PubMed Health⌕ Search

Biomedical subjects

Y Hui

Publications and source records attributed to Y Hui.

At least 91 records · Page 5Linked to original sources
PubMed1996

Relationship between some humoral factors and left ventricular hypertrophy in essential hypertension.

OBJECTIVE: To evaluate the relationship between certain humoral factors and left ventricular hypertrophy (LVH) in essential hypertension. PATIENTS AND METHODS: 62 essential hypertension (EH) patients (32 men, 30 women; mean age, 55 years) and 20 normotensive healthy subjects (10 men, 10 women: mean age, 52 years) were studied. EH patients were divided into LVH group and non-LVH group by echocardiography. After an overnight fast, blood samples were taken for the determination of parathyroid hormone (PTH), angiotensin II (ATII) and aldosterone (ALD) by radioimmunoassay. RESULTS: There was a significant difference in PTH. AT II and ALD between the EH group and control group. Furthermore, in LVH group PTH, ATII and ALD elevated significantly as compared with non-LVH group. In addition, we found that LVMI (left ventricular mass index) correlated with ATII (r = 0.342, P < 0.01) and ALD (r = 0.356, P < 0.01). There was a more significant correlation between LVMI and PTH (r = 0.422, P < 0.0025). CONCLUSIONS: Some humoral factors are important determinants of LV mass. Besides the reninangiotensin-aldosterone system, PTH might play an important role in cardiac hypertrophy.

Aldosterone↗
PubMed1996

An immunohistochemical study of IgG, complement C3, collagen type III and macrophage-marker Ki-M7 in epiretinal membranes.

PURPOSE: To observe the immunological changes in epiretinal membranes from the patients with proliferative vitreoretinopathy (PVR). METHODS: Twelve samples of epiretinal membranes obtained during vitreous surgeries for PVR were examined by direct and indirect immunofluorescein histochemistry. RESULTS: The cellular membranes in 8 cases were composed of retinal pigment epithelial cells, fibroblast-like cells, macrophages and collagen. Positive stainings of IgG, C3+ collagen type III and macrophage-marker Ki-M7 (CD68) was seen in the membranes. CONCLUSION: The results indicate that humoral immune components and macrophages may play an important role in the development of epiretinal membrane formation.

Adolescent↗
PubMed1996

[Exploratory vitrectomy for traumatized eyes with no light perception and dense vitreous hemorrhage].

OBJECTIVE: To evaluate the efficacy of exploratory vitrectomy for certain traumatized eyes with no light perception (NLP) and dense vitreous hemorrhage. METHOD: We performed vitrectomy on 10 patients with NLP eyes after ocular trauma. Of them, eight had open globe injuries and two had closed blunt ocular trauma. RESULTS: Postoperatively, six had visual acuities of light perception to 0.25 and four remained NLP. In most eyes, a potentially functional posterior retina and optic disc were observed during surgery. CONCLUSION: Dense vitreous hemorrhage could be the most important factor for NLP after severe ocular trauma. Therefore, NLP does not always mean permanent loss of vision. Proper primary repair of the wound and timely vitrectomy may give a chance of visual recovery in selected traumatized eyes.

Adolescent↗
PubMed1995

Sinusitis in patients with cystic fibrosis.

The management of sinus disease in children with cystic fibrosis is reviewed, based on a literature review and clinical experience in The Hospital for Sick Children, Toronto. Diagnostic and treatment approaches are discussed, with emphasis given to indications for surgical therapies. Great importance is given to the preservation of normal nasal anatomy whenever possible to minimize the possibility of iatrogenic injuries occurring, especially when revision surgery is required.

Adolescent↗
PubMed1995

Congenital nasal pyriform aperture stenosis as a presenting feature of holoprosencephaly.

Congenital nasal pyriform apertures stenosis (CNPAS), a recently recognized uncommon cause of nasal airway obstruction, can be a life-threatening circumstance in the neonate. This study's experience with six cases confirmed the suggestion that CNPAS represents a manifestation of holoprosencephaly. Management of this condition depends firstly on the overall prognosis of the patient and secondly the severity of obstruction. Patients with poor overall prognosis should be managed conservatively with an oropharyngeal airway. In patients with a good outlook, the choice of treatment is conservative for those with less severe obstruction and surgical correction for those with complete obstruction. The ability to pass a No. 5Fr catheter (O.D. 1.67 mm) may be a guide to the choice of treatment. An increase in awareness among otolaryngologists on the nature of CNPAS is necessary to improve overall management of such patients.

Child↗
PubMed1995

Sensitive detection of selected cholinergic channel activators derivatized with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole.

A high-performance liquid chromatographic method with fluorescence detection was developed for a series of cholinergic channel activators using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) as a fluorescent labeling reagent. This method includes three separate steps: extraction of parent compound and internal standard with organic solvent from plasma, reaction of parent and internal standard in NBD-F precolumn to yield a fluorescent product and extraction of the resultant fluorophores with organic solvents. The extraction and reaction procedures were optimized for this series of structurally new compounds. The method showed high sensitivity, selectivity and reproducibility and proved useful for the determination of ng ml-1 plasma levels of selected cholinergic channel activators.

