Improving baculovirus recombination.

Yuguang Zhao; David A G Chapman; Ian M Jones

doi:10.1093/nar/gng006

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Improving baculovirus recombination.

Yuguang Zhao·David A G Chapman·Ian M Jones

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Abstract

Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an essential viral gene that lies adjacent to the locus used for recombination. Viral DNA prepared from the knockout fails to initiate an infection unless rescued by recombination with a baculovirus transfer vector. Modified viral DNA allows 100% recombinant virus formation, obviates the need for further virus purification and offers an efficient means of mass parallel recombinant formation.

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Animals Bacterial Proteins Baculoviridae Cell Line DNA, Viral DNA-Binding Proteins Escherichia coli Escherichia coli Proteins Exodeoxyribonucleases Gene Expression Genes, Viral Green Fluorescent Proteins Luminescent Proteins Mutation Recombinant Fusion Proteins Recombination, Genetic Transfection Journal Article Research Support, Non-U.S. Gov't

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Yuguang Zhao, David A G Chapman, Ian M Jones. 2003-01-15. Improving baculovirus recombination.. https://doi.org/10.1093/nar%2Fgng006

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