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Biomedical subjects

Y Inaba

Publications and source records attributed to Y Inaba.

At least 271 records · Page 15Linked to original sources
PubMed1985

A case of internal carotid artery occlusion successfully treated by encephalo-duro-arterio-synangiosis.

In many cases, intracranial occlusive strokes in children are treated symptomatically, with a diagnosis of acute infantile hemiplegia. This is because angiography is more difficult in children and, even if occlusive cerebral arterial disease is diagnosed, there is no definite therapeutic procedure. A case is reported of left internal carotid artery occlusion presenting with acute infantile hemiplegia and followed by repeated transient ischemic attacks. Encephalo-duro-arterio-synangiosis--developed as an operation for moyamoya disease--was performed on this patient, resulting in a marked revascularization of the brain in 8 postoperative months with good improvement in symptoms. This suggests that the operation may be effective in chronic cerebral ischemic diseases other than moyamoya disease.

Acute Disease↗
PubMed1985

Isolation of a new serotype of bovine rhinovirus from cattle. Brief report.

Six strains of a new serological type of bovine rhinovirus were isolated in primary calf kidney cell cultures from nasal swab specimens of cattle with acute respiratory disease in Japan. They are serologically indistinguishable from one another and show no cross reaction with bovine rhinovirus types 1 and 2. We propose that the new isolates classified as bovine rhinovirus serotype 3.

Animals↗
PubMed1985

Isolation of ovine rotavirus in cell cultures. Brief report.

Three cytopathic rotavirus strains were isolated in MA 104 cells from intestinal contents of lambs with diarrhea. Pretreatment of virus with pancreatin and incorporation of a small amount of pancreatin in maintenance medium were important for establishment of the strains in these cells. The isolates showed marked cross reactions with bovine rotavirus in immunofluorescence, but no cross reaction with bovine, human, simian, equine, porcine and lapine rotaviruses in neutralization.

Animals↗
PubMed1985

Computer-analyzed EEG before and after bypass surgery. Quantification by a computerized wave form recognition method.

Chronological changes of the EEG before and after extracranial-intracranial arterial bypass surgery were studied on 12 patients with minor completed stroke of the carotid circulation. Instead of a power spectrum used in the conventional computer analysis, a wave form recognition method, which well correlated with visual assessment of the EEG, was applied to the present computer analysis of the EEG. The EEG changed mainly in the parietal, occipital, anterior and middle temporal records of the affected cerebral hemisphere. Deterioration of the EEG continued until 1 month, and improved gradually during 3 and 6 months after the operation. However, it was difficult to conclude that the EEG improved by surgery.

Adult↗
PubMed1985

Purification, induction, and distribution of placental glutathione transferase: a new marker enzyme for preneoplastic cells in the rat chemical hepatocarcinogenesis.

A polypeptide of Mr 26,000 and pI 6.7 that was markedly increased in rat livers bearing hyperplastic nodules (HNs) induced by chemical carcinogens was identified immunochemically as the subunit of neutral glutathione (GSH) transferase (GSHTase; RX:glutathione R-transferase, EC 2.5.1.18; also called GSH S-transferase) purified from placenta (GSHTase-P) and was demonstrated immunohistochemically to be localized in preneoplastic foci and HNs. In the present study, GSHTase-P has been purified from the HN-bearing liver, and the distribution and inducibility have been examined quantitatively using anti-GSHTase-P antibody. Elevation of GSHTase-P in the HN-bearing livers was also confirmed by in vitro translation of mRNAs isolated from the HN-bearing livers. The purified GSHTase-P was homogeneous in size but had two charge isomers on two-dimensional gel electrophoresis. In normal tissues, including liver, placenta, and fetal liver, the protein content of GSHTase-P was generally low but was significantly high in kidney and pancreas. In contrast, the amount of GSHTase-P in HN-bearing livers (primary hepatomas) and transplantable Morris hepatoma 5123D were several 10-fold higher than that in normal liver but were undetectably low in transplantable Yoshida ascites hepatoma AH 130. Different from ordinary drug-metabolizing enzymes, GSHTase-P was uninducible by administration of drugs and carcinogens prior to appearance of the preneoplastic foci and HNs. In addition, species specificity of GSHTase-P was low as it was crossreactive among rat, hamster, and human.

