Biomedical subjects

Xiao-Yan Zhou

Publications and source records attributed to Xiao-Yan Zhou.

5 recordsLinked to original sources

PubMed2006

[Evaluating training programs on occupational health and safety: questionnaire development].

OBJECTIVE: To develop a questionnaire to evaluate the quality of training programs on occupational health and safety. METHODS: A questionnaire comprising five subscales and 21 items was developed. The reliability and validity of the questionnaire was tested. Final validation of the questionnaire was undertaken in 700 workers in an oil refining company. RESULTS: The Cronbach's alpha coefficients of the five subscales ranged from 0.6194 to 0.6611. The subscale-scale Pearson correlation coefficients ranged from 0.568 to 0.834 . The theta coefficients of the five subscales were greater than 0.7. The factor loadings of the five subscales in the principal component analysis ranged from 0.731 to 0.855. Use of the questionnaire in the 700 workers produced a good discriminability, with excellent, good, fair and poor comprising 22.2%, 31.2%, 32.4% and 14.1 respectively. Given the fact that 18.7% of workers had never been trained and 29.7% of workers got one-off training only, the training program scored an average of 57.2. CONCLUSION: The questionnaire is suitable to be used in evaluating the quality of training programs on occupational health and safety. The oil refining company needs to improve training for their workers on occupational health and safety.

Education↗

PubMed2006

[Effect of trace element on the growth of white rot fungus and suppressing yeast in liquid medium].

Growth of white rot fungus in liquid medium with different trace element and effect of these medium on suppressing the growth of yeast under non-sterile were investigated in agitated Erlenmeyer flasks. Results showed that the size and amount of mycelium pellets in liquid medium with trace element and Fe 3.5 mg/L was more than that with trace element but no iron and without any trace element under sterile condition; moreover, the size and amount of mycelium pellets increased with the rise of the concentration of trace element and iron. In addition, if applying the mode of incubating under sterile condition and degrading under non-sterile condition, the decolorization of reactive brilliant red K-2BP in liquid medium without any trace element and with trace element but no iron was similar to that of degrading under sterile condition; but the decolorization in liquid medium with trace element and Fe 3.5 mg/L was less than the results under sterile condition. Through observing under microscope, it was found that the amount of yeast in liquid medium with Fe 3.5 mg/L was more than the medium without any trace element and with trace element but no iron. Therefore, trace element, especially iron, plays a very important role in suppressing yeast in liquid medium; furthermore, iron also is necessary to the growth of whit rot fungus. So that, to control the concentration of iron in liquid medium could reduce the invasion of yeast under non-sterile condition as long as it does influence the growth of white rot fungus.

Azo Compounds↗

PubMed2005

Detection of germline mutations of hMLH1 and hMSH2 based on cDNA sequencing in China.

AIM: To detect the germline mutations of hMLH1 and hMSH2 based on mRNA sequencing to identify hereditary non-polyposis colorectal cancer (HNPCC) families. METHODS: Total RNA was extracted from peripheral blood of 14 members from 12 different families fulfilling Amsterdam criteria II. mRNA of hMLH1 and hMSH2 was reversed with special primers and heat-resistant reverse transcriptase. cDNA was amplified with expand long template PCR and cDNA sequencing analysis was followed. RESULT: Seven germline mutations were found in 6 families (6/12, 50%), in 4 hMLH1 and 3 hMSH2 mutations (4/12, 33.3%); (3/12, 25%). The mutation types involved 4 missense, 1 silent and 1 frame shift mutations as well as 1 mutation in the non-coding area. Four out of the seven mutations have not been reported previously. The 4 hMLH1 mutations were distributed in exons 8, 12, 16, and 19. The 3 hMSH2 mutations were distributed in exons 1 and 2. Six out of the 7 mutations were pathological, which were distributed in 5 HNPCC families. CONCLUSION: Germline mutations of hMLH1 and hMSH2 can be found based on cDNA sequencing so as to identify HNPCC family, which is highly sensitive and has the advantages of cost and time saving.

Adaptor Proteins, Signal Transducing↗

PubMed2005

Point mutation of 5' noncoding region of BCL-6 gene in primary gastric lymphomas.

AIM: To investigate the mutations of the 5' noncoding region of BCL-6 gene in Chinese patients with primary gastric lymphomas. METHODS: PCR and direct DNA sequencing were used to identify BCL-6 gene mutations in the 5' noncoding region in 29 cases of gastric diffuse large B-cell lymphoma (DLBCL) and 18 cases of gastric mucosa-associated lymphoid tissue (MALT) lymphoma as well as 10 cases of reactive hyperplasia of lymph node (LRH). RESULTS: Six of 29 gastric DLBCLs (20.7%), 4 of 18 gastric MALT lymphomas (22.2%) and 1 of 10 LRHs(10%) were found to have mutations. All mutations were single-base substitutions and the frequency of single-base changes was 0.20 x 10(-2) -1.02 x 10(-2) per bp. CONCLUSION: Point mutations in the 5' noncoding region of BCL-6 gene are found in Chinese patients with primary gastric DLBCLs and MALT lymphomas, suggesting that they may, in some extent, participate in the pathogenesis of primary gastric DLBCLs and MALT lymphomas.

5' Untranslated Regions↗

PubMed2004

[Study of mutations in 5'-noncoding region of BCL-6 gene in B-cell non-Hodgkin's lymphomas].

OBJECTIVE: To investigate BCL-6 gene mutations in B-cell non-Hodgkin lymphomas (B-NHL) and their implications in lymphoma pathogenesis. METHODS: Polymerase chain reaction (PCR) and direct DNA sequencing methods were used to identify mutations in the 5'-noncoding region of BCL-6 gene in 135 cases of B-NHL, 5 cases of T-NHL, 5 cases of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and 10 cases of reactive hyperplasia of lymph node. RESULTS: Mutations were identified in 6 cases of nodal DLBCL (27.3%), 4 cases of FL (22.2%), 4 cases of MALT lymphoma (22.2%), 4 cases of extranodal DLBCL (20.7%) and 2 cases of LRH (20%). No mutations were detected in T-NHL and NLPHL (P < 0.05). There were no significant differences in incidences of BCL-6 gene mutations between nodal and extranodal DLBCL (P > 0.05). All mutations were base substitutions and the frequency of single-base change was 0.14 x 10(-2)/bp approximately 0.68 x 10(-2)/bp. CONCLUSIONS: Mutations of the 5'non-coding region of BCL-6 gene may be involved in the pathogenesis and progression of B-NHL. Molecular demonstration of such mutations may provide a marker of lymphomas derived from the germinal center-related B cells.

5' Untranslated Regions↗