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PubMed · 14595684

High-resolution single-stranded DNA analysis on 4.5 cm plastic electrophoretic microchannels.

Yining Shi·Rolfe C Anderson
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Abstract

Plastic microchannels (4.5 cm long) fabricated from an etched glass master were tested for high-resolution single-stranded DNA analysis. Using replaceable denaturing linear polyacrylamide as sieving matrix, one-color separation of a fragment sizing standard with single-base resolution (R > 0.5) was achieved up to 275 bases. Two-color sizing analysis of four loci short tandem repeat (STR) allelic ladder (CSF1PO, TPOX, TH01, vWA) with single-base resolution (R = 0.62) on TH01 alleles 9.3 (198 bp) and 10 (199 bp) was demonstrated. An average standard deviation of +/- 0.06 bp and +/-0.11 bp in sizing 32 alleles of the CTTv ladder was attained between runs and between channels, respectively. Four-color sequencing separation of a terminator sequencing standard showed a base-calling accuracy of 99.1% out to 320 bases in 13 min.

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Acrylic ResinsAllelesColorDNA, Single-StrandedElectrophoresis, CapillaryFluorescent DyesMicrofluidicsSequence Analysis, DNATandem Repeat SequencesPolyacrylamidesJournal Article

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BibTeXRIS

Yining Shi, Rolfe C Anderson. 2003. High-resolution single-stranded DNA analysis on 4.5 cm plastic electrophoretic microchannels.. https://doi.org/10.1002/elps.200305553

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