Biomedical subjects

Yun-qin Chen

Publications and source records attributed to Yun-qin Chen.

3 recordsLinked to original sources

PubMed2004

[Analysis of pregnancy outcomes after in vitro fertilization-embryo transfer and intracytoplasmic sperm injection].

OBJECTIVE: To compare pregnancy and perinatal outcomes between in vitro fertilization-embryo transfer (IVF-ET) and intracytoplasmic sperm injection (ICSI). METHODS: A retrospective study was carried out to measure pre-clinical and clinical abortion, ectopic pregnancies, multiple gestations, birth weight, gestational age, congenital malformation and perinatal mortality in patients receiving either IVF-ET (n = 143, group 1) or ICSI (n = 173, group 2) from January 1999 to June 2001. The outcomes of singleton and twin were compared separately. RESULTS: The maternal age, infertility duration, parity and the number of transferred embryo were comparable between the two groups. There were no significant differences in abortion rate (16.1% vs 13.3%), birth rate (65.7% vs 74.6%) between IVF-ET and ICSI groups (P > 0.05). In singleton, the rates of low birth weight, small for gestational age and pre-term birth were 1.8%, 7.3%, 5.5% respectively in IVF-ET group and 6.8%, 8.1%, 14.9% respectively in ICSI group. In twin, the rates of low birth weight, small for gestational age and pre-term birth were 34.2%, 30.3%, 42.1% respectively in IVF-ET group and 42.6%, 38.0%, 46.3% respectively in ICSI group. There were no significant differences between the two groups (P > 0.05). But the rates of low birth weight, small for gestational age and pre-term birth were higher in twin than in singleton (P < 0.01). The incidence of congenital malformation was 2.2% and 1.6% in IVF-ET and ICSI group respectively (P > 0.05). CONCLUSIONS: The pregnancy and perinatal outcomes are similar between IVF-ET and ICSI groups. Twin is the main cause of low birth weight, small for gestational age and pre-term birth.

Adult↗

PubMed2003

[A new method for detecting serum beta-lipoprotein electrophoretogram].

OBJECTIVE: To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE). METHODS: After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE. RESULTS: s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified. CONCLUSION: s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.

Electrophoresis, Polyacrylamide Gel↗

PubMed2003

Features of serum beta-lipoprotein electrophoretogram in patients with coronary heart disease.

OBJECTIVE: To investigate the changes in serum beta-lipoprotein (beta Lp) electrophoretogram in the patients with coronary heart disease (CHD). METHODS: This study included 36 CHD patients, 28 non-CHD patients and 8 normal control subjects, from whom serum beta Lps (prestained with Sudan Black B) were isolated by means of the first double-gradient polyacrylamide gel electrophoresis (f-DGPAGE). The secondary DGPAGE was performed for isolating beta Lps into 4 subfractions (beta Lp1-4) in order to complete the quantitative analysis of beta Lp electrophoretogram. A parallel test to examine the serum concentration of low-density lipoprotein cholesterol (LDLc) was also carried out. RESULTS: The relative amount of beta Lp1 was significantly elevated in CHD patients (53.4+/-10.0%) as compared with normal subjects (39.3+/-6.7%) and non-CHD patients (42.8+/-6.8%, P < 0.05 for both comparisons) regardless of serum LDLc concentrations, and beta Lp1 content between the latter 2 groups showed no significant difference. CONCLUSION: Serum beta Lp subfraction imbalance evidenced by increased beta Lp1 level characterizes the patients with CHD.

Adult↗