Biomedical subjects

Yining Shi

Publications and source records attributed to Yining Shi.

3 recordsLinked to original sources

PubMed2006

DNA sequencing and multiplex STR analysis on plastic microfluidic devices.

The ability of plastic microfluidic devices with separation channel lengths of 6, 10 or 18 cm to perform high-quality and high-performance ssDNA analysis was evaluated. Specifically, four-color DNA sequencing separation of a terminator sequencing standard using replaceable, urea-denaturing linear polyacrylamide (LPA) solution as a sieving matrix, yielded read lengths of 410 bases in 15 min with base calling accuracy of 99.2% on a 6-cm device, and 640 bases in 35 min with accuracy of 98.0% on a 18-cm device. A two-color sizing analysis of four-locus (CSF1PO, TPOX, TH01, vWA) short tandem repeats (STRs) allelic ladder on a 10-cm device indicated a mean SD of +/- 0.08 base pairs (bp) between runs, and single bp resolution of spiked TH01 allele 9.3 (198 bp) from TH01 allele 10 (199 bp) of the CTTv ladder with R = 0.81. A four-color multiplex sizing analysis of three different AmpFlSTR allelic ladders consisting of nine loci (D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820) and gender alleles (Amelogenin) on a 10-cm device had a mean SD of +/- 0.15 bp between runs for sizing three loci, i.e., FGA, D18S51 and D3S818; alleles differing by 2 bp in size were resolved with resolutions close to baseline. This work demonstrates that plastic microfluidic devices are capable of quality sequencing and STR sizing comparable to that of glass devices of similar separation lengths.

DNA, Single-Stranded↗

PubMed2005

[The cross-sectional study of dynamic refractive status in Xi'an middle school students].

PURPOSE: To obtain the basic data of refractive status, especially the myopia prevalence rate in Xi'an middle school students. METHODS: The cross-sectional study was conducted in two Xi'an city middle schools. The object ophthlmic examinations such as non-cycloplegic objective refraction, visual acuity, oculr pressure and fundus evaluation were taken on all students by special trained ophthalmologists and nurses. The special designed inventory scale was filled in and input in a special computer program. Then the data were statistically analyzed with SPSS11.0. RESULTS: (1) In the 3711 middle school students (average age of 13-18 years), the prevalence rate of myopia was 91.2%, including 51.7% low myopia, 31.8% moderate myopia, 7.7% high myopia, 5.7% emmetropia and 3.1% hypermetropia. Among 6 grades, the constituents of moderate and high myopia groups were significantly increased with the age growing. (2) The average diopter of spherical equivalent refraction was (-2.76 +/- 2.24)D, that of low myopia was (-1.68 +/- 0.76)D, moderate myopia (-4.35 +/- 0.84)D and high myopia (-7.47 +/- 1.33)D. In the junior 1st grade the average diopter of spherical equivalent refraction was (-1.57 +/- 1.66)D, the junior 2nd, (-1.94 +/- 2.04)D,the junior 3rd, (-3.26 +/- 2.09)D, the senior 1st grade' (-3.29 +/- 2.49)D, the senior 2nd, (-3.09 +/- 2.31)D and the senior 3nd, (-3.57 +/- 2.25) D. There were significant differences (P=0.000) in the average diopter of spherical equivalent refraction in all the 6 grades. However, there was no significant difference among the junior 3rd, senior 1st and senior 2nd grade. (3) The degree of myopia in female students [(-2.88 +/- 2.25)D] and the right eyes [(-2.86 +/- 2.19)]D was significantly higher than that in male students [(-2.65 +/- 2.23)D] (P = 0.000) and the left eyes [(-2.67 +/- 2.29)D] (P = 0.009) respectively. CONCLUSION: The constituesnts of myopia were definitely changed in and above the junior 3rd grade; meanwhile the progression of myopia in female students and the right eyes is earlier than that in male students and the left eyes'. The result indicates that interventions of myopia progression performed at the stage may retard the myopia progression and reduce the prevalence of myopia.

Adolescent↗

PubMed2003

High-resolution single-stranded DNA analysis on 4.5 cm plastic electrophoretic microchannels.

Plastic microchannels (4.5 cm long) fabricated from an etched glass master were tested for high-resolution single-stranded DNA analysis. Using replaceable denaturing linear polyacrylamide as sieving matrix, one-color separation of a fragment sizing standard with single-base resolution (R > 0.5) was achieved up to 275 bases. Two-color sizing analysis of four loci short tandem repeat (STR) allelic ladder (CSF1PO, TPOX, TH01, vWA) with single-base resolution (R = 0.62) on TH01 alleles 9.3 (198 bp) and 10 (199 bp) was demonstrated. An average standard deviation of +/- 0.06 bp and +/-0.11 bp in sizing 32 alleles of the CTTv ladder was attained between runs and between channels, respectively. Four-color sequencing separation of a terminator sequencing standard showed a base-calling accuracy of 99.1% out to 320 bases in 13 min.

Acrylic Resins↗