Biomedical subjects

Zhao-hui Li

Publications and source records attributed to Zhao-hui Li.

5 recordsLinked to original sources

PubMed2005

[Investigation of TiO2 photocatalytic degradation of bovine serum albumin].

The present paper reports photocatalytic degradation of bovine serum albumin (BSA) by TiO2 under near UV (365 nm) irradiation. Ultraviolet-Visible spectrometry, FTIR spectrometry and HPLC were used to study the degradation and characterize the final degradation products. It was found that under near UV irradiation, BSA (0.1 g x L(-1)) can be totally degraded to small fragments by TiQ2 in 5 h. Total mineralization of BSA (93.5%) is reached after reacting for 40 h. C, N and S in BSA are mineralized to give CO2, NO3- and SO4(2-). It is suggested that the degradation of BSA happens in two steps: first to small fragments, then further mineralized to small inorganic molecule. The absence of UV irradiation or TiQ2 can only lead to partial degradation of BSA (30%) in 5 h and no CO2 is observed even in longer reaction time.

Animals↗

PubMed2004

[Study on the changes of electrocardiogram and ultrastructural in heroin dependence in rats].

OBJECTIVE: To study the changes of electrocardiograms (ECG) and myocardial ultrastructure in heroin dependence in rats, in order to reveal the mechanisms of the myocardial injury by heroin. METHODS: Establish heroin addict model in SD mice, investigate the changes in electrocardiograms, HE staining and myocardial ultrastructure. RESULTS: The electrocardiograms of the addict group had prominently changes, main expressions: heart rate decreased, P wave and T wave amplitude reduced and duration increased, S-T reduced and duration increased, QT interval prolongation, these changes indicated that myocardium had been injured, myocardial ischemia, ventricle function declined. These difference was significant (P<0.05) between before inject heroin and after inject heroin. Transformations in the ultrastructure: nuclear concentrate, reduce, nuclear membrane shrink, chromatin agglutinate, mitochondria cristal had disorder formation, disappeared or hollowed, these indicated that heroin could cause pathological changes in myocardial ultrastructure. CONCLUSION: Above-mentioned changes indicated that heroin can injure myocardium, and the changes of myocardial ultrastructure suggested that myocardial apoptosis may be one of the mechanisms of the myocardial injury by heroin.

Animals↗

PubMed2004

[The damage of cardiovascular system in heroin abuses].

The heroin abuses can seriously damage human body system, among them the damage of cardiovascular system is various. In this paper those damages involved heart rate, blood pressure, electrocardiogram, heart function, blood circulation, the changes of some material inside, and complications of cardiovascular system are reviewed.

Arrhythmias, Cardiac↗

PubMed2003

[New improved approaches for DYS385 detection].

OBJECTIVE: To establish more sensitive methods for detection of DYS385 in routine forensic casework. METHODS: The primers recommended by Genome Database (GDB) and Schneider were used to amplify DYS385 respectively. Then, a semi-nested PCR of DYS385 was designed by using the two different primers as outer and inner primer. A series of experiments were carried out to achieve good result by adjusting the ratio of outer/inner primer and optimizing the PCR condition. RESULTS: It showed that an overall 112 bp shorter DYS385 fragments and better electrophoretic separation were obtained by using primer2B. By using the semi-nested PCR approach, the shorter specific DYS385 fragments could be amplified and detectable DNA amounted to 50 pg. CONCLUSION: This method is 20 fold more sensitive than the ordinary method.

Chromosomes, Human, Y↗

PubMed2003

[Expression of the glycoprotein in atrioventricular cell membrane of acute myocardial infarction in human].

OBJECTIVE: To study the expression of the glycoprotein in atrioventricular cell membrane of the acute myocardial infarction. METHODS: The glycoprotein changes occurred at the atrioventricular cell membrane of the acute myocardial infarction of 8 cases were observed by using immunohistochemical methods. RESULTS: Positive staining of PNA could be observed in atrioventricular cell membrane. CONCLUSION: This experiment proved that atrioventricular cell membrane expressed D-galactose as same as myocardial cell membrane in the acute myocardial infarction.

Atrioventricular Node↗