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PubMed · 12581967

[A new method for detecting serum beta-lipoprotein electrophoretogram].

Yun-qin Chen·Jia Yin·Min Zhang·Bing-sheng Yin
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Abstract

OBJECTIVE: To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE). METHODS: After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE. RESULTS: s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified. CONCLUSION: s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.

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Electrophoresis, Polyacrylamide GelHumansIsoelectric FocusingLipoproteins, LDLReproducibility of ResultsEnglish AbstractJournal Article

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Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin. 2003. [A new method for detecting serum beta-lipoprotein electrophoretogram].. https://pubmed.ncbi.nlm.nih.gov/12581967/

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