Beneficial impact of FK 409 as donor pretreatment on warm or cold ischemically injured hearts in the rat.
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Animals↗
Biomedical subjects
Y Hui
Publications and source records attributed to Y Hui.
Non-breath-hold gadolinium-enhanced MR angiography of the thoracoabdominal aorta: experience in 18 children.
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Adolescent↗
Intrahepatic peripheral cholangiocarcinoma: CT features in 18 pathologically proven cases.
OBJECTIVE: To determine the morphological features of 18 pathologically proven intrahepatic peripheral cholangiocarcinoma (IHPCC) cases in computerized tomography (CT) image. METHODS: All 18 patients had CT, using Picker I.Q.T/C and taking pre-contrast continuous 10-mm sections throughout the liver and post-contrast continuous 10-mm sections throughout the focuses. RESULTS: The disease was characterized in CT image by the following: all focuses were found in the periphery of the liver and shown as a lobulated or fused hypodense space-occupying mass; there were one or more divergent or confluent, circular or irregular cystic areas with much lower density, in the majority. All focuses could be enhanced slightly and most revealed a dim edge. Dilated bile ducts around the focus were found frequently; the dilated bile ducts especially seemed to encircle the focus (33.3%, 6/18). This phenomenon were referred to as "encysted sign of dilated bile ducts". CONCLUSIONS: CT scanning should be one of the most important investigative methods for IHPCC due to the disease characteristics identified in CT image, especially "encysted sign of dilated bile ducts" which possesses specificity in diagnosing the disease.
Adult↗
[Cell density and expression of proliferating cell nuclear antigen (PCNA) in the lens epithelium of children and aging patients with cataract].
OBJECTIVE: To determine the cell density and expression of proliferating cell nuclear antigen (PCNA) in the lens epithelium of children and aging patients with cataract. METHODS: The lens epithelial cells (LECs) of the central area were examined in two groups, children (less than 12 years old) and senile persons (51 - 80 years old). The densities of LECs in the two groups were calculated with samples stained with hematoxylin and eosin (HE) staining, and the PCNA was tested immunohistochemically. The integral optical density (IOD) of PCNA expression was measured through a medical color image analysis system. RESULTS: The densities of LECs in children and senile persons were 5,020.25 +/- 246.01 cells/mm(2) and 4,340.00 +/- 240.95 cells/mm(2) (P < 0.05), the IODs were 2.89 +/- 0.57 and 2.13 +/- 0.63 (P < 0.05) respectively. CONCLUSION: The LEC counting and IOD values in children eyes are significantly higher than that of senile persons, suggesting that they be one of the important factors in the formation of posterior capsular opacification (PCO) or after cataract in children eyes.
Aged↗
[Precollagen III in the vitreous of eyes with proliferative vitreoretinopathy].
PURPOSE: To detect precollagen III (PC III) levels in serum and vitreous samples from patients with proliferative vitreoretinopathy (PVR). METHODS: PC III levels were measured by radioimmunoassay in 5 cadaveric vitreous samples from normal subjects, 20 normal human serum samples, 29 vitreous and serum samples from patients with rhegmatogenous retinal detachment complicated with PVR. The relationship between PC III levels and PVR history, surgery, epiretinal membrane, severity of PVR were analyzed with Logistic regression analysis. RESULTS: The mean PC III levels in serum samples of PVR patients and control subjects were 83.76 +/- 18.52 and 85.02 +/- 17.50 micrograms/L respectively (P > 0.05). PC III was not detected (< 40 micrograms/L) in the cadaveric vitreous. In vitreous aspirates from 29 eyes with PVR, it was not detected in 12 eyes, but higher than 40 micrograms/L in 17 eyes with the mean level of 268.69 +/- 176.07 micrograms/L. (P < 0.05) The high levels correlated with duration and severity of PVR. As onset time and PVR grade increasing, the probability of PC III concentration higher than 40 micrograms/L was 5.2655 and 2.7978 times the concentration lower than 40 micrograms/L respectively. CONCLUSION: PC III can be detected in vitreous aspirates from PVR eyes. The PC III levels in the vitreous was an event of local responses and were correlated with severity and history of PVR. This suggests that PC III and collagen III be involved in the development of PVR.
Adolescent↗
Inhibition effect of cyclosporin A on human retinal pigment epithelial cells in vitro.
