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Biomedical subjects

X Fu

Publications and source records attributed to X Fu.

At least 217 records · Page 12Linked to original sources
PubMed1992

Multiple organ injuries after abdominal high energy wounding in animals and the protective effect of antioxidants.

Multiple organ injuries caused by high energy abdominal wounds were studied in 8 pigs and 24 dogs, and at the same time the protective effect of antioxidants in 14 dogs with multiple organ injuries was also studied. The experimental results showed that: 1) more than two organs (six organs at most) were wounded in each of the animals studied; 2) the injuries were characterized by hemorrhaging, tissue rupture and hematoma, and the main pathologic changes were local edema and necrosis; 3) the marked increase of lipid peroxide (LPO) levels in the vital organs indicated that multiple organ injuries could also involve the molecular level; 4) the injuries were due to the direct effect of pressure waves and ischemic reperfusion and not to shock or infection; and 5) antioxidants (vitamin E and Salvia miltiorrhiza Bge.) exhibited significant protective effects against multiple organ injuries through a free radical mechanism.

Abdominal Injuries↗
PubMed1992

[Interphase cytogenetic studies of human X chromosome].

The chromosome in situ hybridization with human X chromosome alpha satellite DNA probe (pBamX7) on human lymphocyte metaphases and interphase nuclei was performed for interphase cytogenetic studies. The individuals with numerical or structural abnormalities of X chromosome were studied. The results showed that the probe hybridized specifically to the centromeric region (p11----q11) of X chromosome. The number of silver grain clusters in interphase nuclei was correlated with that of X chromosome. Most of the clusters located near the nuclear membrane where inactive X chromatins (Barr-bodies) were usually found. The method of ascertaining the number of X chromosomes by in situ hybridization was much more reliable than that by counting the number of Barr-bodies. The modified R-banding technique was introduced and the significance of this work was also discussed.

Chromosome Aberrations↗
PubMed1991

[Relation between lipid level and gallstone].

Serum total cholesterol (TC), triglyceride (TG), beta-lipoprotein (beta-L), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), ratio of HDL-C to TC and LDL-C to HDL-C, age and sex were compared among 252 patients with gallstones (gallstone group, 76 males, 176 females) in gallbladder and 399 controls (256 males, 134 females, control group), who were identified by ultrasonography in a general check-up during the same period. The high risk factors of gallstone were analyzed by stepwise logistic regression. The increase of beta-L concentration (P less than 0.001), female (P less than 0.001), and high ratio of LDL-C to HDL-C (P = 0.0087) were suspected as the high risk factors of gallstone formation in gallbladder.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

Phosphorylation of avian retrovirus matrix protein by Ca2+/phospholipid-dependent protein kinase.

The matrix protein from avian myeloblastosis virus and the Rous sarcoma virus, Prague C strain, is a phosphoprotein. A comparison of the amino acid sequences shows these phosphoproteins are very similar. The sites of phosphorylation of the matrix protein purified from virions are identified as serine residues 68 and 106. Treatment with purified rabbit skeletal-muscle protein phosphatase 1 or 2A, selectively releases phosphate from serine 68, while alkali treatment releases phosphate from both sites. When analyzed as a substrate for six different protein kinases, only the Ca2+/phospholipid-dependent protein kinase modifies the matrix protein. The serine residues phosphorylated in vivo are identical to those phosphorylated in vitro by this protein kinase. The role of these phosphorylation events in viral production is discussed.

Amino Acid Sequence↗
PubMed1989

Ten families of ankylosing spondylitis.

Twenty-two patients with ankylosing spondylitis (AS) from 10 families were studied with special attention to their clinical findings, HLA-B27 examinations and family histories. Results showed that HLA-B27 was positive in 19 and negative in 3. The hereditary relationship between ankylosing spondylitis and HLA-B27 in Chinese was similar to that in westerners. We consider that subjective symptoms and clinical findings are essential to early diagnosis of AS, but HLA-B27 examination and family history are supportive.

Adolescent↗
PubMed1985

Site-specific phosphorylation of avian retrovirus nucleocapsid protein pp12 regulates binding to viral RNA. Evidence for different protein conformations.

