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Y Mito

Publications and source records attributed to Y Mito.

At least 19 recordsLinked to original sources
PubMed1997

Dentatorubral pallidoluysian atrophy (DRPLA) protein is cleaved by caspase-3 during apoptosis.

Dentatorubral pallidoluysian atrophy (DRPLA) is an autosomal dominant neurodegenerative disorder. It is associated with an abnormal CAG repeat expansion resulting in formation of a protein with an elongated polyglutamine stretch. However, neither the physiological roles of the DRPLA gene product nor molecular mechanisms of its pathogenesis have yet been elucidated. Here we report that the DRPLA protein is cleaved at a site near the N terminus during apoptosis induced by VP-16, staurosporine, or glucocorticoid. Moreover, the in vitro translated DRPLA protein is cleaved by recombinant caspase-3, a member of the cysteine protease family, which is thought to be a main executioner of apoptosis. Using mutant DRPLA proteins, the cleavage site was identified as 106DSLDG110. The cleavage, however, was not modulated by the length of the polyglutamine stretch. These findings suggest that the DRPLA protein is one of the physiological substrates of caspase-3, and its cleavage may result in structural and biochemical alterations associated with apoptosis.

Amino Acid Sequence↗
PubMed1997

Neurological manifestations of primary Sjögren's syndrome in Japanese patients.

The neurological manifestations of twenty-one Japanese patients with Sjögren's syndrome (SjS) were evaluated. All patients were women, and sixteen of the twenty-one cases (76%) demonstrated objective abnormal neurological symptoms, the most frequently observed of which was trigeminal neuropathy (50%). Multiple mononeuropathy was seen in almost one-third of the examined cases (31%). Central nervous system (CNS) involvement was observed in three cases (14%). All of these values differed greatly from those previously reported. Therefore, this study revealed characteristic features of Japanese SjS and also implied the existence of different immunopathological mechanisms associated with SjS in Japanese patients.

Adult↗
PubMed1997

Infiltrating cell profiles of sarcoid lesions in the muscles and peripheral nerves: an immunohistological study.

In order to study the pathological mechanisms of neuromuscular sarcoidosis, we carried out an immunohistochemical investigation in five cases (five muscle specimens and two sural nerve specimens). We evaluated the distribution of inflammatory mononuclear cells and major histocompatibility complex (MHC) antigen expressions. Our data showed a dominant infiltration of helper/inducer T cells (CD4-T cells), suggesting the importance of cell-mediated immune responses in neuromuscular sarcoidosis. However, we could not identify the distinct distributional patterns of T cells as reported in sarcoid lymphadenitis. This result may be attributed to the difference of the affected organs. Moreover, failure to detect class II antigens in the muscle fibers may imply the difference in pathogenic mechanism between neuromuscular sarcoidosis and other inflammatory myopathies.

Aged↗
PubMed1995

[Development of new in vitro chemosensitivity test using collagen gel droplet embedded culture and its clinical usefulness].

We developed a new in vitro assay for chemosensitivity test using collagen gel droplet embedded culture and image analysis. In this in vitro assay, we successfully minimized the cancer cell number required for culture to approximately 3-10 x 10(3) cells for each 30 microliters collagen gel droplet, obtained the sufficient growth of cancer cells using serum-free medium while suppressing the growth of fibroblastic cells, and measured the volume of cancer cells by eliminating the contaminating fibroblastic cells by an image processing technique. Anticancer effects of the in vitro assay showed a very good correlation with those of in vivo nude mouse assay using human cancer cell lines. The success rates of the in vitro assay for 141 surgical specimens of primary lung cancers and for 65 of primary breast cancers were 89 and 80%, respectively. The accumulated in vitro assay response rates of MMC, CDDP, VDS and VP-16 for primary lung cancers and of MMC, 5-FU and ADR for primary breast cancers were similar to the respective clinical response rates. These results suggest that this in vitro chemosensitivity test may be practically useful for clinical applications.

Animals↗
PubMed1995

[Leukotriene].

Explore the source record for details and available documents.

Humans↗
PubMed1994

MR appearance of subacute combined degeneration of the spinal cord.

Subacute combined degeneration (SCD) of the spinal cord is well known to produce degenerative lesions in the spinal cord histopathologically, but a few reports on the neuroradiological findings have so far been reported. We presented the MRI findings of the spinal cord in a case of SCD. The localization of the radiologically proven lesions was similar to that of the previous pathological reports.

Aged↗
PubMed1994

Neuromyelitis optica.

An 11-year-old girl developed sudden visual loss in her left eye, preceding subacute myelitis by 9 months. Multifocal lesions in the central nervous system were demonstrated by magnetic resonance imaging. Although unilateral blindness is unusual, the clinical findings predominantly involving the optic nerve and spinal cord were consistent with the diagnosis of neuromyelitis optica (NMO). This case provides additional evidence to support the view that NMO is a variant of multiple sclerosis.

Blindness↗
PubMed1993

[Pharmacokinetics of flomoxef in children undergoing chronic hemodialysis].

