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Z Walter

Publications and source records attributed to Z Walter.

39 records · Page 3Linked to original sources
PubMedDate not supplied

Isolation of the adducts of platinum complexes and nucleic acid bases on the Dowex 50 W column.

The method presented here enables the isolation of three monofunctional adducts. These are: platination of cytosine, adenine and guanosine. The three detected bifunctional complexes were: guanine N7 to N7, adenine N7 to N7 and adenine N7 to N1. A mixed bifunctional complex of guanine and adenosine and the product of polymerization of three adenines and two moieties of cis DDP were also detected. In the peak eluted separately from the guanine standard, no Pt was detected. The sample eluted in this peak had UV spectrum different from the standard and may represent the degraded product of guanine and polymerized Pt.

Adenine↗
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Isolation of the products resulting from the reaction of cis and trans diaminedichloroplatinum [II] with DNA and chromatin on the Dowex 50 W column.

The production of platinated derivatives of nucleic acid bases resulting from the reaction of cis and trans DDP with DNA and chromatin was studied. Bifunctional complex of guanine appeared to be the major product of the interaction of cis isomer with both DNA and chromatin, although other bifunctional adducts of A-Pt-G and A-Pt-A were also isolated. The main product of the interaction of trans DDP with DNA was a monofunctional adduct of guanine. Small amounts of the bifunctional complexes were also isolated. When ssDNA was incubated with trans DDP more bifunctional complexes appeared, what suggests that geometric constrains of double helix prevent formation of these complexes. Trans isomer reacts more easily with chromosomal proteins than cis DDP does. Therefore after the reaction of trans DDP with chromatin less platination occurs on DNA moieties.

Anion Exchange Resins↗