Biomedical subjects

Yuguang Zhao

Publications and source records attributed to Yuguang Zhao.

5 recordsLinked to original sources

PubMed2006

Effect of dielectric properties of metals on terahertz transmission subwavelength hole arrays.

We study the influence of dielectric function of metals on the transmission properties of terahertz pulses through periodically patterned subwavelength holes. Because of a drastic increase in the value of dielectric constants, most metals become highly conductive at terahertz frequencies. Extraordinary terahertz transmission is observed in subwavelength hole arrays made from both good and poor electrical conductors. The measured transmittance of terahertz pulses is found to be enhanced with increasing ratio of the real to the imaginary dielectric constant of the constituent metals, for which the dielectric function follows the Drude model.

Journal Article↗

PubMed2006

Terahertz demonstrations of effectively two-dimensional photonic bandgap structures.

We demonstrate effectively two-dimensional (2D) terahertz (THz) photonic bandgap (PBG) structures for transverse electromagnetic (TEM) mode propagation within metal parallel plate waveguides (PPWG). The 2D-PBG structures consisting of square arrays of dielectric cylinders were characterized by THz time-domain spectroscopy (THz-TDS). THz photonic bandgaps were observed, as determined by the 160 mum lattice constant, the 65 mum diameter, and the dielectric constant of the cylinders. The experimental measurements were fitted with excellent agreement to 2D theory, confirming that for TEM mode propagation, effectively 2D propagation experiments can be achieved within the bounded space of the PPWG.

Journal Article↗

PubMed2006

Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek's disease.

Herpesvirus of turkey (HVT) is an alphaherpesvirus that is widely used as a live vaccine against Marek's disease because of its antigenic relationship with Marek's disease virus (MDV). In spite of a similar genome structure, HVT has several unique genes, the functions of which are not completely understood. As a first step in carrying out detailed analysis of the functions of the HVT genes, a full-length infectious bacterial artificial chromosome (BAC) clone of HVT was constructed. DNA from two independent BAC clones, upon transfection into chicken embryo fibroblasts, produced plaques similar to those produced by the wild-type virus. Viruses derived from the BAC clones were stable during in vitro passage, but showed differences in in vitro growth kinetics compared with the wild-type virus. Using a one-step mutagenesis protocol to delete the essential glycoprotein B gene from the HVT genome, followed by construction of the revertant virus, BAC clones of HVT were shown to be amenable to standard mutagenesis techniques. In spite of the difference in in vitro growth, viruses from both clones induced 100 % protection against infection by the virulent MDV strain RB-1B, indicating that the BAC-derived viruses could be used as vaccines with efficacies similar to that of the parental virus. The construction of HVT BAC is a major step in understanding the functions of HVT genes by exploiting the power of BAC technology. Furthermore, the availability of the BAC clones enables use of HVT as a vector for expressing foreign genes.

Animals↗

PubMed2004

Determination and comparison of specific activity of the HIF-prolyl hydroxylases.

Hypoxia-inducible factor (HIF) is a transcriptional complex that is regulated by oxygen sensitive hydroxylation of its alpha subunits by the prolyl hydroxylases PHD1, 2 and 3. To better understand the role of these enzymes in directing cellular responses to hypoxia, we derived an assay to determine their specific activity in both native cell extracts and recombinant sources of enzyme. We show that all three are capable of high rates of catalysis, in the order PHD2=PHD3>PHD1, using substrate peptides derived from the C-terminal degradation domain of HIF-alpha subunits, and that each demonstrates similar and remarkable sensitivity to oxygen, commensurate with a common role in signaling hypoxia.

Amino Acid Sequence↗

PubMed2003

Improving baculovirus recombination.

Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an essential viral gene that lies adjacent to the locus used for recombination. Viral DNA prepared from the knockout fails to initiate an infection unless rescued by recombination with a baculovirus transfer vector. Modified viral DNA allows 100% recombinant virus formation, obviates the need for further virus purification and offers an efficient means of mass parallel recombinant formation.

Animals↗