The pharmacokinetics of flomoxef (FMOX), an oxacephem antibiotic, were investigated in pediatric patients undergoing chronic hemodialysis. The results are summarized as follows. 1. FMOX was given intravenously in a single dose of 10 mg/kg to 5 pediatric patients (ranging from 7 to 15 years of age) undergoing chronic hemodialysis. It was also given in a dose of 5 mg/kg to 2 of these patients. Its concentrations in serum and urine were determined using bioassay. Pharmacokinetic analyses were performed using a two compartment open model. 2. The serum concentrations of FMOX, administered in doses of 10 mg/kg or 5 mg/kg on non-hemodialysis days, were 33.3 +/- 4.09 micrograms/ml (mean +/- standard deviation) and 17.6 micrograms/ml (mean) at 30 minutes after injection, 29.6 +/- 3.51 micrograms/ml and 15.9 micrograms/ml at 1 hour, 27.2 +/- 2.14 micrograms/ml and 15.1 micrograms/ml at 2 hours, 23.5 +/- 1.90 micrograms/ml and 13.0 micrograms/ml at 4 hours, 20.8 +/- 2.44 micrograms/ml and 12.2 micrograms/ml at 6 hours, 18.9 +/- 1.86 micrograms/ml and 11.0 micrograms/ml at 8 hours, and 9.64 +/- 1.43 micrograms/ml and 6.16 micrograms/ml at 24 hours, respectively. 3. The urinary concentrations of FMOX, administered in a dose of 10 mg/kg, were 42.4-123 micrograms/ml between 0-6 hours after injection, 14.1-52.5 micrograms/ml between 6-24 hours, and 2.86-23.7 micrograms/ml between 24-48 hours. The urinary excretion rate in the first 48 hours after injection ranged from 9.1 to 10.5%.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1989

[Mass-screening for acid mucopolysaccharidosis using urine samples by modified HCl-albumin turbidity method].

To establish a method for screening acid mucopolysaccharidosis (AMPS), the HCl-albumin turbidity method was reexamined and modified for semiquantitative analysis. Mucopolysaccharide samples and HCl-albumin in buffer solution were incubated at 37 degrees C for 30 minutes, and the optical density at 600 nm (OD 600) of the reaction mixtures were measured. When the same amount of the samples were tested, OD 600 of dermatan sulfate and hyaluronic acid were higher than that of keratan sulfate. When the cut-off point of OD 600 was set at 0.100 based on the values obtained from randomly selected subjects, only 3 of 328 urine samples (0.9%) showed value higher than the cut off value. The values in five patients with AMPS were all above 0.800 without overlapping those of the control subjects. These results suggest that mass-screening for AMPS using urine sample is possible by the partially modified HCl-albumin turbidity method with the cut-off point of OD 600 set at 0.100.

Adolescent↗
PubMed1980

Conversion of positive to negative chemotaxis by Ca2+ ionophore treatment in plasmodium of Physarum polycephalum.

The plasmodium of Physarum polycephalum was treated with Ca2+ ionophores A23187 or X-537A, and the effects on the chemotactic and contractile activities were studied by applying a double-chamber method and an optical method. (1) At low Ca2+ concentration, attractants (glucose, L-alanine) KH2PO4) remained as attractants and induced a relaxation in the plasmodial strand. Increase in the external Ca2+ level caused a contraction of plasmodium with the same stimuli, and hence converted the attractants to repellents. (2) This phenomenon was specific to Ca2+; Mg2+, Sr2+ or Ba2+ showed no appreciable effects. (3) Repellents (sucrose, KCl, Quinine) induced a contraction, which was not altered by the Ca2+ ionophore treatment. (4) Efflux of Ca2+, which was measured by chemiluminescence of aequorin, increased on glucose reception in the presence of ionophore.

Anti-Bacterial Agents↗
PubMed1980

Baroreflex sensitivity in renal failure.

1. Baroreflex sensitivity was evaluated in 22 non-dialysed patients with chronic renal failure secondary to chronic glomerulonephritis. Baroreflex sensitivity was judged by the slope of the linear regression of the pulse interval on the rise in systolic blood pressure with injection of phenylephrine or reduction by amyl nitrite inhalation. 2. Baroreflex sensitivity was reduced in these patients as compared with normal controls. Reduction of baroreflex sensitivity was significantly greater in nine hypertensive than 13 normotensive patients with chronic renal failure. 3. A significant positive correlation was found between baroreflex sensitivity and motor nerve conduction velocity measured on ulnar nerve in 13 patients examined. 4. Saline was given with high dietary salt intake to seven normotensive patients with chronic renal failure for 2 or 5 days in order to determine whether the severe depression of baroreflex sensitivity can be an initiating factor for hypertension. Blood pressure was raised to hypertensive levels within 5 days in two patients in whom baroreflex sensitivity was nearly as low as that of hypertensive patients, but not in five cases whose baroreflex sensitivity was normal or only mildly depressed. Plasma volume increased to the same degree in both groups. Baroreflex sensitivity did not change in the former two cases despite blood pressure elevation. 5. It is concluded that reduced baroreflex sensitivity in chronic renal failure correlated with the prescence ofhypertension, as well as uraemic neuropathy, and may be one of the pathogenetic mechanisms of hypertension in end-stage chronic glomerulonephritis.

Adult↗
PubMed1980

Selective suppression of positive chemotaxis in Physarum polycephalum by treatment with rotenone or under anaerobic condition.

The chemotactic motive force of plasmodia of Physarum polycephalum was measured by the double-chamber method. The treatment of plasmodia with 0.1 mM rotenone did not affect the motility of the palsmodia but led to suppression of the chemotaxis toward all the attractants examined (glucose, galactose, c-AMP, KH2PO4). Rotenone treatment did not affect the chemotaxis against repellents (fructose, NaCl). Similar results were obtained when the chemotactic motive force was measured under an anaerobic condition (95% N2-5% CO2).

Anaerobiosis↗