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Biomedical subjects

Yan Wang

Publications and source records attributed to Yan Wang.

At least 163 records · Page 9Linked to original sources
PubMed2006

[Change of oxygen free radical in patients with obstructive sleep apnea hypopnea syndrome after the operation of uvulopalatopharyngoplasty].

OBJECTIVE: To explore the mechanism of multiple organ dysfunction in patients with obstructive sleep apnea hypopnea syndrome (OSAHS) by observing the change of SOD,GSH-PX and MDA after uvulopalatopharyngoplasty (UPPP). METHOD: Colorimetric method was used to examine both the activity of SOD,GSH-PX and the content of MDA before and one month after UPPP in 30 patients with OSAHS. Thirty healthy people were chosen as normal control. RESULT: Before UPPP, the activity of SOD, GSH-PX and the content of MDA were (106.3 +/- 41.2) nmol/L (compared with the control group, P < 0.05), (61.8 +/- 6.5) kU/L (P < 0.05) and (6.27 +/- 1.73) mmol/L (P < 0.05) respectively; yet after the operation, the results were(128.5 +/- 33.1) nmol/L (compared with the control group, P > 0.05), (64.6 +/- 6.1) kU/L (P > 0.05) and (1.32 +/- 0.58) mmol/L (P > 0.05). CONCLUSION: The metabolism of oxygen free radical was badly imbalanced in patients with OSAHS, yet one month after UPPP, it was recovered to normal. This suggested that the metabolism imbalance of oxygen free radical was contributed to multiple organ dysfunction in such patients.

Adult↗
PubMed2006

[SSR polymorphism on the waxy gene locus and their relationship to amylose content in wheat].

Variation of one simple sequence repeat (SSR)(AT (AT)n AT) in the 3 end of the waxy gene was analyzed in samples of 32 cultivars, including different wheat cultivars in Shandong region and representing a wide distribution range of amylose content (AC) in cultivated wheat. In this study, we found two fragments were present in all cultivars, one of 204bp, the expected size, and one of 225-346bp. 204bp fragment located on the chromosome 7DS, while 225-346bp fragment located on the chromosome 7AS. That is, Wx-Ala gene and Wx-Dla gene present in all wheat lines, but the larger fragment showed length polymorphism. The result showed that the amylose level is highly positive correlated with the length of the polymorphic SSR. Amplified fragment is long in high AC cultivars and electrophoresis migration rate is small, while the fragment is short in low AC cultivars and electrophoresis migration rate is large. AC is highly diverse among different lines with the various SSR genotypes. Although the SSR variation did not seem to have obvious function in the synthesis of the starch synthase encoded by the waxy gene, the correlation between the SSR and AC level could be used as molecular marker for quality improvement in wheat programs.

Amylose↗
PubMed2006

[Electroantennogram and behavioral responses of Monochamus alternatus to the volatiles from Pinus thunbergii with different physiological status].

The volatiles from healthy and pinewood nematode-infested branches of Pinus thunbergii were collected by distillation, and analyzed by using HPLC and GC-MS. Electroantennogram (EAG) and behavioral responses of Monochamus alternatus with different physiological status to the volatiles were investigated respectively. The results showed that unmated beetle had a greater EAG response potential to the volatiles from healthy branch than to those from infested branch, while it was reverse for mated beetle. The EAG response of unmated beetle under 15 days-old to the volatiles from healthy branch increased with its age. "Y" tube olfactory test showed unmated beetle had positive response to the volatiles from healthy branch and negative response to those from infested branch, while mated beetle showed positive response to the volatiles from infested branch and negative response to those from healthy branch. Female beetle with its age from 1 day to 15 days-old and male beetle from 1 day to 9 days-old had an increasing positive response to the volatiles from healthy branch, but the male after 9 days-old showed a negative response. It could be concluded that M. alternatus with different physiological status all had special sensitivity and selectivity to host tree.

Animals↗
PubMed2006

[Radiation from mobile phone and the health].

With the development of the mobile communication industry, there are considerable public concerns about the relationship between mobile phone radiations and human health. With regard to electromagnetic field emitted by mobile phone maybe influence tumor,learning and memory, subjective symptoms and blood-brain barrier, a large number of studies have been performed abroad. This paper reviews the research development abroad on these problems, and analysizes the mechanism, through which the mobile phone effects on human health.

Animals↗
PubMed2006

[Identification of two novel mutations of human blood coagulation factor V gene in a Chinese family with congenital factor V deficiency].

