[New improved approaches for DYS385 detection].

Shuang-bo Tang; Jing-yuan Guo; Zhao-hui Li

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PubMed · 12725164

[New improved approaches for DYS385 detection].

Shuang-bo Tang·Jing-yuan Guo·Zhao-hui Li

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Abstract

OBJECTIVE: To establish more sensitive methods for detection of DYS385 in routine forensic casework. METHODS: The primers recommended by Genome Database (GDB) and Schneider were used to amplify DYS385 respectively. Then, a semi-nested PCR of DYS385 was designed by using the two different primers as outer and inner primer. A series of experiments were carried out to achieve good result by adjusting the ratio of outer/inner primer and optimizing the PCR condition. RESULTS: It showed that an overall 112 bp shorter DYS385 fragments and better electrophoretic separation were obtained by using primer2B. By using the semi-nested PCR approach, the shorter specific DYS385 fragments could be amplified and detectable DNA amounted to 50 pg. CONCLUSION: This method is 20 fold more sensitive than the ordinary method.

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Chromosomes, Human, Y Forensic Medicine Gene Frequency Humans Male Polymerase Chain Reaction Sequence Analysis, DNA Tandem Repeat Sequences English Abstract Journal Article

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Shuang-bo Tang, Jing-yuan Guo, Zhao-hui Li. 2003. [New improved approaches for DYS385 detection].. https://pubmed.ncbi.nlm.nih.gov/12725164/

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