Phosphorylation of serine 40 of the major nucleocapsid protein of avian retroviruses, pp12, regulates binding to viral RNA (Leis, J., Johnson, S., Collins, L. S., and Traugh, J. A. (1984) J. Biol. Chem. 259, 7726-7732). The phosphorylation state of the protein can be altered in vitro, resulting in the interconversion of the protein between a state of high affinity for single-stranded RNA and low affinity for single- or double-stranded RNA. The reversible phosphorylation of serine 40 is accompanied by a change in the conformation of the protein as demonstrated by quenching of intrinsic tryptophan fluorescence and chemical modification studies. Quenching of fluorescence of the sole tryptophan residue, Trp 80, by poly(U), KI, and CsCl indicates that the microenvironment of this residue is more positive in pp12 than in p12. Chemical modification studies indicate that the 3 lysine residues at positions 36, 37, and 39 of pp12 react with 2,4,6-trinitrobenzenesulfonic acid, while only 1 of these residues reacts in p12. The addition of single-stranded, but not double-stranded RNA, to pp12 protects 2 of the 3 lysine residues from chemical modification, suggesting that the two protected lysyl groups are required for binding to single-stranded viral RNA. In contrast to the phosphorylation of serine 40, phosphorylation of serine 43, catalyzed by protease-activated kinase II in vitro, does not induce changes in the protein conformation nor does it alter the RNA binding properties of the protein.

Amino Acid Sequence↗
PubMedDate not supplied

Acidic fibroblast growth factor reduces renal morphologic and functional indicators of injury caused by ischemia and reperfusion.

Therapeutic effects of acidic fibroblast growth factor on postischemic renal injury were evaluated in a rat model of bilateral renal ischemia (60 minutes) and reperfusion (7 days). Twenty-four rats were randomly divided into two groups (12 rats each). After 60 minutes of ischemia and at the onset of reperfusion, rats in the acidic fibroblast growth factor-treated group received 2.6 microg of acidic fibroblast growth factor/rat in 50 microl of phosphate-buffered saline solution containing 0.1% heparin (w/v) through the jugular vein, whereas the rats in the phosphate-buffered saline solution-treated group received the same vehicle without acidic fibroblast growth factor. Compared with the phosphate-buffered saline solution-treated group, rats in the acidic fibroblast growth factor-treated group had significantly lower blood urea nitrogen (83.13 +/- 26.07 versus 176.36 +/- 62.36, p < 0.05) and serum creatinine (0.73 +/- 0.14 versus 1.14 +/- 0.36, p < 0.05) levels 1 day after occlusion. Histopathologic scores showed much less renal damage on day 1 in the acidic fibroblast growth factor-treated rats compared with the phosphate-buffered saline solution controls. We conclude that intravenous administration of acidic fibroblast growth factor offers significant protection against postischemic renal injury and these protective effects may come from its nonmitogenic effects such as the regulation of vessel tone and calcium concentration in the body.

Journal Article↗
PubMedDate not supplied

Ischemia and reperfusion reduce the endogenous basic fibroblast growth factor in rat skeletal muscles: an immunohistochemical study.

Polyclonal antibodies directed against human recombinant basic fibroblast growth factor were used in immunohistochemical studies to localize this growth factor in normal and wounded rat skeletal muscles. According to the intensity of the stain, three main classes of fibers could be identified: the strongly, moderately, and weakly stained fibers. Basic fibroblast growth factor immunoreactivity was found mainly in the extracellular matrix, primarily in the endomysium, which includes the heparin-containing basal lamina, and also in the capillary basal membrane of both normal and wounded muscles; however, the signal intensity was much stronger in normal muscles. The distribution of basic fibroblast growth factor in wounded muscles became markedly heterogeneous and sparse. After 4 hours of ischemia, about 40% of skeletal muscle fibers lost their basic fibroblast growth factor immuno-reactivity. Muscles which underwent 4 hours of ischemia and 24 hours of reperfusion had only a diminished basic fibroblast growth factor immunoreactivity. The pathologic results supported the concept of destroyed cell connection and fiber necrosis in ischemic and reperfused muscles. Potential mechanisms involved in this reduced concentration of basic fibroblast growth factor in wounded muscles may include oxygen free radical activation, a generalized effect of the inflammatory response, and reduced secretion of endogenous basic fibroblast growth factor. These results are only partially compatible with the established mitogenic role of this growth factor and suggest that a reduction of endogenous fibroblast growth factor may partly contribute to a delay in wound healing.

Journal Article↗
PubMedDate not supplied

Epidemiological study of chronic dermal ulcers in China.

A total of 30,000 hospitalized surgical patients in 15 hospitals were screened for chronic ulcers. A total of 489 patients with chronic dermal ulcers were found with their major causes of ulceration including traumatic wounds, infections, diabetes mellitus, and venous diseases. Patients with chronic ulcers following trauma and infection comprised 67.48% of the total patient population. The incidence of diabetic ulcers and venous ulcers was 4.91% and 6.54% respectively. Sites of ulceration differed with different etiological factors. The percentage of chronic dermal ulcers in the lower extremities, upper extremities, thorax and abdomen, back, and head was 63.10%, 17.93%, 7.76%, 4.83% and 6.38% respectively. Of the 489 patients with chronic ulcers, 183 were farmers (37.42%), and 131 were workers (26.79%). Chronic dermal ulcers were more common in men than in women, but there was no significant difference in the sex-related prevalence. According to these data from different hospitals, the incidence of chronic ulcers in patients hospitalized for surgery was 1.5% to 3.0%. These data have primarily shown the prevalence and clinical characteristics of chronic ulcers in hospitalized patients in China. These data may not be consistent with reports from other countries. Significant differences in etiological factors of ulceration, professional distribution of patients with chronic dermal ulcers, and treatment methods were found when compared with reports of studies conducted in developed countries. Our results will benefit not only additional basic research, but these data will also be useful in preventing and managing chronic wounds in developing countries.

