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PubMed · 12916727

The factor C3 conversion in human complement by smooth Shigella flexneri lipopolysaccharides.

Rafał Fudała·Włodzimierz Doroszkiewicz·Joanna Niedbach·Andrzej Gamian·Andrej Weintraub·Wiesław Kaca
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Abstract

Shigella flexneri rods play an important role in human intestinal infections. In the presented studies we have shown that O-acetyl and glucose residues, substituted in main GalNAc-Rha chains of lipopolysaccharide (LPS) are important for the bactericidal effect of human serum. By dot-blot, immunoblotting and ELISA with immobilized LPS we have shown correlation of C3 fragments deposition and serum resistance. LPSs isolated from a serum-sensitive strain deposited more C3 fragments than LPSs from serum-resistant Shigella flexneri strains.

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Blood Bactericidal ActivityCarbohydrate SequenceComplement ActivationComplement C3Complement Hemolytic Activity AssayDysentery, BacillaryEnzyme-Linked Immunosorbent AssayHumansImmunoblottingLipopolysaccharidesMolecular Sequence DataO AntigensShigella flexneriJournal ArticleResearch Support, Non-U.S. Gov't

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Rafał Fudała, Włodzimierz Doroszkiewicz, Joanna Niedbach, Andrzej Gamian, Andrej Weintraub, Wiesław Kaca. 2003. The factor C3 conversion in human complement by smooth Shigella flexneri lipopolysaccharides.. https://pubmed.ncbi.nlm.nih.gov/12916727/

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