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Y C Ling

Publications and source records attributed to Y C Ling.

7 recordsLinked to original sources
PubMed1989

Quantitative imaging of boron, calcium, magnesium, potassium, and sodium distributions in cultured cells with ion microscopy.

A method for the conversion of intensity information in ion micrographs of freeze-fractured, freeze-dried cultured cells to local dry weight elemental concentrations is presented. Homogenates generated from cultured cells are used as calibration standards. Ion microscope (IM) relative sensitivity factors for B, Ca, K, Mg, and Na with respect to the matrix element C are determined by the correlation of IM and inductively coupled plasma atomic emission spectrometry analyses of the cellular homogenates. After calibration of the IM imaging system, the relative sensitivity factors are used to determine local intracellular concentrations of B, Ca, K, Mg, and Na in cultured Swiss 3T3 fibroblasts. Intracellular B was introduced through cellular uptake of Na2B12H11SH, a candidate therapeutic agent for boron neutron capture cancer therapy. The IM intracellular concentration results show good agreement with published electron probe X-ray microanalysis results. Estimated detection limits are in the low- to subparts-per-million dry weight concentration range.

Animals↗
PubMed1989

Quantitative imaging of free and total intracellular calcium in cultured cells.

Techniques of fluorescence and ion microscopies were combined to study the free [Ca2+] and total Ca in NIH 3T3 fibroblast and L6 rat myoblast cells. Free Ca2+ measurements with the Ca2+ indicator fura-2 and digital imaging reveal an inhomogeneous distribution of free cytoplasmic Ca2+ in both cell lines. Fura-2 also reveals a difference in free Ca2+ activity between the nucleus and cytoplasm of cells. Ion microscopic observations on sister cells show that total Ca in the cytoplasm is also inhomogeneously distributed and that mean cytoplasmic levels of total Ca are higher than levels in the nuclei. In the nuclei of NIH 3T3 cells, the mean free [Ca2+] and total [Ca] were 110 +/- 30 nM and 225 +/- 43 microM, respectively, while regions in the cell cytoplasm contained up to 490 +/- 270 nM free [Ca2+] and 559 +/- 184 microM mean total [Ca]. Intracellular total Ca was greater than 3 orders of magnitude higher than intracellular free Ca2+ in either nuclear or cytoplasmic compartments. Perinuclear cytoplasmic regions in 3T3 cells contained higher free and total Ca than the cell nucleus. Loading of cells with fura-2 did not modify the subcellular distribution of total K, Na, Ca, or Mg. This combination of two powerful ion imaging techniques provides a comparison between free and total calcium in cells and introduces a different approach for examining the role of this important element in cell physiology.

Animals↗
PubMed1989

Experience using microvascular anastomotic rings for the transplantation of 20 free flaps.

A new microvascular anastomosis technique was employed with success in 1985-1986 with the result of 18 free-flap survivals and 2 failures. The rate of success in microvascular anastomoses was 90.5 percent (38 of 42). The advantages of this technique are its simplicity, convenience, and safety, and it may shorten the duration of the operation. Our experience in using vascular anastomotic rings is presented.

Adult↗
PubMed1987

SIMIPS: secondary ion mass image processing system.

A secondary ion mass image processing system (SIMIPS) is presented as a quantitative image analysis tool, with emphasis on an efficient man-machine interface. The combined applications of digital image processing and pattern recognition ensure an intelligent problem-resolving scheme and optimal extraction of information. The system performance is evaluated, and typical applications are presented to illustrate the versatility and usefulness of SIMIPS in analyzing digital images.

Colon↗
PubMedDate not supplied

Clinical experience and surgical anatomy of 32 free anterolateral thigh flap transplantations.

The anterolateral thigh flap, first described by Song, is one of the septocutaneous flaps in the thigh. Since November 1985 we have transferred 32 anterolateral thigh flaps successfully without any loss. Six of these flaps were performed in Australia. The vascular pedicles are divided into three types: fasciocutaneous perforator (37.5%-12/32), vertical musculocutaneous perforator (56.2%-18/32) and horizontal musculocutaneous perforator (6.6%-2/32). This paper presents the surgical anatomy of the flap and clinical experience.

Adolescent↗