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Biomedical subjects

W Müller

Publications and source records attributed to W Müller.

At least 613 records · Page 34Linked to original sources
PubMed1987

[Reactive arthritis in enteral infections].

Certain gastrointestinal tract infections can trigger reactive arthritis. The best known triggering agents are yersinia, salmonella, shigella, campylobacter jejuni, and possibly clostridium difficile. The clinical findings show a subacute onset of mono- or oligoarthritis, less often of asymmetric polyarthritis with predominance of the lower limbs. The clinical picture is somewhat modified by the genetic constitution (HLA type) of the patient. HLA-B27-positive patients appear to have more severe symptoms, extraarticular features and incomplete or complete Reiter's syndrome than HLA-B27-negative patients. Diagnosis of postenteritic reactive arthritis is based on bacteriological stool analysis and/or examination of serumantibodies to the corresponding agents. Reactive arthritis is usually self-limiting and therefore treatment of the disease has to be mainly symptomatic. Only more severe chronic or destroying arthritis is thought to be treated locally (i.e. by synoviorthesis) or by immunoregulatory drugs.

Arthritis, Infectious↗
PubMed1987

[Basic research in ultrasound surgery. VII. Comparative animal experiments after intestinal anastomosis by suturing, ligament gluing with Fimomed and ultrasound sealing with ligament Fimomed].

Animal experimental anastomoses in the small intestine of Mini-Lewe-pigs showed that even sufficient anastomoses free of stenosis can be obtained by using the sticking respectively the ultrasound sealing method. The foreign-body reaction in the surroundings of the suture was approximately equally strong as on the gluten. All animals did not show any specialty in the postoperative course. The use of butylcyanidacrylic is possible in the human intestine as also shown in the literature. The use is indicated if advantages are to be expected in face of a suture anastomosis in clinical sight.

Anastomosis, Surgical↗
PubMed1987

[Examination of the injured knee joint].

Examination of the knee joint and the assessment of capsular ligament lesions require profound anatomical and biomechanical knowledge. Particular importance attaches to detailed information about the mechanism of the accident and to stability testing; these allow the establishment of diagnosis in the majority of cases. The diagnosis should take account of the anatomical structures damaged, thus defining both the location and the functional significance of the lesion, which are also important for therapeutic strategy. Arthroscopy makes it possible to solve almost all diagnostic problems and also to apply adequate therapeutic measures. This has necessarily had a considerable influence on strategies for the management of patients with knee injuries. Therapeutic considerations should include the critical evaluation of indications for surgery.

Arthroscopy↗
PubMed1987

[Ultrasonic sealing of the lung parenchyma after atypical resection].

Surfaces of lung parenchyma after resection or lesion can be sealed reliably by means of monomeric tissue adhesive with immediate polymerization by ultrasound sealing. No bronchopleural fistula was observed during the animal experimental testing. Necrotic areas of the lung parenchyma are avoidable extensively. This technique is simple. It offers reliability for patient and operator in emergency situations.

Animals↗
PubMed1987

[ARDS in Mycoplasma pneumonia].

In this paper we describe a case of bilateral broncho-pneumonia due to invasion by mycoplasma pneumoniae, leading to ARDS. A high serum level of cold agglutinins, a mild haemolysis and a rash resembling that of an erythema multiforme were noted. The initial treatment was non-specific. After pathogen identification tetracycline therapy was initiated. Bacterial superinfections were treated with appropriate antibiotic schemas. The observed biphasic clinical course could be explained as an immunological phenomenon.

Adult↗
PubMed1987

DNA pattern in choroid plexus papillomas.

Report on Feulgen-cytophotometric DNA studies of 8 choroid plexus papillomas. 7 tumours reveal distinct diploid stemlines in their karyogram. One tumour with oncocytic transformation exhibits a stemline in the triploid stage. These findings reflect the DNA pattern of a benign euploid neoplasm of the there investigated tumours.

Adolescent↗
PubMed1987

A boy with recurrent infections, impaired PMN-chemotaxis, increased IgE concentrations and cranial synostosis--a variant of the hyper-IgE syndrome?

A patient with coarse facies, craniosynostosis, recurrent staphylococcal infections with pneumatocele formation is described. Laboratory features included moderately elevated serum IgE, cutaneous anergy, decreased numbers of T-suppressor cells and variable inhibition of neutrophil chemotaxis. The combination of clinical findings suggests the diagnosis hyper-IgE syndrome, though the total IgE serum concentration (800 U/ml) and the level of IgE-specific antibodies to staphylococci (9.4%, normal less than 5%) were only slightly elevated.

Chemotaxis, Leukocyte↗
PubMed1986

[Rheumatoid factors of immunoglobulin classes IgA, IgG and IgM: methods of determination and clinical value].

