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Biomedical subjects

W McLean

Publications and source records attributed to W McLean.

27 records · Page 2Linked to original sources
PubMed1985

DNA-mediated transfer of human melanoma cell surface glycoprotein gp130: identification of transfectants by erythrocyte rosetting.

DNA sequences encoding a human melanoma membrane-bound sialoglycoprotein of 130,000 molecular weight (gp130) were introduced into a clonal derivative of mouse B-16 melanoma cells with the selectable neomycin resistance gene (aminoglycoside phosphotransferase). Mouse transfectants were identified by a rapid and precise screening method with mouse monoclonal antibodies and erythrocyte rosetting. The frequency of gp130 transfectants was approximately 1 in 2,000 to 5,000 colonies with neo+ cells. Analysis of secondary mouse transfectants has revealed that the transfected gp130 has a molecular weight, isoelectric point, intracellular processing, peptide map, and spatial orientation of surface-exposed epitopes indistinguishable from those seen with gp130 from human melanoma cells. In contrast to primary transfectants, secondary transfectants expressing gp130 lack demonstrable human repetitive sequences.

Animals↗
PubMed1983

Primary amebic meningoencephalitis diagnosed in the emergency department.

Reported is a case of primary amebic meningoencephalitis diagnosed in the emergency department. The patient, a previously healthy teenager, developed Naegleria meningoencephalitis after swimming in a freshwater public pool. The Naegleria caused acute fulminating infection culminating in the death of the patient 36 hours after admission. Results of a spinal tap, together with the history of swimming in warm fresh water, led to the emergency department diagnosis.

Adolescent↗
PubMed1980

Necrotizing vasculitis in gynecological surgery.

Extensive clinical investigation of asymptomatic vasculitis found in routine histopathological studies following gynecological surgery may often be unnecessary. In 11 patients necrotizing angiitis was an incidental finding in surgically removed uterine and uterine adnexal tissues. Following irradiation for urinary bladder carcinoma, one patient died during radical surgery: vasculitis was found in surgically resected pelvic organs, colon and kidneys. In other patients, vasculitis may have been attributable to drugs, e.g. penicillin, or to local arthus-like reaction following cone biopsy of cervix. Available from 6 of these cases, follow-up data presented no evidence of progressive systemic vascular disease, supporting the view that incidentally encountered necrotizing angiitis of uterus and uterine adnexa, like that of the appendix, may be innocuous.

Adult↗
PubMed1980

Taurine transport in epilepsy.

Previous studies of plasma taurine concentrations in epileptics have yielded equivocal results. We measured plasma and urinary taurine in 41 epileptic and 68 control subjects and found plasma concentrations among epileptics to be comparable in general to those of controls, but that two or three classes of plasma taurine concentrations, possibly genetically regulated, occur in both epileptic and control subjects. Our previous studies and data from the present study on taurine excretion revealed three excretion classes under genetic control. The principal finding is that epileptics include disproportionate numbers of low excretors (high reabsorbers), who are presumptive homozygotes for the allele effecting higher reabsorption. If confirmed, these findings suggest that the transport of taurine, rather than absolute taurine concentration, may explain the efficacy of taurine administration in some epileptics but not in others. The locus involved may be one component in the polygenic diathesis to the idiopathic epilepsies.

Child↗
PubMed1979

An epilepsy workshop for professionals.

This experimental study evaluated the impact of an epilepsy workshop format designed for community professionals who may be involved in the treatment plan and follow-up of patients. The goals of the workshop were to increase the professional's knowledge and comfort in the presence of epilepsy and to effect better delivery of services to patients. To achieve this, a didactic approach to learning was modified to include patient contact and participant involvement in the learning process. A pre- and posttest experimental design was utilized. Those who attended the workshop improved significantly more than the two control groups in their knowledge of and comfort with epilepsy. Following the workshop, there was a marked increase in the referral of school children for possible neurological problems by teachers who had attended. There was a subsequent improvement in the delivery of health care services to such children in this county.

Adolescent↗
PubMed1976

Secretion and role of androgen-binding proteins in the testis and epididymis.

The Sertoli cell appears to be a target cell for both FSH and androgen. FSH stimulates the Sertoli cell to produce an androgen-binding protein (ABP) which may serve to increase the accumulation of androgen in the seminiferous tubular epithelium and make it available for binding by intracellular androgen receptors. This might be one way by which FSH enhances the action of androgen on spermatogenesis. Androgen acts on the Sertoli cell to increase its response to FSH resulting in increased production of ABP and of other substances which exert trophic effects on spermatogenesis.

Androgens↗
PubMed1975

New systems for a two-speed electroencephalograph with an auditory device for diminishing the length of seizures.

The author describes a system for testing patients with petit mal seizures. The system has the following advantages: 1) EEG paper speed is automatically increased from 2.5 mm/s to 25 mm/s when brainwave activity increases, with the increased speed maintainable for up to 4.0 sec after the last seizure burst. Control studies with constant speed and two-speed systems operating simultaneously demonstrate that no significant distortion occurs at the moment of change in speed. The convenience of handling a greatly reduced volume of paper is obvious. 2) An auditory stimulus, either a pure tone burst of 1000 HZ or loud music, is also triggered by an increase in amplitude of brainwave activity. Testing of 15 patients shows a significant decrease (p less than 0.01) in duration of seizure activity when the stimulus is activated.

Acoustic Stimulation↗
PubMedDate not supplied

One hospital's success with medication budget control.

In the era of major restraint of health care spending in Ontario, the Ottawa General Hospital, a 530-bed adult tertiary care facility, initiated a series of programs aimed at reducing their drug costs by $500,000 over 18 months; the goal was achieved in 12 months. This success is attributed to the collaboration of key medical staff, the pharmacy department and senior administration. Overall, quality of care was unaffected and was perhaps improved.

Budgets↗