PURPOSE: To select effective drugs against cellular proliferation in the vitreous. METHODS: Cyclosporin A (0.125 mg/l-4.0 mg/l) was added to cultures of human retinal pigment epithelial (RPE) cells with or without macrophage-conditioned medium (MCM). Proliferation rate of the cells was measured with (3H)-thymidine incorporation and liquid scintillation techniques on days 3 and 5. RESULTS: Cyclosporin A at a dosages of 0.125 mg/l had a slight inhibition on human RPE cells proliferation (-3.8%-4.1%, P > 0.05). Cyclosporin A at a dose ranging from 0.25 mg/l to 4.0 mg/l inhibited cellular proliferation effectively and in a dose-dependent manner (8.7%-95.1%, P < 0.05 r = 0.94, P < 0.001) with its ID50 of 1.49 mg/l. In the culture of RPE with MCM, the inhibition on day 5 was more effective than that on day 3. CONCLUSION: Cyclosporin A had an effective inhibition on human RPE cell proliferation and it may be of potential use clinically.
Adult↗
Extranasopharyngeal angiofibroma of the inferior turbinate.
The first reported case of angiofibroma of an inferior turbinate is presented. The tumour occurred in a 9-year-old boy and was extirpated by subperiosteal dissection of the lateral nasal wall.
Angiofibroma↗
[Expression of IL-4 and IL-10 by PBMC and tumor tissues in cancer patients].
Using biological activity assay, the production of IL-4 and IL-10 by peripheral blood mononuclear cells (PBMC) was studied in patients with nasopharyngeal carcinoma and lymphoma. The IL-2, IL-4 and IFN gamma levels in both cancer patients were lower than that in normal subjects. In contrast, the IL-10 level was higher in both cancer patients. When rIL-2 was added into the cytokine production system, IL-2, IL-4, and IFN gamma level were upregulated in lymphoma patients. However, in the patients of nasopharyneal carcinoma, only IL-2 level was upregulated. IL-10 activity could not be affected by exogenous IL-2 in both cancer patients. The results of negative IL-4 activity and higher positive percentage (84.6%) of IL-10 activity in ascites from patients with ovarian carcinoma were corresponded to the patients with nasopharyngeal carcinoma and lymphoma. By using in situ hybridization technique, the expression of IL-4 and IL-10 was studied in tumor tissues from patients with gastric, esophageal and breast carcinomas. There was no differences of the percentage of IL-4 and IL-10 expression among three kinds of tumor tissues, the range of positive percentage was 40%-70%. These results indicate that the correction of abnormal upregulation of IL-10 activity should be considered for cancer immunotherapy.
Breast Neoplasms↗
[Antitumor effect on melanoma by in vivo direct injection of packaging cells transfected recombinant IL-2 gene].
Our previous studies showed that ex vivo transfer of human IL-2 gene into murine B16 melanoma cells resulted in the expression of IL-2 activity and had inhibitory effect on tumor growth in vivo. In this study, B16 melanoma bearing mice were injected directly with IL-2 recombinant PA317 cells. The in vivo gene transfer revealed significant reduction of tumor size and prolongation of survival of tumor bearing mice. Using slot blot analysis, the expression of IL-2 mRNA in tumor mass was detected and the IFN gamma activity was also enhanced in gene transferred mice. These results indicated that in vivo direct transfer of IL-2 recombinant retrovirual vector producing cells would induce transfer gene into tumor cells and exert its antitumor effect.
Animals↗
Vitrectomy for complicated Eales disease.
PURPOSE: We determined the efficacy of pars plana vitrectomy in a series of patients with complicated Eales disease. METHODS: Clinical records were reviewed on 47 consecutive patients (49 eyes) who underwent vitrectomy for persistent vitreous hemorrhage (PVH) and/or traction retinal detachment (TRD) with neovascular membranes (NVM). All cases were divided into 4 subgroups of eyes with PVH (without obvious NVM, n = 9), NVD (NVM from the optic disc, n = 10), NVP (peripheral NVM, n = 11), or ENV (extensive NVM and logn-standing TRD, n = 17). RESULTS: The study included 8 women and 39 men with a mean age of 27.3 years (range, 16 to 50 years). The visual acuity was light perception in 15 eyes, hand motions in 18 eyes, counting fingers in 6 eyes and 0.02 to 0.1 in 10 eyes before vitrectomy. Visual acuity improved postoperatively in all eyes with PVH, NVD of NVP with a mean visual acuity of 0.32 (range, 0.05 to 1.0, n = 28) except 3 eyes unchanged. There was no significant difference (P > 0.05) in the patient age, preoperative visual acuity, or visual improvement after vitrectomy among these 3 subgroups. In the eyes with ENV, however, postoperative visual improvement was obtained but limited in 9 eyes (range, 0.02 to 0.15). Visual acuity was unchanged in 9 eyes. There was a significant difference (P < 0.01) in postoperative visual acuity between the eyes with ENV and the eyes with PVH, NVD or NVP. CONCLUSIONS: Vitrectomy with removal of persistent vitreous hemorrhage and focal neovascular membranes and endolaser photocoagulation may lead to improved visual acuity in the majority of eyes with Eales disease. However, extensive fibrovascular membranes and long-standing retinal detachment result in poor visual outcome. Earlier surgery can be considered to prevent visual loss in the patients.
