Biomedical subjects

W Scheuchenzuber

Publications and source records attributed to W Scheuchenzuber.

4 recordsLinked to original sources

PubMed2006

Technique for rapid in vitro single-cell elastography.

Statistically meaningful assays require scanning a large number of cells in a short time. Here we present a technique for rapid in vitro single-cell elastography. The technique is based on atomic force acoustic microscopy but (1) requires only a few minutes of scan time, (2) can be used on live cells briefly removed from most of the nutrient fluid, (3) does negligible harm or damage to the cell, (4) provides semiquantitative information on the distribution of modulus across the cell and (5) yields data with 1- to 10-nm resolution. We describe the new technique in detail, apply it to baby hamster kidney cells, verify the results and calibrate the images with atomic force microscope force-distance measurements. The technique enables rapid assessment of physical/biochemical signals on the cell modulus and contributes to current understanding of cell mechanics.

Animals↗

PubMed2000

Production and evaluation criteria of specific monoclonal antibodies to the hemagglutinin of the H7N2 subtype of avian influenza virus.

To enhance the rapidity in diagnosing the spread of avian influenza virus (AIV) in chicken layer flocks, studies were initiated to develop more sensitive and specific immunological and molecular methods for the detection of AIV. In this study, the purification of the hemagglutinin protein (H) from field isolates of H7N2, the production of monoclonal antibodies (MAbs), and their evaluation as diagnostic reagents are reported. Hybridomas were generated by fusion of SP2/0-Ag14 myelomas and spleen cells from immunized mice. Hybridomas secreting antibodies specific for the H protein were assayed by an ELISA and cloned using limiting dilution. The MAbs produced were characterized by hemagglutination inhibition (HI), immunohistochemistry (IHC), indirect fluorescent antibody assay (IFA), Western blots, and IFA flow cytometry using various AIV subtypes (i.e., H4N2, H5N3, H7N2). Of the various MAbs assayed, 6 had consistent and reproducible results in each of the assays used. The results obtained in this investigation enhanced the usage of the MAbs to viral H protein in the surveillance of AIV in chickens.

Animals↗

PubMed1987

Porcine effector mechanisms: antibody-dependent cell-mediated cytotoxicity of pseudorabies-infected target cells.

Antibody-dependent cell-mediated cytotoxicity (ADCC) against pseudorabies virus (PRV)-infected cells was determined in neonatal porcine pulmonary washout cells and in partially purified blood-derived leukocytes. The ADCC assays were performed at approximately 1, 2, 3 and 11 days after birth. It was found that pulmonary-derived cells had a low level of cytolytic activity against the PRV-infected target cells at about 1 day of age. This activity increased sharply during the next 2 days of age, but declined somewhat by 11 days of age. The increase in ADCC activity paralleled closely the increase in the relative and absolute numbers of macrophages in the alveolar washout fluids. ADCC activity was high at all times in blood-derived neutrophils, and present in blood-derived lymphocytes, but at a lower level of activity.

Animals↗

PubMed1985

Antioxidant effects on cell-mediated immunity.

Experiments were performed to determine the effects of dietary selenium and/or vitamin E deficiency on cell-mediated cytotoxicity in the mouse. Natural killer cell-mediated cytotoxicity (NKCC) was depressed after 8 wk on diets deficient in selenium and/or vitamin E. In contrast, antibody-dependent cell-mediated cytotoxicity (ADCC) was not affected by 8 wk of dietary deficiency of selenium and/or vitamin E. T-lymphocyte-mediated cytotoxicity (TCMC) was found to be depressed by combined selenium-vitamin E deficiency after 7 weeks on diets.

Animals↗