OBJECTIVE: To discover the mutations of human blood coagulation factor V (FV) gene in a Chinese family with congenital factor V deficiency, and to explore the molecular mechanism associated with the congenital factor V deficiency. METHODS: PCR and DNA sequencing were used to look for the FV gene mutations in the proband. And the novel mutation were testified by PCR restriction fragment length polymorphism technique or reverse DNA sequencing. One hundred healthy volunteers were chosen as controls at random. RESULTS: Two novel mutations were discovered in the FV gene of proband, which were the A1763C missense mutation in exon 11 and the splicing site mutation in the 3' terminal of intron 16 (G-->T). The pedigree analysis showed that the two mutations inherited from his parents respectively: the A1763C came from his father, and the G-->T from his mother. The A1763C missense mutation in exon 11 was not found in each of 100 healthy volunteers. CONCLUSION: The congenital deficiency of FV in the proband might be caused by the A1763C missense mutation in exon 11 and the splicing site mutation in the 3' terminal of intron 16, which jointly caused the proband to be a double heterozygote.

Base Sequence↗
PubMed2006

[Arousal response in child patients in obstructive sleep apnea].

OBJECTIVE: To analyze arousal response of Polysomnography (PSG) in children with obstructive sleep apnea-hypopnea syndrome (OSAHS). METHOD: Fourty-eight children with sleep disorder were studied with 32 guide Polysomnography and analyzed by Polysmith. Sleep structure and arousals of preschool-aged and school-aged children with OSAHS were compared with those of primary snore patients. RESULT: The mean value of AHI of preschool aged children with OSAHS was (10.6+/-8.9) h, of AI was (4.0+/-7.1) h, of HI was (6.6+/-4.6) h; The sleep structure between OSAHS group and primary snore group was not significant (P > 0.05). The mean value of ArI with AHI was (8.2+/-5.9) h, of ArI with LM was (19.6+/-5.8) h, whole ArI was 49.3+/-20.4 in preschool-aged children with OSAHS; The mean value of ArI with AHI was (7.6+/-6.3) h, of ArI with LM was (14.3+/-7.6) h, whole ArI was 49.9+/-14.9 in school-aged children with OSAHS. Those of OSAHS children were higher than that of primary snore patients (P < 0.05). CONCLUSION: Although OSAHS in children is characterized by a restless sleep, the structure is perfect and mildly affected by OSAHS. The ArI with AHI, with LM and the whole ArI in OSAHS children is higher than those of primary snore patients.

Arousal↗
PubMed2006

[Study on determination of entrapment efficiency of sinomenine liposomes].

OBJECTIVE: To establish an HPLC method for the determination of entrapment efficiency of sinomenine liposomes. METHOD: The liposomes and dissociated drugs were separated by sephadex filtration, mini-column centrifugation and dialysis. The methodology study and the optimization of determining condition were carried out at the same time. RESULT: Sephadex filtration could effectively separate the sinomenine liposomes from dissociated sinomenine. The column recovery was 98.8%, the average entrapment efficiency of three tests was64.9%, RSD 2.67%. CONCLUSION: The method was simple, exact, and had a good reappearance. It can be used to examine the entrapment efficiency of sinomenine liposomes.

Dextrans↗
PubMed2006

Association between the polymorphism in the promoter region of dopamine D4 receptor gene and chronic tic disorder.

OBJECTIVE: To study a possible association between the three functional polymorphisms in the promoter region of dopamine D4 receptor (DRD4) gene and chronic tic disorder. METHODS: Genomic DNA was isolated from the venous blood leukocytes of 84 unrelated patients with chronic tic disorder (Study group) and 100 healthy unrelated individuals (Control group). Polymorphisms of DRD4, 1240L/S, 616C/G and 521C/T, were genotyped by the allele-specific primer (ASP) PCR. Genotype, allele and haplotype frequencies were analysed by SHEsis online. RESULTS: There were significant differences in both allele and genotype frequencies (chi(2) = 8.419, P < 0.01; chi(2) = 7.860, P < 0.05 respectively) of DRD4-616C/G between the Study and the Control groups. Haplotypic frequencies of LCT (1240L/S, 616C/G, 521C/T) in the Study group were noticeably higher than in the Control group (chi(2) = 6.371, P < 0.05). CONCLUSIONS: There is an association between the DRD4-616C/G polymorphism and chronic tic disorder. The individuals with haplotype LCT (1240L/S, 616C/G, 521C/T) are susceptible to this disorder.

Adolescent↗
PubMed2006

[The expression and clinical significance of survivin gene in leukemia].

