PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 900068

A rapid screening method for blood glucose estimation.

W L Gyure
View original source ↗Export metadata ↓

Abstract

A rapid method for estimating glucose concentrations in serum and in fluoride-, iodoacetate-, heparin-, and EDTA-treated plasma and whole blood is described. The procedure requires about three minutes to perform and utilize a tungstic acid precipitant solution and urine glucose dipsticks. Test results correlate with those of a reference quantitative glucose method at levels from 25 to 500 mg/dl (1.38 to 27.5 mmol/l). Hemolysis, lipemia and bilirubin levels as high as 20 mg/dl (342 mmol/l) do not interfere with the procedure. The simplicity and adaptability of the method make it useful in emergency situations.

Explore related subjects

BilirubinBlood GlucoseChemical PrecipitationHumansLipidsMethodsComparative StudyJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

W L Gyure. 1977. A rapid screening method for blood glucose estimation.. https://doi.org/10.1093/ajcp%2F68.3.336

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1992

Conformation inversion of bilirubin formed by reduction of the biliverdin-human serum albumin complex: evidence from circular dichroism.

As shown by circular dichroism spectroscopy, biliverdin preferentially adopts an M-helicity conformation on human serum albumin in aqueous buffer, pH 7.5, whereas biliverdin exhibits only a weak preference for the P-helicity conformation on bovine serum albumin at the same pH. Upon rapid reduction of the complexes with sodium borohydride, P-helicity bilirubin-IX alpha is obtained on the human albumin complex, and M-helicity bilirubin-IX alpha is obtained on the bovine serum albumin complex. Thus, biliverdin in effect undergoes an inversion of chirality upon reduction. Since the reduction did not afford a rubin with the same helicity as that of the verdin, the observations point to a hitherto undetected conformational mobility of albumin-bound bilirubin.

Bilirubin↗
PubMed1992

Reduced albumin binding of MADDS--a measure of bilirubin binding--during pregnancy and delivery.

It was the purpose of the present study to investigate whether the decrease in the ability of plasma albumin to bind bilirubin, noted in pregnancy and during delivery, takes place in one step, during pregnancy alone, or in two steps, during both pregnancy and delivery. Furthermore, it was investigated whether a possible decrease during labor was related to the method of delivery itself. The material comprised (a) 17 pregnant women who delivered vaginally; (b) 25 women who were delivered by Cesarean Section; and (c) a group of 25 non-pregnant women. The reserve albumin concentration for binding of MADDS (a measure of the binding of unconjugated bilirubin), the total albumin concentration and the ratio between them were constant in the 6-week period up to birth and were significantly lower than the corresponding values in the non-pregnant group. During labor a significant decrease in reserve albumin and ratio of reserve albumin to total albumin was observed, while no change in the total albumin concentration was noted. No significant difference in reserve albumin concentration, total albumin concentration and ratio between them was found when the group of mothers who delivered vaginally was compared to the Cesarean Section group. It is concluded that the decrease in the ability of plasma albumin to bind unconjugated bilirubin takes place stepwise. The first step is a significant reduction during pregnancy followed by a further decrease during labor. The method of delivery is of no significance.

Bilirubin↗
PubMed1992

[Acute liver failure: current hepatological-surgical therapy strategies].

Patients with acute liver failure, characterized by deep jaundice, hepatic encephalopathy within eight weeks from the start of symptoms, are subjected to intensive care treatment. New drugs (Interferons, Prostaglandins) have been applied to these patients, with variable results. Mortality of fulminant hepatic failure is high in patients who don't respond to intensive therapy. These patients are characterized by progressive hepatic encephalopathy, decrease of clotting factors, especially of factor 5 and constantly increased serum bilirubin. Results of a multivariate analysis disclosed a poor prognosis in the individual patient more then 40 years old or under 11 years, with a prothrombin time below 10% of control, serum bilirubin over 300 mmol/l and with a late onset of hepatic encephalopathy after long standing jaundice.

Bilirubin↗