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Biomedical subjects

Y F Shi

Publications and source records attributed to Y F Shi.

At least 91 records · Page 5Linked to original sources
PubMed1984

Long-term stability of aqueous solutions of luteinizing hormone-releasing hormone assessed by an in vitro bioassay and liquid chromatography.

The stability of aqueous solutions of luteinizing hormone-releasing hormone (LHRH) after extended storage at various temperatures was investigated using a newly developed HPLC assay and an in vitro dispersed pituitary cell culture bioassay. Good correlations were obtained between the potency obtained by HPLC and bioassay in samples stored at 37 degrees C or subjected to different stress conditions. No significant decrease in activity of LHRH was observed in aqueous solutions stored at 37 degrees C for up to 10 weeks, at 4 degrees C for 2 years, or subjected to repeated freezing and thawing for 5 d. Heating to 60 degrees C in sterile pH 9.0 buffer up to 11 d and storage at ambient temperature in nonsterile solution for 4 months produced well-distinguished degradation products and a decrease in potency. It is concluded that sterile aqueous solutions of LHRH are stable for at least 10 weeks at 37 degrees C and, thus, could be reliably used for chronic administration when long-term stability at body temperature is important.

Animals↗
PubMed1984

Observations of Langerhans' cells in leprosy using monoclonal antibody OKT6.

Changes in the Langerhans' cells (LC) in seven cases of leprosy were observed using the monoclonal antibody OKT6. In the epidermis of the lesions of most of the leprosy cases, the processes of LC were reduced or diminished. Some LC were disintegrated. In two BT cases in the upgrading (type 1) reactional stage, either increased numbers and aggregation of OKT6-positive cells in clusters or their disintegration was seen in different areas of the epidermis.

Adult↗
PubMed1982

Preliminary observation on Langerhan's cells in leprosy.

Langerhans' cells were enumerated in the skin of eight borderline and tuberculoid leprosy patients by the enzymatic histochemical demonstration of ATPase in these cells in epidermal sheets. The number of Langerhans' cells was significantly reduced in the skin lesions of these patients compared with normal appearing skin from the same patients. The morphological details of these cells were not clear under light microscopy; further study with electron microscopy is desirable.

Adolescent↗