Amino Acids↗
PubMed1985

A genetic probe for identifying bluetongue virus infections in vivo and in vitro.

We have used a DNA copy of segment 3 RNA of bluetongue virus serotype 17 (BTV-17) to detect sequence homology among the equivalent segments of five U.S.A. BTV serotypes (BTV-2, BTV-10, BTV-11, BTV-13 and BTV-17) as well as 14 other BTVs isolated from different endemic areas of the world. Both by in situ and Northern hybridization all the BTV serotypes were found to have RNA that reacted with the DNA probe. No homology was detected with epizootic haemorrhagic disease virus serotype 1, a related orbivirus. The BTV-17 DNA clone has also been used to detect viral RNA in infected sheep blood. This information has led us to develop a simple and sensitive procedure for the detection of viral genome-biotinylated clone DNA hybrids in vivo or in cultured cells following direct staining with either the avidin-fluorescein complex or the streptavidin-horseradish peroxidase complex.

Animals↗
PubMed1985

[The use of fibrin glue in neurosurgical operations].

Fibrin glue has been used in 22 neurosurgical patients and following five effects which are very much helpful in neurosurgical practice were recognized. Adhesive effect--Dural defect at cranial base was closed with lyophilized dura and fibrin glue. Hemostatic effect--Fibrin glue soaked oxycel was applied for hemostasis of the bleeding from venous sinus, dura, skull edge and cut surface of tumor. Blocking and sealing effects--In cases of craniopharyngioma, cyst wall around the inserted tube was sealed with oxycel and fibrin glue. CSF leakage from open sphenoidal sinus in aneurysmal operation was closed with fibrin glue soaked gelfoam. Covering and wall strengthening effects--Unclippable aneurysms were coated and wrapped with fibrin glue and oxycel. Packing effect--Huge dead space after removal of mucocele was packed with fibrin glue. In trans-sphenoidal operation of pituitary adenoma, fibrin glue soaked muscle pieces and oxycel were packed in the dead space and sphenoidal sinus. Many other possible availabilities in neurosurgical operations were discussed. Fibrin glue is a biological product, so it can be used with more safety and affinity to the local tissue and with less reaction as foreign body than the artificial adhesives. It takes much time for the preparation of this material for the time being. If this can be prepared instantly, usefulness of this glue will be markedly increased. We have had no hepatitis nor inflammatory complications in our limited experience.

Adolescent↗
PubMed1985

[Experimental study of brain lesions after combined coaxial exposure to high-peaked pulse wave form CO2 and Nd: YAG lasers on the brain].

UNLABELLED: The CO2 laser is useful for cutting and vaporization but not for coagulation and hemostasis. On the contrary, YAG laser is effective for coagulation and hemostasis but not for cutting. The purpose of this study is to examine the effect of the exposure of combined, coaxial CO2 and YAG laser on the animal brain to supplement the advantages and draw-backs of each other. To compare these results, each of non-combined pulse wave form CO2 and YAG lasers was employed separately. MATERIALS AND METHODS: The lasers in this study were pulse wave form CO2 and YAG lasers, employed separately or simultaneously using 130 YZ of Nihon Infrared Industries Company. Japanese white rabbits were anesthetized with pentobarbital. Fronto-parietal burr holes were made, the dura was removed and then Evans blue solution was injected intravenously. The lasers were employed to the cerebral cortex without great vessels using a micromanipulator attached to the operative microscope with a distance of 30 cm. The spot size was 700 mu for CO2 laser and 1200 mu for YAG laser. The first experiment was to see the effect of nine combinations of simultaneous coaxial CO2 of 2, 4 and 8 watts and YAG lasers of 10, 20 and 40 watts, 1 sec on the brain. In the second experiment, also combining two lasers, the exposure time of YAG laser was elongated from 1 or 2 seconds into 2 or 4 seconds and the arrangement of powers was the same as that of the first experiment. The lesions were thus made in 18 different conditions.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1985

[Topographic mapping of regional cerebral blood flow by xenon-enhanced CT--introducing a simplified method as a routine examination].

