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Y Uchimura

Publications and source records attributed to Y Uchimura.

At least 37 records · Page 2Linked to original sources
PubMed1995

[Application of AmpliType PM kit to forensic sciences].

Five genetic loci, low density lipoprotein (LDLR), glycophorin A (GYPA), hemoglobin G gammaglobin (HBGG), D7S8 and group specific component (GC), can be identified in a single step by the AmpliType PM kit. The kit uses multiplex PCR amplification and hybridization by a reverse dot blot method. To introduce the new marker system in forensic caseworks in Japan, a relevant Japanese population database must be established. In this context, the allele frequencies of the 5 genetic loci were determined for 246 unrelated Japanese. The allele frequencies were LDLR*A 0.173, LDLR*B 0.827, GYPA*A 0.528, GYPA*B 0.472, HBGG*A 0.352, HBGG*B 0.648, HBGG*C 0.000, D7S8*A 0.612, D7S8*B 0.388, GC*A 0.258, GC*B 0.504 and GC*C 0.238, respectively. In all 5 systems, the genotype frequencies are in good accordance with the Hardy-Weinberg expectations. The power of discrimination (PD) of LDLR, GYPA, HBGG, D7S8 and GC were 0.449, 0.624, 0.600, 0.612 and 0.788, respectively, resulting in a combined PD of 0.993, while these 5 DNA types could be determined from slaiva and semen. And the GYPA or GC genotypes were observed to have good relations with the MN and serum Gc phenotypes. The AmpliType PM kit was confirmed to be a reliable DNA typing system which was well suited for use in forensic sciences.

Alleles↗
PubMed1993

Purification of blood-group substances in human hepatic bile (HHB) and immunological characterization of anti-HHB serum.

Blood-group substances (BGS) in human hepatic bile (HHB) of blood type A were purified 3657-7314-fold by ethanol sedimentation and column chromatography with DEAE Sephadex A-50 and Concanavalin A Sepharose 4B. An anti-HHB serum was raised by immunizing rabbits with the purified BGS in the HHB. Cross-reactions of the native anti-HHB serum with such human materials as type A blood cell, plasma, seminal fluid and saliva were eliminated by stepwise sequential absorptions with type A red blood cells, type O seminal fluid and type AB plasma. Precipitin titers of the native anti-HHB serum were x 320 and x 80 and those of the absorbed anti-HHB serum x 80 and x 20 against the immunogen and HHB, respectively. Organ and species specificity of the absorbed anti-HHB serum was checked by counter immunoelectrophoresis. It reacted specifically only with the immunogen and HHBs of various ABO blood types. No reactions with animal gallbladder biles were observed. In practical examinations, the specific anti-HHB serum identified 15 out of 20 (75%) HHB samples and 41 out of 46 (89%) human faeces samples. No reactions with animal gallbladder biles and animal faeces were observed.

ABO Blood-Group System↗
PubMed1991

Nonradioactive labeling with chemically modified cytosine tails by the polymerase chain reaction.

We developed a modified nonradioactive method for the detection of DNA. This method makes use of the polymerase chain reaction for preparation of probes; that is, a DNA fragment inserted in the polylinker region of an M13 or pUC vector is amplified with primers that have a modified cytosine tail at the 5' terminus (C-tailed primers). By this method, large amounts of labeled probes can be obtained easily. After hybridization, modified cytosine tails can be detected immunologically. DNA labeled by this method could be used in plaque hybridization. We could detect 0.05 pg of dot-blotted labeled DNA in 30 min with an enzyme-catalyzed chemiluminescence reaction.

Base Sequence↗
PubMed1991

[A case report of advanced gastric cancer responsive to radiotherapy and CDDP arterial injection].

A 48-year-old-male patient with advanced gastric cancer, which extended from corpus to antrum of the stomach and metastasized to the left lobe of the liver, was treated with radiotherapy combined with CDDP arterial injection. Total irradiation and CDDP dosage was 50 Gy/25 f and 150 mg (75 mg/mal/body), respectively. The regression of the tumor was remarkable. For inoperable advanced gastric cancer, radiotherapy combined with anticancer drugs proved a worthwhile therapy, and it was necessary to use the pharmacological characteristics of the drugs.

