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Włodzimierz Doroszkiewicz

Publications and source records attributed to Włodzimierz Doroszkiewicz.

9 recordsLinked to original sources
PubMed2006

A comparison of the in vitro antimicrobial activity of liposomes containing meropenem and gentamicin.

The antimicrobial activity of eight cationic, two neutral and three anionic liposome compositions containing meropenem and gentamicin was tested in vitro in broth and serum medium. The cationic formulations showed better antibacterial efficacy against both Gram-positive and Gram-negative bacteria than the anionic and neutral ones, regardless of the encapsulated drug. The most effective formulations were the cationic PC/DOPE/DOTAP 3:4:3 and PC/Chol/DOTAP 3:4:3, as the MICs with meropenem were 2 to 4 times lower than those of the free drug.

Anti-Bacterial Agents↗
PubMed2005

Potential possibilities of using phage typing in elimination of multidrug resistant staphylococci.

Coagulase-negative staphylococci (CoNS) have become the most often isolated bacteria from blood culture, spinal fluid and respiratory tracts of neonates. These nosocomial strains are often resistant to oxacillin and other antibiotics (macrolides, aminoglycosides and fluorochinolones). 50 multidrug resistant CoNS strains isolated from bloodstream neonatal infections were tested for sensitivity to 23 lytic staphylococcus bacteriophages. No lytic patterns for 19 of the phages were observed. Phages P4, A3R and 676/Z were active against 46%, 54% and 56% of the strains, respectively. In general, 60% of CoNS isolates were susceptible to one or more of the staphylococcus bacteriophages.

Anti-Bacterial Agents↗
PubMed2005

Differentiated pattern of protein composition of crystalline inclusions of newly isolated Bacillus thuringiensis strains from Silesia in Poland.

Protein profiles of crystal delta-endotoxins were determined in twenty nine Bacillus thuringiensis strains-soil and phylloplane isolates--from Poland. Electrophoretic analysis revealed quantatively and qualitatively different patterns of delta-endotoxin crystal preparations of these B. thuringiensis strains. The crystalline parasporal inclusions of B. thuringiensis isolates were composed of two, three, four or five proteins. Molecular weights of these polypeptides varied from 23.4 kDa to 142 kDa. There is lack of correlation between serovars of B. thuringiensis strains, the morphology of crystals and the number and size of proteins in parasporal inclusions.

Bacillus thuringiensis↗
PubMed2005

The susceptibility of anaerobic bacteria isolated from periodontal diseases to photodynamic inactivation with Fotolon (chlorin e6).

Photodynamic inactivation (PDI) may be a very promising alternative method for the antimicrobial treatment of periodontitis. Several studies have demonstrated the sensitivity of subgingival flora to PDI using toluidine blue, methylene blue, and chlorin e6 derivatives. In the present study we report the activity of the Fotolon sensitizer, composed of chlorin e6 and polyvinylpyrrolidone (PVP), against anaerobic bacteria isolated from periodontal diseases. Over 99.9% reduction in colony forming units in 20 Gram-positive and 30 Gram-negative clinical anaerobic strains was obtained.

Bacteria, Anaerobic↗
PubMed2004

[Bactericidal activity of normal human serum to gram-negative rods with sialic acid containing lipopolysaccharide].

The complement plays the most important role in eliminating bacterial invasion of the host, by facilitating phagocytosis of potential pathogens and by participating in the direct essential role in protecting gram-negative bacteria against bactericidal activity of serum. Sialic acids which are important constitutes of animal tissue glycoconjugates are also present in antigens of some bacterial strains. The susceptibility of gram-negative strains with sialic acid--containing lipopolysaccharides to bactericidal action sera was examined.

Blood Bactericidal Activity↗
PubMed2003

[Evaluation of sensitivity to antibiotics of microorganisms isolated from children with urinary tract infections].

The incidence of etiological factors of urinary tract infections in children and in vitro susceptibility of these strains to antibiotics were evaluated. 1082 strains isolated from 905 samples of urine of children hospitalized in the period of 01.01-31.12.2001 were tested. Among the isolated microorganisms, the most common group was the Enterobacteriaceae family (57.4%) and also very often Gram-positive cocci were isolated (34.2%). The most frequent causative agents of UTI (Escherichia coli and Enterococcus faecalis) were generally sensitive to all used antibiotics, but E. coli rods were resistant to ampicillin. In addition, 23.4% of E. coli were the ESBL-producing strains.

Anti-Bacterial Agents↗
PubMed2003

The factor C3 conversion in human complement by smooth Shigella flexneri lipopolysaccharides.

Shigella flexneri rods play an important role in human intestinal infections. In the presented studies we have shown that O-acetyl and glucose residues, substituted in main GalNAc-Rha chains of lipopolysaccharide (LPS) are important for the bactericidal effect of human serum. By dot-blot, immunoblotting and ELISA with immobilized LPS we have shown correlation of C3 fragments deposition and serum resistance. LPSs isolated from a serum-sensitive strain deposited more C3 fragments than LPSs from serum-resistant Shigella flexneri strains.

Blood Bactericidal Activity↗
PubMed2002

[The sensitivity of the uropathogenic Escherichia coli strains to antibiotics, bacteriophages and bactericidal serum activity].

Escherichia coli strains isolated from children with urinary tract infections (UTI) were investigated for their sensitivity to antimicrobial drugs, bacteriophages and bactericidal activity of human serum. It has been proved, that the resistance to bactericidal effect of serum is not the dominant feature of uropathogenic Escherichia coli strains. Significant percentage of the strains appeared to be sensitive to the most popular drugs ordered during UTI treatment in children. No simple relationship between sensitivity of the strains to the drug and to the human serum has been found. Three of 44 bacteriophages specific to Escherichia coli have shown the lytic effect towards 50-60% strains under investigations.

Anti-Infective Agents, Urinary↗