Measurement of regional cerebral blood flow (rCBF) has become a routine examinations in the field of clinical neurology and neurosurgery. Therefore, various methods for measuring rCBF have been developed. Among them, xenon-enhanced CT method has many advantages compared with others. An obtainable topographic flow map, high anatomical spatial resolution, and readily available instrument, namely CT scanner, are regarded as the advantages. On the one hand, anesthetic effects of xenon gas on the patients, taking a great expense for a single examination, and a significant dose of radiation delivered to the patients are thought to be the disadvantages. Due to the disadvantages, xenon-enhanced CT is regarded as an unpractical examination. In the paper, we introduce a method with a brief xenon inhalation, named a simplified method, with which the disadvantages can be avoided. Conventional xenon-enhanced CT requires 20 to 25 minute inhalation of xenon gas in order to achieve a saturation of xenon in the cerebral tissue for a calculation of the partition coefficients (L). In the simplified method, instead of using the calculated L, L was given as 1.0 in all the cerebral regions and xenon gas was terminated at 4 minute inhalation. Four minute inhalation of xenon gas did not make any significant anesthetic effects on the patients nor the changes of physiological parameters, such as, PaCO2 and blood pressure.(ABSTRACT TRUNCATED AT 250 WORDS)

Blood Volume Determination↗
PubMed1985

[Shared antigens between BCG and tumor cells--immunotherapy with BCG for mouse tumor].

Cytotoxic activity of anti-BCG rabbit serum (A-BCG) and the antigenic relationship between BCG and tumor cells were studies. A-BCG showed high cytotoxic activity against line 10 tumor cells of guinea pig Strain 2 and Colon 26 tumor cells of BALB/c mouse but not to cells of human bladder cancer (HT 1197, HT 1376). Cell-killing activity of A-BCG against line 10 and Colon 26 was dependent on complement participation. It was also found that Colon 26 cells were heterogenous in their susceptibility to the killing activity of A-BCG. Antigenic studies between BCG and tumor cells were undertaken using on indirect immunofluorescence method and it was found that all tumor cells used in the experiment shared common antigens with BCG. In immunotherapy with BCG for Colon 26 tumors, BCG worked effectively for suppressing tumor growth. However, enhancement of tumor growth in some of the BCG-treated mice was observed.

Animals↗
PubMed1984

The organ distribution of tin and the effect of tin on concentrations of several essential elements in rabbit.

The organ distribution and the effects of administration of tin, 5 mumol/kg/day, for 6 days on calcium, copper, iron, magnesium, phosphate, and zinc concentrations in plasma, erythrocytes, liver, kidney, and spleen of adult rabbits were studied. Kidney contained the highest concentration of tin, 15.0 +/- 1.64 micrograms/g wet weight (mean +/- S.D., n = 4). The concentrations of copper, phosphate, and magnesium increased significantly in the kidney, while magnesium concentration decreased in erythrocytes 1 day after the administration of tin for 6 consecutive days. A high correlation coefficient between magnesium and phosphate was observed in all organs tested. The correlation coefficients between P vs. Cu, P vs. Ca, and Mg vs. Ca in the liver were lowered during the period of tin administration, whereas those between P vs. Zn, Mg vs. Zn, and Cu vs. Zn in the kidney disappeared after tin administration was completed.

Animals↗
PubMed1984

Regional cerebral blood flow and stroke index after left carotid artery ligation in the conscious gerbil.

We measured the regional cerebral blood flow in both hemispheres of Mongolian gerbils subjected to permanent left carotid artery ligation, using [3H]nicotine as the tracer. At 1, 3 and 6 h post-ligation, neurological signs were recorded and a stroke index score was tallied for each animal. In conscious control gerbils, mean cerebral blood flow on the left side was 1.10 +/- 0.08 (S.E.M.) ml X g-1 X min-1 at the cerebral cortex, 0.58 +/- 0.02 at the hippocampus and 0.69 +/- 0.04 at the diencephalon. Animals with a stroke index score exceeding 10 were considered symptomatic. We noted a close relationship between regional cerebral blood flow and the stroke index score. In symptomatic animals, regional cerebral blood flow in the ischemic hemisphere at 1, 3 and 6 h post-ligation was less than 0.21 ml X g-1 X min-1 at the cortex and diencephalon, and less than 0.09 ml X g-1 X min-1 at the hippocampus. We suggest that unilaterally ligated gerbils manifesting a stroke index score greater than 10 represent a good experimental model for the study of ischemia.

Animals↗