OBJECTIVE: To investigate the expression of survivin in leukemia and the prognostic significance in acute leukemia (AL). METHODS: The expression of survivin mRNA was measured in 105 AL and 21 chronic myelogenous leukemia (CML) patients with semi-quantity reverse transcription (RT)-PCR. 15 adults were tested as normal control (NC) and K562, NB4, Kg-1alpha, HL-60 cell lines were also tested as positive control. The cell cycle distribution in AL was measured with flow cytometry (FCM) to analyze the relationship between the level of survivin and cell proliferation. RESULTS: The expression of survivin in de novo AL (0.525 +/- 0.460) was higher than that in NC (0.101 +/- 0.187), while it decreased in complete remission (CR) patients (0.280 +/- 0.095). In replased patients (0.935 +/- 0.343), the expression of survivin increased again. There was no difference of the expression between chronic phase of CML (0.279 +/- 0.112) and NC, but in acute phase of CML (0.653 +/- 0.236), the expression was higher than that in NC. The cases with higher level of survivin had significantly higher proliferation indices (mean PI 9.682) than those (mean PI 6.899). In AL patients, the CR rate of survivin positive cases was lower than that of survivin negative cases. CONCLUSIONS: The PI in survivin positive patients was significantly higher than that in survivin negative indicating its impact on proliferation and cleavage. Abnormal expression of survivin genes was related to pathogenesis and progression of AL and it can serve as a marker of relapse and poor prognosis in AL. Overexpression of survivin is a risk factor for CML.

Case-Control Studies↗
PubMed2006

[Angiotensin II up-regulates expression of inducible nitric oxide synthase in human umbilical endothelial cells: roles of AT1 and AT2].

OBJECTIVE: Angiotensin II is an important pro inflammation factor in the cardiovascular system. This experiment is aimed to study the effects of angiotensin II on inducible nitric oxide synthase expression in human umbilical endothelial cells. METHODS: Human umbilical endothelial cells were cultured in vitro and treated with angiotensin II alone or in combination with AT1, AT2 and NF-kappaB inhibitors respectively. The inducible nitric oxide synthase expressions at protein and mRNA levels were measured with Western blot and reverse transcription-polymerase chain reaction (RT-PCR), and the activity of NF-kappaB was analyzed with EMSA. RESULTS: Angiotensin II up-regulated inducible nitric oxide synthase expressions at the protein and mRNA levels at 5 h (P < 0.05), the activity of NF-kappaB was enhanced at 2 h (P < 0.05). These effects could be blocked by AT1 and NF-kappaB inhibitors but not by AT2 inhibitor. CONCLUSION: Angiotensin II can upregulate the expression of inducible nitric oxide synthase through NF-kappaB pathway in human umbilical endothelial cells. AT1, other than AT2, play a key role in this process.

Angiotensin II↗
PubMed2006

[Effect of cardiotrophin-1 on cardiac transcription factor GATA4 expression in rat cardiomyocytes].

OBJECTIVE: To investigate the effect of cardiotrophin-1 (CT-1) on the GATA4 expression and related signaling pathways (JAK-STAT3, ERK1/2 and PI3-K) in rat cardiomyocytes. METHODS: Using semi-quantitative RT-PCR and EMSA, we measured the dose and time dependent effects of CT-1 on GATA4 mRNA and binding activity in cultured rat cardiomyocytes. Parthenolide (a STAT inhibitor), U-0126 (an ERK inhibitor) and LY-294002 (a PI3-K inhibitor) alone or in combination were added to the culture medium to assess the role of above signaling pathways in CT-1 mediated effects. RESULTS: GATA4 mRNA expression significantly increased at 3 h post 0.1 nmol/L CT-1 exposure, peaked at 6 h and remained high till 24 h post exposure. The GATA4 binding activity began to increase at 10 min and peaked at 60 min and returned to baseline level 180 min. Six hours post CT-1 (0.01 nmol/L, 0.1 nmol/L, 1 nmol/L) exposure, the GATA4 mRNA expression increased in a dose-dependent manner. The GATA4 binding activity peaked with 0.1 nmol/L CT-1 and higher dose did not further increase the binding activity. U-0126 increased the GATA4 mRNA expression and enhanced the GATA4 binding activity and these effects could be partially attenuated with addition of Parthenolide. Parthenolide also prevented the increase of GATA4 mRNA and binding activity induced by CT-1. LY-294002 had no effects GATA4 mRNA and binding activity. CONCLUSION: CT-1 increases the GATA4 mRNA expression and binding activity in rat cardiomyocytes via STAT3/ERK1/2 pathways and these effects are independent of PI3-K pathway.

Animals↗
PubMed2006

[The effects of amniotic membrane on corneal penetration of ofloxacin].