Adenocarcinoma↗
PubMed1990

[Case report of the superior approach for correction of the supracardiac type of total anomalous pulmonary venous drainage (TAPVD) in the neonatal period].

An eleven-day-old newborn with cyanosis and respiratory distress was admitted. Intubation and catecholamine administration had already done. Blood pressure was 80/50 mmHg, heart rate was 160/min. Urine volume was only 7 ml per 4 hours with diuretics. The diagnosis of supracardiac type of TAPVD was done by echo cardiography. Without cardiac catheterization, emergent operation was performed. After median sternotomy, the mobilization of the aorta was done. Under cardiopulmonary bypass the aorta was retracted to the left, the superior vena cava to the right and the common pulmonary venous trunk (CPV) was identified through the transverse sinus. Parallel incision were made in the left atrium and CPV, and extended to the vertical vein. An anastomosis using continuous suture was fashioned. She had a good postoperative course. The superior approach through the transverse sinus affords excellent exposure of the CPV and left atrium in situ in the neonatal period, if the mobilization of the aorta is completed, and the heart is not compressed or displaced. This approach is useful for supracardiac type of TAPVD.

Female↗
PubMed1989

The primary structure of porcine liver acylamino acid-releasing enzyme deduced from cDNA sequences.

Two overlapping cloned cDNAs encoding the entire amino acid sequences of the subunits of acylamino acid-releasing enzyme (AARE) [EC 3.4.19.1] have been isolated from porcine liver cDNA lambda gt10 cDNA libraries and sequenced. Sequence analyses of the cDNA and several Achromobacter protease I-digested peptides of the purified protein revealed that porcine liver AARE consists of four identical subunits, and each comprising a single chain of 732 amino acids with acetylmethionine at the N-terminus.

Animals↗
PubMed1982

Unusual personal identification with characteristic features in chest roentgenograms.

This case illustrates the unexpected aspects of radiographic personal identification. A skeletonized body without the neck and head, upper extremities, and feet was discovered on a seashore. No positive results were obtained from the remains concerning either the cause of death, the manner of death, or precise identification of the body. Roentgenologic examinations of the remains revealed a marked eminence of the manubrium sterni with characteristic crossing shadows. A successful partial superimposition of the ante- and postmortem lateral chest x-ray films helped to place the personal identification as that of a fisherman, 58 years old, missing in the sea for 21 days.

Forensic Medicine↗
PubMed1980

Puffer poisoning in Japan--a case report.

A fatal case of puffer poisoning in an 80-year-old man is reported. By using a bioassay with mice and extracts of crude poisons, the toxicities of the autopsy materials, fresh puffer ovaries, and the remaining cooked ovaries were determined and expressed in mouse units per gram (M.U.). Some medicolegal aspects of puffer poisoning are also discussed. Puffer poisoning is a primary cause of fatal food poisoning in Japan even though its frequency is not high.

Adult↗
PubMedDate not supplied

The influence of putrefactive changes on the determination of Paraquat in autopsy materials.

This paper describes how the putrefactive changes in a cadaver will influence the determination of Paraquat in autopsy material. The carcasses of rats that had been given large doses of Paraquat and subsequently killed by cervical dislocation, were left at room temperature for up to seven days. Samples of stomach, caecum and liver were removed for the colorimetric determination of Paraquat with sodium dithionite following extraction on a Dowex 50W-4 column. Paraquat recovered from the stomach and caecum showed a decrease with time after death with the rate of decrease being greater in the caecum samples. However, the concentration of Paraquat in the liver increased for three days and then began to decrease gradually. The possible causes of these variations in Paraquat recovery are discussed. The findings appear to demonstrate the value of Paraquat determination even in samples from material that is in a state of advanced putrefaction.

Animals↗