4-Chloro-7-nitrobenzofurazan↗
PubMed1995

Laser aryepiglottoplasty for the treatment of neurasthenic laryngomalacia in cerebral palsy.

Patients with cerebral palsy usually suffer from lack of coordination in the neuromuscular mechanism in their upper airway and digestive tract. Difficulty in swallowing and aspiration are common problems in these patients, and stridorous breathing sometimes develops as a secondary symptom. Laryngoscopic examination revealed that redundant tissue in the aryepiglottic fold area was the cause of stridor and upper airway obstruction in four patients with cerebral palsy. We report on these four patients in whom laser reduction of the redundant mucosa led to dramatic improvements in stridor. Secondary benefits to family members and others were equally impressive.

Adolescent↗
PubMed1995

[Influence of radioimmunoimaging on the survival rate of ovarian carcinoma patients].

Two groups of patients suffered from ovarian carcinoma were studied retrospectively after similar treatments; in one group of 22 patients radioimmunoimaging (RII) had been used, which was not used in the other group of 15 patients. Other factors were similar in the two group such as hospitalization period, courses of surgical treatment and regimes of chemotherapy after surgery. The staging according to FIGO was also similar in the two groups except that the number of patients in stage III was 14 in the RII group but 8 in the non-RII group. Neither the age distribution nor the histopathological diagnosis was of much difference. 131I labeled monoclonal antibody against ovarian serous adenocarcinoma, generated in our laboratory, was given to all patients intraperitoneally either before or after the surgery; 7 cases before the initial operation, 9 cases before the second look operation and 6 cases for follow up after surgery and chemotherapy. The death rates of stage III were 42.9% and 75.0% in the RII group and non-RII group respectively. By means of Kaplain Meir's life table calculation the 3 year survival rates for the two groups were 63.5% and 25.0% respectively. The RII group was obviously higher than the non-RII group even though not statistically significant. These results were similar to those reported in the literature. Three very late cases of ovarian carcinoma were reported, in whom RII had been used, are still living after follow-up for more than 5 years. The beneficial role of induction of the idiotypic network was discussed, and the possibility of innoculation is under investigation.

Antibodies, Monoclonal↗
PubMed1995

[mRNA expression of vimentin gene in lens of transgenic mouse and DNA amplification in human cataract].

To investigate the role of vimentin gene in cataractogenesis, 12.7 Kb chicken vimentin genes were microinjected into the male pronuclei of 530 fertilized mouse eggs. 453 injected embryos were transferred into the oviducts of 18 pseudopregnant recipient females, of which 9 pregnant mice gave births to 37 offspring mice. The integration and expression of exogenous gene in the offsprings were analyzed by Southern and Northern blot hybridizations. It is shown that the E2 offspring was a transgenic mouse in which the chicken vimentin gene was integrated in its genome and efficiently expressed in mRNA level in its lens. In the human senile cataract lens, Bam-HI-restricted vimentin fragments displayed a stronger hybridization signal than that of the control lens in Southern blot analysis.

Adult↗
PubMed1995

Effect of perindopril and metoprolol on left ventricular hypertrophy and performance in essential hypertension.

The effects of perindopril and metoprolol on left ventricular hypertrophy (LVH) and function were studied in 47 essential hypertensive patients with LVH. Previous antihypertensive drugs were discontinued for at least 2 weeks, after which patients were randomly divided into 2 groups. 25 subjects were treated with perindopril 4 to 8 mg once daily in the morning (Group A) and 22 subjects with metoprolol 25 to 62.5 mg twice daily (Group B). The subjects were evaluated before and after 4 and 8 weeks of treatment by use of echocardiography. Before treatment LV mass indexes (LVMI) of two groups were respectively 143.2 +/- 21.3 g/m2 and 140.6 +/- 23.7 g/m2 (P > 0.05). In Group A, reduction of LVMI occurred after 4 weeks of treatment, and more pronounced after 8 weeks (from 143.2 +/- 21.3 g/m2 to 126.6 +/- 15.3 g/m2, P < 0.001), whereas reduction of LVMI occurred only after 8 weeks in Group B (from 140.6 +/- 23.7 g/m2 to 133.4 +/- 13.2 g/m2, P < 0.001). In addition, there was a significant (P < 0.05) difference in LVMI between the two groups after 8 weeks. LV systolic function remained unchanged, whereas E/A increased significantly (P < 0.001) in two groups after 8 weeks. In conclusion, antihypertensive treatment with perindopril and metoprolol induced a significant regression of LVH associated with improvement in LV diastolic performance. Perindopril, compared with metoprolol, was more effective in reversing LVH.

Adult↗
PubMed1995

[The influence of macrophage on the rates of cultured rabbit lens epithelial cell proliferation and DNA synthesis].

