody recognized only an approximately 80-kDa protein in the crude extract of the lemon mutant after SDS-treatment.

Alcohol Oxidoreductases↗
PubMed1977

A rapid screening method for blood glucose estimation.

A rapid method for estimating glucose concentrations in serum and in fluoride-, iodoacetate-, heparin-, and EDTA-treated plasma and whole blood is described. The procedure requires about three minutes to perform and utilize a tungstic acid precipitant solution and urine glucose dipsticks. Test results correlate with those of a reference quantitative glucose method at levels from 25 to 500 mg/dl (1.38 to 27.5 mmol/l). Hemolysis, lipemia and bilirubin levels as high as 20 mg/dl (342 mmol/l) do not interfere with the procedure. The simplicity and adaptability of the method make it useful in emergency situations.

Bilirubin↗
PubMed1977

Comparison of several methods for semiquantitative determination of urinary protein.

Two types of urine protein dipsticks and the sulfosalicylic acid method were compared for their accuracy and specificity, with use of urine samples supplemented with various proteins. Dipsticks yield accurate results when the protein under consideration is restricted to albumin; the sulfosalicylic acid method accurately determines many kinds of proteins in addition to albumin. Detergents affect each of the methods, but changes in salt concentration only affect results by dipstick procedures. Dipsticks, which are based on the protein-error principle for indicators, are subject to some of the conditions that apply to the bromcresol green method for serum albumin determination.

Albuminuria↗