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Biomedical subjects

Y Hisa

Publications and source records attributed to Y Hisa.

61 records · Page 4Linked to original sources
PubMedDate not supplied

Postnatal ontogeny of catecholamine and somatostatin neuron systems in the median eminence of the rat as revealed by a colocalization technique.

The ontogenetic development of catecholamine (CA) and somatostatin containing nerve terminals in the rostral, central and caudal median eminence (ME) of the rat was investigated by combining fluorescence histochemistry and immunohistochemistry in the same tissue section. Somatostatin terminals were detected earlier in development than CA terminals and had already appeared in the lateral part of the external layer of the central ME by the 1st postnatal day. CA nerve terminals were first observed in the same region of the ME on the 7th postnatal day. At about this stage both types of terminals seemed to show early signs of a correlation in their distribution which became progressively closer as maturation proceeded. Their distribution reached a stable condition in density and pattern on the 21st postnatal day when the majority were found in the lateral part of the external layer of the central ME, in the basal part of the brain just dorsal to the tuberoinfundibular sulcus and in the upper and lower labia of the tuberoinfundibular stalk. They subsequently matured to the adult pattern of stabilized distribution and anatomical relationship. It was also established that on the 28th postnatal day the somatostatin immunoreactive terminals reached a greater concentration than at any other stage of development including that of the adult.

Aging↗
PubMedDate not supplied

Ultrastructural and fluorescence histochemical studies on the sympathetic innervation of the canine laryngeal glands.

The existence of the adrenergic terminals of the canine laryngeal glands has been revealed by electron microscopy and fluorescence histochemistry. Adrenergic fibres with fluorescent varicosities were observed around the base of th acini in the submucosa. In dogs treated with 5-OHDA, varicosities containing both small and large, dense-cored vesicles containing both small and large, dense-cored vesicles, believed to be adrenergic terminals, were found near blood vessels, gland cells and myoepithelial cells in the submucosal gland region. The role of these adrenergic terminals is briefly discussed.

Adrenergic Fibers↗
PubMedDate not supplied

Substance P nerve fibres in the canine larynx by PAP immunohistochemistry.

The distribution of the Substance P (SP) immunoreactive nerve fibres in the canine larynx and laryngeal nerves was studied by PAP immunohistochemistry. Many individual SP immunoreactive nerve fibres with varicosities were observed within the epithelial layer and in the connective tissue below the epithelium of the laryngeal mucosa. Small numbers of SP immunoreactive nerve fibres were also found in the submucosal gland region and some of them appeared to terminate in glandular cells. These findings are consistent with the view that SP might be involved in the laryngeal sensory innervation system and the laryngeal glandular secretion. No SP immunoreactive nerve fibres were found in any intrinsic laryngeal muscles. The recurrent laryngeal nerve and the superior laryngeal nerve contained SP immunoreactive nerve fibres and were considered to lie in the pathway of the SP nerve fibres to the larynx.

Animals↗