[Nested coamplification polymerase chain reaction].
OBJECTIVE: To develop a simple and reliable method for intensifying the hybridization signals of gene chips. METHODS: The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip. RESULTS: With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater. CONCLUSION: Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.
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