Adolescent↗
[IL-8 in experimental proliferative vitreoretiopathy induced by macrophages].
PURPOSE: To measure the concentration changes of IL-8 during the development of experimental proliferative vitreoretiopathy (PVR) induecd by macrophages and explore the effect on the inflammatory cells at the early stage of the model. METHODS: Rabbit PVR model was induced by homogenous macrophages. The vitreous, agueous and vein blood were taken at different periods and IL-8 levels in them were measured with an ELISA kit. RESULTS: The IL-8 level reached its peak of 1,325 pg/ml at 14th day in the model which was 14 times as the level of controls. The level then decreased to 48 pg/ml at 28th day after macrophage injection. CONCLUSION: The changes of IL-8 in the model were parallel to the inflammatory reaction during the development of PVR, suggesting that IL-8 may play an important role the inflammatory cells' infiltrating and migrating to the vitreous.
Animals↗
[ECG changes during [correction of dring] high G centrifuge training].
ECG of 47 healthy subjects, during G-training on the human centrifuge were recorded and analyzed. Training consisted of 4 G profiles and carried out in 6 d. Subjects were divided into 3 groups according to different anti-G equipment used. The results showed that: high incidence of arrhythmia (87%) was observed, and it increased with the increase of G level; subjects with R/S < 1 under +Gz environment, or HR sharply dropped immediately after the +Gz load have lower G-tolerance; incidence of arrhythmia is lower when advanced anti-G equipment was used.
Acceleration↗
Ototoxicity from ototopical aminoglycosides.
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Administration, Topical↗
Improving American Indian Cancer Data in the Washington State Cancer Registry using linkages with the Indian Health Service and Tribal Records.
BACKGROUND: Previous reports have suggested that American Indians are sometimes classified as other races on cancer registries. Also, cancer registries typically do not include data on tribal affiliation. This study determined the extent of racial misclassification of American Indians in the Washington State Cancer Registry (WSCR) and obtained tribal-specific cancer data for Washington State. METHODS: A computer file including persons registered for services with the Portland Area Indian Health Service (IHS) or who were enrolled members of 19 tribes in Washington, Oregon, and Idaho (n = 127,375) was linked with WSCR records of incident cases for 1992 and 1993 (n = 49,420). Linkage was conducted with probabilistic methods using the AutoMatch software package. RESULTS: Of 180 persons recorded as American Indian in the WSCR, 130 (72.2%) were identified in the IHS/tribal roll file. Of 259 American Indians included in the IHS/tribal file who were identified in the WSCR, 130 (50.2%) were classified as American Indian. The estimated age-adjusted cancer incidence among American Indians in Washington State increased from 153.5 per 100,000 population before record linkage to 267.5 per 100,000 after linkage. Of the 259 persons who were linked to the WSCR, 17 were not registered with IHS and appeared solely in the tribal rolls. Only two tribes had more than five identified cancer cases during the 2-year study period. CONCLUSIONS: The number of IHS-enrolled American Indians or tribal members included in the WSCR would be underestimated by one third in the absence of record linkages, and the estimated cancer incidence of 43.6% would be lower before linkage. It is feasible to obtain tribal-specific cancer rates by linking tribal rolls to cancer registries, although the small number of cases in most tribes is a significant limitation. Further efforts to improve racial classification of American Indians in cancer registries should be undertaken.
Data Collection↗
Synthesis and structure-activity relationships of 2-pyridones: a novel series of potent DNA gyrase inhibitors as antibacterial agents.