Routine laboratory tests (Rose-Waaler test, latex fixation test) for the detection of rheumatoid factor (RF) measure IgM RF by its ability to agglutinate sheep red blood cells or latex particles coated with IgG. Recently, solid-phase RIA and ELISA have been developed to detect RF of all major immunoglobulin classes in a sensitive and reproducible manner. In the literature the pathogenetic, diagnostic and prognostic significance of IgA, IgG and IgM RF is discussed. Using a micro-ELISA with human IgG-Fc-fragments as antigens, we have detected IgA RF and/or IgG RF not only in sera from seropositive rheumatoid arthritis (RA) (n = 48), but also in 13 of 22 sera from seronegative RA, which was statistically significant compared with a group of 50 normal controls. There was a good correlation between IgM RF in ELISA and Rose-Waaler titres for agglutinating RF (r = 0.70). No RF were found in patients with psoriatic arthritis (n = 16).

Adult↗
PubMed1986

Slow cholinergic excitation of guinea pig hippocampal neurons is mediated by two muscarinic receptor subtypes.

Stimulation of cholinergic fibers or bath application of carbachol (0.1-10 microM) induced a slow excitability increase in CA3 neurons and dentate granule cells of hippocampal slices. This effect which was antagonized by atropine (1 microM) was mediated by two receptor subtypes: a pirenzepine (10 microM)-insensitive receptor, 'M2', and a pirenzepine (1 microM)-sensitive receptor, 'M1'. The M2-receptor activation led to a blockade of slow afterhyperpolarizations following trains of action potentials and to the occurrence of threshold-activated plateau-depolarizations associated with a conductance increase. The M1-receptor mediated a membrane depolarization sometimes associated with a conductance decrease which reversed its polarity at membrane potentials negative to -80 mV. The 'slow excitatory postsynaptic potential' which results from activation of cholinergic fibers is thus caused by the activation of two receptor subtypes.

Animals↗
PubMed1986

Lymphokines regulate immunoglobulin isotype expression in an antigen-specific immune response.

The control of immunoglobulin class switching appears to involve T cell-derived lymphokines. Such lymphokines have been shown to affect isotype expression in polyclonally activated B cells. We show in this paper that the same lymphokines similarly control isotype expression in an antigen-specific response acting in concert with a "T cell independent" antigen. In this situation, B cell growth factor II (BCGF II) enhances the production of antigen-specific IgM antibodies, whereas the production of antigen-specific IgG1 antibodies is only observed in the presence of B cell differentiation factor gamma (BCDF gamma). These results suggest that these lymphokines (and perhaps additional ones) are involved in the control of isotype expression in antigen-specific responses.

Animals↗
PubMed1986

[Significance of leukotrienes in chronic respiratory tract diseases in childhood].

The role of lipoxygenase products was studied in children suffering from chronic diseases of the lung. Leukotrienes C4, D4, E4 and B4 were measured by high performance liquid chromatography (HPLC) and a specific radioimmunoassay (RIA) for C4. Elevated levels (up to 40 ng/ml), especially for leukotriene E4, were found in plasma of asthmatic and bronchitic patients (leukotriene C4 concentrations varied between 0.05 and 40 ng/ml, mean 4.9 +/- 7.8 ng/ml). In healthy donors the concentrations were below the detection limits of HPLC, leukotriene C4 ranging between 5 +/- 4 ng/ml (RIA data). The conversion of leukotriene C4 to D4 and E4 was observed by incubating the samples with synthetic leukotriene C4. The half-life of leukotriene C4 in plasma varied greatly, ranging from less than 12 min to 72 min (mean 39 +/- 16 min). Bronchial lavages yielded leukotriene C4 concentrations of 0.2 to 7 ng. Leukotriene E4 was detected in 10 of 41 cases. Conversion of leukotriene C4 did not occur in 50% of all cases, but was regularly observed in putrid lavages. These data suggest that leukotrienes play an important role in allergic and infectious lung diseases.

Adolescent↗
PubMed1986

Fractionation of DNA restriction fragments with ion-exchangers for high-performance liquid chromatography.

The fractionation abilities of several ion-exchangers of the high-performance liquid chromatography type for two sets of DNA restriction fragments, ranging from 7 base pairs (bp) to about 650 bp and differing in their mean base composition, have been studied. The ion-exchangers tested comprise the RPC-5, the 5-PW DEAE and the Mono Q as polymer-based resins, and the Nucleogens 500 and 4000, both prepared from silica beads. The results indicate that all the ion-exchangers except the 5-PW DEAE perfectly separate fragment sizes up to about 90 bp, the 5-PW DEAE separating to 45 bp only. Above 200 bp only the Mono Q resin works in a satisfactory way provided that about 100 micrograms DNA mixture, containing less than 25 fragments within the given size range, is loaded per milliliter of packed resin. Appreciable base-pair specificities were detected for most of the resins which cause substantial retardations of the d(A + T)-rich fragments with respect to the eluting salt concentration. If the latter dominate in the DNA sample, acceptable results were only obtained with the Mono Q resin when the column was operated at elevated temperature.

Base Composition↗