Biomedical subjects

Y L Wei

Publications and source records attributed to Y L Wei.

21 records · Page 2Linked to original sources

PubMed1990

Isolation and characterization of temperature-sensitive mutants of Saccharomyces cerevisiae.

Temperature-sensitive (ts) mutants of Saccharomyces cerevisiae Y-196 were isolated by UV irradiation and/by EMS treatment. There were 163 ts mutants isolated from 66,957 colonies. These mutants were designated as AMY-1 to AMY-163. These ts mutants grew normally at 30 degrees C, but did not grow at 38 degrees C. In an auxanographic study, we showed that AMY-46 was an absolute ts mutant, while AMY-10, AMY-15, AMY-16, AMY-49, AMY-66, AMY-88, AMY-114, AMY-126, AMY-127, AMY-129, AMY-136, AMY-150, AMY-157, AMY-158, AMY-159, AMY-161, and AMY-162 were auxotrophic ts mutants except AMY-16 had cell size 2-42% larger than that of the wild type. Cell aggregation phenomenon was found when the ts mutants were cultivated at 30 degrees C to stationary phase then cultured at 38 degrees C for 4 hr. The budding numbers at 30 degrees C were less than that at 38 degrees C. Mutant AMY-49 had the highest budding number. There was a positive correlation between the growth rate and the average budding number. However, there was a negative correlation between the growth rate at 30 degrees C and the length of long axis of cell or the size of cell.

Mutation↗

PubMed1989

A report from the NIDDK Liver Transplantation Database.

The development and pilot phase of the NIDDK LTD project has been successfully completed by recruiting and uniformly evaluating at baseline and follow-up of 176 candidates and 101 transplant patients from 5 LTD Clinical Centers (Baylor University Medical Center, Mayo Clinic, University of California at Los Angeles, University of Minnesota, and University of Pittsburgh). The first set of analyses presented in this report describes the characteristics of the candidates, recipients and donors who were enrolled in the LTD pilot study. Other findings must be regarded as trends until more data become available. Candidates who presented with fulminant or subacute liver failure as well as those with lupoid type chronic active hepatitis had the highest selection rate for transplantation while those with malignancy and alcoholic cirrhosis had the lowest. Overall, little selection occurred on the basis of age alone. Early posttransplantation recovery characterized by prolonged ICU stay, or by death or retransplantation in the ICU, were significantly influenced by operative and recipient factors. Generally, when function of other organ systems was impaired, the event rates were higher. Abnormal neurological status represented the greatest risk. For the ICU course, patients with fulminant disease fared the worst and cirrhotics the best. Long operation (greater than 8 hrs) and blood use in excess of 3,000 ml had 2-3 times the risk for long ICU stay or adverse ICU events. Surprisingly, donor and harvesting factors also played a significant role in the early posttransplantation course. Such trends were shown for drug abuser donors and for rapid hepatectomy time. The harvesting surgeon's assessment of the donor and the graft had good predictive value of the ICU course. Finally, patients who required retransplantation within 6 weeks more often had donors with histories of longer hospitalization and fewer grafts that were deemed excellent or good at harvest.

Databases, Bibliographic↗

PubMed1989

[Synthesis of isoflavones as cardiovascular drug].

On the basis of the chemical structure of daidzein, a series of isoflavones have been synthesized. The intermediates, 7-methoxy-4'-hydroxy-isoflavone(V) and 7-hydroxy-4'-methoxyisoflavone(IX) were prepared by condensation of corresponding desoxybenzoin with dimethylformamide dimethylacetal. 7-Methoxy-2-methyl-4'-hydroxyisoflavone (VII) was also prepared from desoxybenzoin IV by reacting with acetic anhydride and anhydrous sodium acetate and followed by hydrolysis. Compounds I1-5 were obtained by Mannich reaction and compounds II1,2 III1,2 were obtained by etherification of corresponding isoflavones. All compounds were tested for resistance to hypoxia in mice and I1-3, I5, II1 and III2 were shown to have significant effect. Compound I1 and I5 were also found to increase coronary blood flow.

Animals↗