Two novel series of 2-pyridones were synthesized by transposition of the nitrogen of 4-quinolones to the bridgehead position. This subtle interchange of the nitrogen atom with a carbon atom yielded two novel heterocyclic nuclei, pyrido[1,2-alpha]pyrimidine and quinolizine, which had not previously been evaluated as antibacterial agents and were found to be potent inhibitors of DNA gyrase. Quinolizines with a methyl group at the 9-position such as (S)-45a (ABT-719) demonstrate exceptional broad spectrum antibacterial activity. Most notably, they are active against resistant bacteria such as methicillin-resistant Staphylococcus aureus, vancomycin-resistant strains of enterococci, and ciprofloxacin-resistant organisms. In addition, 2-pyridones also possess favorable physiochemical and pharmacokinetic properties. These 2-pyridones were synthesized from the commercially available starting materials by 10-17 linear transformations. The structure of an adduct yielded by this sequence, (S)-45a (ABT-719), was determined by X-ray crystallographic analysis.
Animals↗
Risk factors of tracheostomal recurrence after laryngectomy for laryngeal carcinoma.
BACKGROUND: Tracheostomal recurrence after total laryngectomy for laryngeal carcinoma has a poor prognosis. The independent risk factors of tracheostomal recurrence are, however, not well documented. METHODS: This is a multivariate analysis of the risk factors of tracheostomal recurrence after total laryngectomy for 322 laryngeal squamous cell carcinomas. The factors included in the analysis were sex, age, tumor stage, sites of tumor involvement, preoperative airway obstruction, preoperative tracheostomy, extent of surgical resection, radiotherapy, and pathological resection margin. RESULTS: Seventeen (5%) patients developed tracheostomal recurrence. Univariate analysis showed that preoperative airway obstruction, subglottic involvement, and postcricoid extension were significant factors associated with tracheostomal recurrence. Multivariate analysis using logistic regression method showed that both subglottic and postcricoid involvement were independent predisposing factors for tracheostomal recurrence. The tracheostomal recurrence rates were 2% in patients without the risk factor and 10% in patients with the presence of one or both risk factors. CONCLUSIONS: Subglottic and postcricoid involvement were independent risk factors for tracheostomal recurrence.
Adult↗
Primary closure of pharyngeal remnant after total laryngectomy and partial pharyngectomy: how much residual mucosa is sufficient?
After total laryngectomy with or without partial pharyngectomy, the remaining pharyngeal defect can be repaired either by primary closure or with additional tissue, depending on the amount of pharyngeal tissue remnant available. The aim of this study was to determine the minimum width of the pharyngeal remnant that could be safely closed primarily without causing difficulty in swallowing. A total of 52 consecutive patients who underwent total laryngectomy were entered into the study. The relaxed and stretched widths of the pharyngeal remnant were measured after removal of the specimen. The widths of the pharyngeal mucosa ranged from 1.5 to 5.0 cm relaxed (mean, 3.24 cm) and from 2.5 to 8.0 cm stretched (mean, 4.83 cm). All neopharynx was reconstructed by closing the pharynx primarily. Seven of the 52 patients developed recurrent tumor with concomitant dysphagia. Two of the 45 patients without recurrence presented with acute dysphagia from food bolus obstruction, and 1 patient developed benign inflammatory stricture following an episode of fish-bone impaction. The narrowest widths of the pharyngeal remnant in this group of 45 were 1.5 cm relaxed and 2.5 cm stretched. As these patients do not have swallowing difficulty, we conclude that in the absence of tumor recurrence, this amount of residual pharyngeal tissue is sufficient both for primary closure of the pharynx and in restoring swallowing function.
Adult↗
[Promotion of c-fos oncogene expression in cultured rabbit lens epithelial cells by macrophages].
OBJECTIVE: This study was designed to test the hypothesis that inflammatory cells deposited on the surface of implanted intraocular lens stimulate the proliferation of residual lens epithelial cells. METHODS: Immunocytochemical ABC methods with purified anti-human Fos protein serum antibody was used to stain the cultured rabbit lens epithelial cells incubated with rabbit macrophages and macrophage-conditioned medium (MCM). RESULTS: The lens epithelial cell growth was faster in the cultures with macrophages and MCM than in the controls. Fos protein positive staining was found in the nuclei of epithelial cells cultured with macrophages for 4 hours, and of the cells with MCM for one hour; whereas the staining was negative in the cells of control cultures. CONCLUSION: Macrophages promote c-fos oncogene expression in the lens epithelial cells, that might be mediated by bioactive factors secreted by macrophages.
Animals↗