Cell and H3-TDR liquid scintillation countings were adopted to investigate the influence of macrophage on the proliferative rate of cultured rabbit lens epithelial cells, in vitro. After 24 hours of the action of macrophage opsonic fluid on the cell culture, the rates of cell proliferation and DNA synthesis in the experimental group were higher than those of the control group (P < 0.05) and maintained so to the fifth day. It is demonstrated that the macrophage and its active factors can promote the proliferation of the lens epithelial cells, that is possibly related to the posterior capsular opacification after extracapsular cataract extraction.

Animals↗
PubMed1995

mRNA expression of vimentin gene in lens of transgenic mouse and DNA amplification in human cataracts.

PURPOSE: To investigate the role of vimentin gene in cataractogenesis. METHODS: The 12.7kb chicken vimentin genes were microinjected into the male pronuclei of 918 fertilized mice eggs. 841 injected embryos were transferred into oviducts of pseudopregnant recipient females, of which 12 pregnant mice gave birth to 49 offspring mice. The integration and expression of exogenous gene in the offspring were analysed by Southern and Northern blot hybridizations. In the human senile cataract, the lens vimentin gene was analyzed with the chicken vimentin gene probe. RESULTS: It showed that four of F1 offspring were transgenic mice in which the chicken vimentin gene was integrated in their genomes. The transgenic band was 12kb, similar to the 12.7kb chicken vimentin fragment injected. One 2kb vimentin mRNA was visualized on E2 mouse lens blot, which revealed that the chicken vimentin gene was efficiently expressed in this transgenic mouse. In the human senile cataract lens, 12kb BamHI-restricted vimentin fragments displayed a stronger hybridization signal than that of the control lens in Southern blot analysis. It implies that the formation of human senile cataract may be associated with the amplification of vimentin gene. CONCLUSIONS: We have successfully developed four transgenic mice bearing chicken vimentin gene and having mRNA expression which can be used for further study. It is to be observed if the normal lens cell function is affected by the expressed product and cataract occurs in our transgenic mice. The cause of the gene amplification in human cataract remains for further investigation.

Adult↗
PubMed1995

[Lens epithelial proliferation induced by macrophages after intraocular lens implantation in rabbits].

To approach the effect of intraocular lens implantation on the mechanism of posterior capsular opacification, the pathological changes of posterior lens capsule after intraocular lens implantation in rabbits were observed by transmission electron microscopy. The results were as follows: In the control group, 1 week after the operation and the injection of the medium of the macrophage suspension, the lens posterior capsule was covered by a layer of lens epithelium and until post-operative 6 months, the lens epithelium remained normal. In the experimental group, 1 week after the operation and the injection of the macrophage suspension into the anterior chamber, microfilaments appeared in the cytoplasm of lens epithelial cells, after 2 weeks, the lens epithelial cells produced collagen fibrils in the extracellular matrix and until postoperative 3 months, 2-3 layers of the lens epithelium proliferated in front of the posterior capsule and a large amount of collagenous fibers were formed around the cells. It is suggested that the macrophage promote the lens epithelial proliferation and the posterior capsular opacification.

Animals↗
PubMed1994

[Amniotic fluid C-peptide in pregnancy with diabetes].

A prospective study on amniotic fluid C-peptide (AFCP) was made in 29 women with diabetic pregnancy and 23 non-diabetic control subjects. The mean concentrations of AFCP were found to be significantly higher in diabetic pregnancy than in non-diabetic pregnancy (P < 0.01) (diabetic group AFCP 1.89 +/- 1.53ng/ml; control group 0.88 +/- 0.51ng/ml). AFCP levels were related to the degree of blood glucose control during pregnancy and not to diabetic classification. The results show that fetal hyperinsulinemia reduced in these women that the achieved metabolic control of maternal blood glucose levels approached normal values. A higher neonatal complications occurred while AFCP was over 1.68ng/ml.

Amniotic Fluid↗
PubMed1994

Ofloxacin eardrop treatment for active chronic suppurative otitis media: prospective randomized study.

This report presents a prospective randomized study of 0.3 percent ofloxacin eardrops for the treatment of active chronic suppurative otitis media. Twenty-nine patients were treated with ofloxacin eardrops for 1 week, and another 27 patients were treated with Augmentin for 1 week. One week after completion of treatment, 22 (76%) patients of the group treated with ofloxacin had dry ears, and only seven (26%) patients treated with Augmentin had dry ears. Ofloxacin eardrops were effective in the initial treatment of active chronic suppurative otitis media.

Administration, Oral↗
PubMed1993

Crystallization and preliminary crystallographic data for formyltetrahydrofolate synthetase from Clostridium thermoaceticum.

Formyltetrahydrofolate synthetase from Clostridium thermoaceticum has been crystallized using the hanging-drop method from ammonium sulphate and PEG solutions. Crystals are trigonal, the space group is R32, a = 163 A, c = 259 A. A 4.2 A resolution data set has been collected. Analysis of the data using the self-rotation function shows that tetramers have approximate 222 symmetry and are positioned on a crystallographic 2-fold axis.

Clostridium↗