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Y Masaki

Publications and source records attributed to Y Masaki.

At least 55 records · Page 3Linked to original sources
PubMed1998

Determination of Epstein-Barr virus association with B-cell lymphomas in Japan: study of 72 cases--in situ hybridization, polymerase chain reaction, immunohistochemical studies.

The association of Epstein-Barr virus (EBV) with B-cell lymphoma was examined in 72 human immunodeficiency virus-negative Japanese patients using the polymerase chain reaction (PCR) on DNA obtained from formalin-fixed paraffin-embedded tissues and an in situ hybridization (ISH) technique. EBV-encoded RNA 1 (EBER-1) was detected in 12 of 72 cases (17%); five of 33 cases (15%) of nodal B-cell lymphomas and seven of 39 cases (18%) of extranodal B-cell lymphomas. Three cases of post-bone marrow transplantation and one case of autoimmune disease (Evans syndrome) were included among seven EBER-1 positive extranodal lymphomas. A combined study of immunohistochemistry and EBER-1 revealed that some L26 positive cells were EBER-1 positive. A DNA band was also observed in 13 of 70 examined cases (19%) (four of 33 cases of nodal B-cell lymphomas (12%) and nine of 37 cases of extranodal B-lymphomas (24%)) in the PCR study using primers to detect the Bam HI-W fragment of EBV. In the immunohistochemical study using a monoclonal antibody to the latent membrane protein 1 (LMP-1) of the EBV, one of the EBV-encoded latent gene products, LMP-1, was expressed in six of 34 cases (18%) of extranodal B-lymphomas, but none of the cases with nodal B-cell lymphomas were shown to be LMP-1 positive. Oncoprotein bcl-2 was examined by immunohistochemistry and found to be expressed in seven cases of nodal lymphomas and three cases of extranodal lymphomas, and two of these nodal cases were EBER ISH positive. In EBV serology, only two cases of nodal and one case of extranodal EBER positive B-cell lymphomas revealed a reactivation pattern. In the PCR study using primers to detect the lymphocyte-determined membrane antigen (LYDMA), the same sized monoclonal bands were observed in case 36 in the PCR products from the nose and skin, suggesting the monoclonal proliferation of the tumor. These findings suggested a low incidence of EBV association with B-cell lymphomas unless patients were in an immunologically impaired condition such as post-organ transplantation or autoimmune diseases.

Adult↗
PubMed1997

Aggressive natural killer cell lymphoproliferative disorder associated with Epstein-Barr viral RNA.

Lymphoproliferative disorder of natural killer cells is a heterogeneous disorder, and an association with Epstein-Barr virus (EBV) is suggested in some cases. A Japanese male presenting with recurrent nasopharyngeal problems developed fever, generalized lymphadenopathy, and hepatosplenomegaly. Separated cells from lymph nodes were shown to have a natural killer (NK) cell, CD2(+), CD3(-), CD16(+), CD56(+), HLA-DR(+) phenotype. A progressive abnormality of hepatic function was associated with hepatorenal failure and death. A serologic study suggested reactivated EBV infection. In situ hybridization (ISH) studies showed Epstein-Barr virus-encoded RNA (EBER)-1 in lymph nodes, with lymphocytes infiltrating the liver and tissue from ethmoid sinus surgery 3 years prior to development of obvious lymphoproliferative disease. Polymerase chain reaction performed on lymph node DNA, using oligonucleotide primers specific for the EBV lymphocyte-determined membrane antigen (LYDMA) gene, revealed a single band, suggesting monoclonal proliferation of the tumor. NK activities of the lymphocytes from the lymph node and peripheral blood were markedly decreased. These findings suggest a close relationship between EBV infection and development of NK cell lymphoproliferative disorder.

Adult↗
PubMed1997

[Ethanol injection therapy to the lung cancer].

Ethanol injection was tried in patients who underwent exploratory thoracotomy. There were 4 males and 1 female, adenocarcinoma in 3 cases, large cell carcinoma in 1 case, and metastatic lung cancer in 1 case. We grasped the lung included the tumor, and injected ethanol directly into the tumor. Maximum dose of injected ethanol was within 10 ml. There were no any complications postoperatively in all patients. Postoperative course was followed by means of chest CT. Three patients whose preoperative cancer stage was early stage gained good results in local control, two patients whose preoperative cancer stage was stage III B in one patient and metastatic cancer rapidly growing in other patient had no effects. Ethanol injection into the lung tumor was easy to perform and safety, and has direct effect in local control of the tumor in especially small sized.

Adenocarcinoma↗
PubMed1997

[Mediastinal lymph-node carcinoma with no apparent primary lesion].