OBJECTIVE: To evaluate the corneal penetration of ofloxacin following amniotic membrane transplantation (AMT). METHODS: One hundred and eight New Zealand rabbits were divided into 6 groups: mechanical remove of corneal epithelium with and without AMT; corneal alkali burn with and without AMT, and normal cornea with and without AMT. Topical ofloxacin was administered four times at interval of every 15 minutes. Five min, 30 min, 1 h, 2 h, 4 h and 6 h after the last administration, 3 rabbits from each group were sacrificed and aqueous humor was collected. The concentration of ofloxacin was detected by high pressure liquid chromatography (HPLC). RESULTS: The ability of ofloxacin to penetrating cornea was improved obviously in de-epithelialized groups and cornea alkali burn groups no matter AMT was performed or not (P < 0.05). The concentration of ofloxacin in the aqueous humor in normal cornea with and without AMT showed no statistical difference within 1 h after administration. But at the last three time points, the ofloxacin concentration in AMT group was greater than that in the group without AMT (P < 0.05). The comparison between the de-epithelialized with and without AMT obtained the similar result. At every tested time point, the ofloxacin concentration in aqueous humor of corneal alkali burn with AMT group was statistically greater than that of the group without AMT (P < 0.05). CONCLUSION: Amniotic membrane could store and release the drug slowly, results in the increase of contact time between ofloxacin and corneal surface and promotes the corneal penetration of ofloxacin.

Amnion↗
PubMed2006

Operative strategy of acetabular fractures.

Anatomic structure of acetabular fractures are complex and operative exposure and fixation are extremely difficult. For those obviously displaced acetabular fractures, close reduction is doomed to cause deformed healing. Open reduction with internal fixation (ORIF) not only results in anatomic reduction, but also brings complications. No matter which method will be adopted, traumatic arthritis or avascular necrosis of femoral head might occur. In order to treat acetabular fractures more effectively, orthopedic surgeons should be required to fully master the acetabular anatomy, biomechanics, classification and the necessary knowledge for complication prevention.

Acetabulum↗
PubMed2006

[Application of wavelength selection algorithm to measure the effective component of Chinese medicine based on near-infrared spectroscopy].

Near infrared (NIR) spectroscopy has raised a lot of interest in the pharmaceutical industry because it is a rapid and cost-effective analytical type of spectroscopy with no need for extensive sample preparation, and with the easy-realizable ability of on-line application. The NIR technology can increase the quality control standard of the Chinese medicine and accelerate the entry into the international market. In the present paper, two methods for wavelength selection are applied to the measurement of borneol, one of which is the multiple-chain stepwise, which tends to select many variables in the same area containing valuable information, and the other is the mixture genetic algorithm, which incorporates simulated annealing so as to improve the local searching ability while maintaining the global searching ability. The results present that the number of wavelength is reduced to 16% compared with the original number of wavelength, and the prediction accuracy has increased 47.6%. Therefore, the method of wavelength selection is a good way to enhance the prediction accuracy and simplify the model in NIR region.

Algorithms↗
PubMed2006

[Human excited electroencephalogram model and its location in brain].

The aim of this study was to identify the characteristics of human excited electroencephalogram and the excited location in brain. The subjects were excited by taking a fixed quantity of caffeine. Electroencephalographic signals were collected using with 128 channels Phoenix Digital EEG and compared before and after the subjects drank coffee. The results showed obvious differences and compared with the ones before being excited electroencephalogram. After being excited we found that the lower frequency composition was restrained in the region below 10 Hz, the high frequency composition was increased significantly,and a wave crest of 38 Hz was produced in every acquisition point with the height around 200. Then the excited region was located in brain with the software of ASA 3 Course (designed by ANT company of Germany) and found that the excited location was focused on the area of the middle abdomen in the pons' side near bulbar when a person was exciting.

Adult↗
PubMed2006

[Survey of application of acupuncture and moxibustion in Seychelles].

Survey of application of acupuncture and moxibustion in Seychelles are introduced from acupuncture and moxibustion methods, accepting degree of patients and therapeutic effects, etc.. The authors hold that acupuncture and moxibustion have superiority in treatment of pain diseases, especially bony arthritis of loin and knee in Seychelles, and indicate that application of acupuncture and moxibustion are influenced by recognition for acupuncture and moxibustion, medical system, problem in acupuncture and moxibustion, quality of acupuncturist, and other factors.

Acupuncture Therapy↗
PubMed2006

[Discussion of some problems in the new centurial planning material the therapeutics of acupuncture and moxibustion].

Some problems were found for argument in the teaching of The Therapeutics of Acupuncture & Moxibustion, the new centurial national advanced Chinese medical school's planning material, including errors in compiling, some concepts lacking definition, and some acupuncture treatment methods in the material deviating off clinical practice, etc.. Study on these problems so as to make the teaching material more perfect, more scientific, accurate and authoritative.

Acupuncture↗