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PubMed · 12170477

[Nested coamplification polymerase chain reaction].

Qingjie Xia·Sizhong Zhang·Yuan Yang·Jun Wang·Xiujie Wang·Yongxin Ma·Yan Sun
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Abstract

OBJECTIVE: To develop a simple and reliable method for intensifying the hybridization signals of gene chips. METHODS: The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip. RESULTS: With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater. CONCLUSION: Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.

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Base SequenceDNA ProbesEdetic AcidFluorescent DyesMicroscopy, FluorescenceMolecular Sequence DataNucleic Acid HybridizationPolymerase Chain ReactionReproducibility of ResultsEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Qingjie Xia, Sizhong Zhang, Yuan Yang, Jun Wang, Xiujie Wang, Yongxin Ma, Yan Sun. 2002. [Nested coamplification polymerase chain reaction].. https://pubmed.ncbi.nlm.nih.gov/12170477/

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