To determine the clinical characteristics of cases of hilar or mediastinal lymph-node cancer in which the primary lesion was not found; reports of 36 such cases were identified. The patients in those cases comprised 29 men and 7 women, whose average age was 58.4 years. The malignancies were found in hilar lymph nodes in 15 patients, mediastinal lymph nodes in 16, and both hilar and mediastinal lymph nodes in 4; 14 were adenocarcinomas, 4 were squamous-cell carcinomas, and 4 were small-cell carcinomas. The average length of cancer-free survival after surgery was 28.9 months, and the maximum was 9 years. After the affected hilar and mediastinal lymph nodes had been resected the clinical appearance of these patients was the same as if the primary tumor had been removed. We therefore believe that many patients given a diagnosis of cancer with metastases to hilar or mediastinaly lymph nodes and in whom the primary tumor cannot be located in fact have primary cancer of the lymph nodes, and that this condition should be called "primary mediastinal lymph-node carcinoma".

Adult↗
PubMed1997

[Long-term follow-up of 43 patients with Sjögren's syndrome].

OBJECTIVE: To study the long-term outcome in patients with Sjögren's syndrome (SS). METHODS: We retrospectively studied a cohort of 43 patients with SS; 31 patients with primary SS and 12 patients with secondary SS (6 patients with rheumatoid arthritis, 3 patients with mixed connective tissue disease, 2 patients with systemic lupus erythematosus and 1 patient with systemic sclerosis). Follow-up assessments were performed for 10-20 years following the initial diagnosis. RESULTS: During follow-up, 29 patients (67%) with SS developed new extraglandular manifestations including: a small amount of monoclonal gammopathy (10 patients), interstitial pulmonary disease (8 patients), malignancy (4 patients developed cancer, 1 patient developed malignant lymphoma), Raynaud's phenomenon (4 patient), peripheral neuropathy (3 patients), renal disease (2 patients), myopathy (2 patients) and others. Statistically significant differences in the salivary gland scintigraphy existed between the progressed group and the stable group at both the initial diagnosis and ten years later. Patients with anti-Ro (SS-A) antibody had a high incidence of salivary gland enlargement, hypocomplementemia, rheumatoid factor and anti-nuclear antibody. The focus scores of the labial salivary biopsy in anti-La (SS-B) antibody positive patients were significantly higher than in patients without this antibody. No statistically significant differences were noted in symptoms and signs between study entry and follow-up. CONCLUSIONS: Although sicca symptoms involving the eyes and the mouth did not progress in most of SS patients, 67.4% of SS patients developed new extraglandular manifestations during the 10-20 years follow-up period.

Adult↗
PubMed1996

High prevalence of B-cell monoclonality in labial gland biopsies of Japanese Sjögren's syndrome patients.

Patients with Sjögren's syndrome (SS) have an increased risk of developing malignant lymphoma. Although some clinical parameters may herald the imminent onset of lymphoma, few reliable markers are available to predict the progression to a malignant lymphoproliferative disorder. Although there are a number of immunological and serologic features that distinguish SS in Japanese patients, in common with their Western counterparts these patients also have an increased risk of lymphoid neoplasia. Recently we have reported finding a high prevalence (17%) of monoclonal immunoglobulin (Ig) heavy chain gene rearrangements in labial salivary gland (LSG) biopsies of Western SS using the polymerase chain reaction (PCR). In many cases this finding was predictive for the subsequent development of lymphoma. In this study LSG from 50 Japanese SS patients were examined for Ig heavy chain gene monoclonality using PCR to amplify the VDJ region and identified in 7 of 50 (14%) cases. Three patients with monoclonality in the LSG had evidence of lymphoma at extra-salivary gland sites. In one of these the diagnosis of lymphoma was made subsequent to lip biopsy. In the other two lymphoma at extra-salivary gland sites was diagnosed prior to LSG biopsy. The results suggest that the prevalence of Ig heavy chain gene monoclonality in LSG of Japanese SS patients is similar to that in the West, and that neoplastic cells can be identified in LSG as a component of more widely disseminated disease.

Aged↗
PubMed1996

[Effect of sevoflurane on release of TNF-alpha and IL-1 beta from human monocytes].

Although isoflurane inhibits TNF-alpha and IL-1 beta release from human monocytes stimulated by LPS in dose dependent fashion, it is unclear whether sevoflurane has the same effects. Therefore, we investigated whether sevoflurane could inhibit TNF-alpha and IL-1 beta secretions from human monocytes stimulated by LPS in dose dependent fashion in vitro. Human monocytes stimulated by LPS were cultured for 3 h in the presence of sevoflurane 1% or 5%. Another group of human monocytes were cultured in the absence of sevoflurane. TNF-alpha and IL-1 beta increased after stimulation of LPS and these increases were not inhibited by sevoflurane in a dose dependent fashion. We conclude that sevoflurane does not inhibit TNF-alpha and IL-1 beta release from monocytes stimulated by LPS.

Anesthetics, Inhalation↗
PubMed1996

[Adoptive immunotherapy for pancreatic cancer, using MUC1 specific CTL].

A new therapeutic strategy for pancreatic cancer is needed because of its very poor prognosis. We presented the results of adoptive immunotherapy and new adoptive immunotherapy using MUC1 specific CTL. We expect that these new strategies may help improve the prognosis of pancreatic cancer.

Cancer Vaccines↗