Adolescent↗
PubMedDate not supplied

Advances in wound healing research in China: from antiquity to the present.

China is the largest developing country in the world with wounds occuring everyday and everywhere, representing one of the main killers of people. Prevention and treatment of wounds are the main tasks for Chinese surgeons. Although wound care and management have a long history in China, wound healing research and management have made significant progress only in recent years. In this article we give a brief account of the history of wound care and management in China. In addition, we introduce the main research fields and achievements in tissue repair and regeneration during recent times in this country. We hope that these works will benefit our foreign colleagues to know our work and enhance the communication among us.

Animals↗
PubMedDate not supplied

Antiproliferative activity and toxicity of 2-methoxyestradiol in cervical cancer xenograft mice.

2-methoxyestradiol (2-ME) is considered to be an effective anticancer compound for many types of tumors. We have previously demonstrated that 2-ME inhibits the growth of human cervical cancer HeLaS3 cells in vitro. In this study, we investigated the antitumoral effects of 2-ME on human cervical carcinoma in severe combined immune deficient (SCID) mice. The potential side effects of 2-ME on the SCID mice were also investigated. SCID mice were injected with HeLaS3 cells (3 x 10(6) to 4 x 10(6)/mouse) and a 15-day administration of 2-ME followed after a 1-week cell implantation. Tumor weight, volume, body weight, and blood chemistry were determined. Tumor tissues were examined with an antibody against the proliferative cell nuclear antigen and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining. Liver, spleen, kidney, heart, and lung were screened by pathologic examinations. 2-ME (75 mg/kg p.o.) inhibited growth of human cervical carcinoma by 34% (P < 0.05) as compared with control. Necrosis was found in both 2-ME-treated and untreated tumor tissues, but the necrotic area was larger in 2-ME-treated mice. A low expression of proliferative cell nuclear antigen and an increased number of apoptotic cells were found in 2-ME-treated tumor sections as compared to those in controls. No significant difference was detected in blood chemistry. In addition, the liver showed hyperplastic Kupffer cells, hydropic swelling of hepatocytes, and liquefactive necrosis. The spleen showed an increased number of megakaryocytes and apoptotic cells after 2-ME treatment. Thus, 2-ME has an antitumor effect on human cervical carcinoma, and it is toxic to liver and spleen in this mouse model.

2-Methoxyestradiol↗
PubMedDate not supplied

A new patient-like metastatic model of human small-cell lung cancer constructed orthotopically with intact tissue via thoracotomy in nude mice.

A new nude-mouse metastasizing orthotopic transplant model of human small-cell carcinoma of the lung is described. Histologically-intact human small-cell lung tumors were transplanted to the left lung of nude mice via a thoracotomy procedure we have developed. The transplanted tumors grew extensively locally and metastasized to the opposite lung, lymph nodes and other clinically-relevant sites. The results described indicate the model developed could have clinical relevance and contrasts with models of small-cell carcinoma constructed with injections of cell suspensions which result in few or no metastases.

Animals↗
PubMedDate not supplied

Extensive liver metastasis from human colon cancer in nude and scid mice after orthotopic onplantation of histologically-intact human colon carcinoma tissue.

Clinically-relevant animal models of human cancer are greatly needed for the study of human cancer biology and the development of new cancer therapeutics and diagnostics. We report here that by orthotopically transplanting histologically-intact human colon cancer to the colon of the immunodeficient nude and scid mouse mutants that extensive local growth and liver metastases occur consistently even after extensive in vivo orthotopic passage. We demonstrate that the liver metastases arise by hematogenous spread. The models described in this report for human colon cancer should prove useful for individual cancer patients as well as for basic and applied studies to develop improved treatment.

Animals↗
PubMedDate not supplied

A patient-like metastasizing model of human lung adenocarcinoma constructed via thoracotomy in nude mice.

A new nude-mouse metastasizing orthotopic transplant model of human adenocarcinoma of the lung is described. Histologically-intact human A549 adenocarcinoma lung tumors were transplanted to the left lung of nude mice via a thoracotomy procedure we have developed. The transplanted tumors grew extensively locally and metastasized to the opposite lung, lymph nodes and other clinically-relevant sites. The results described indicate the model developed could have clinical relevance and contrast with models of the A549 lung adenocarcinoma constructed orthotopically with injections of cell suspensions which result in low metastatic potential.

Adenocarcinoma↗