Analysis of thrombotic factors in severe acute respiratory syndrome (SARS) patients.
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Adult↗
Biomedical subjects
Zhong Hua Liu
Publications and source records attributed to Zhong Hua Liu.
[In vivo transduction of EGFP into female mouse reproductive system by electroporation and microbubble-enhanced sonoporation].
In the present study, the efficiency of in vivo transduction of EGFP plasmid DNA into adult female mouse reproductive system by means of electroporation and microbubble-enhanced sonoporation are discussed. In the first experiment,EGFP plasmid DNA was injected into the ovary and following electroporation (square electric pulses were applied five times at 60V with a constant time of 20ms). Green fluorescence was detected 10h after transduction. In the second experiment, plasmid DNA was mixed with the microbubble at different ratio and injected into the uterus and the ovary separately. The tissues were exposed to different combined ultrasound parameters immediately after injection. We found that the suitable parameter was 2 w/cm2 intensity, 20% duty cycle and 5min. Under this condition, green fluorescence was expressed in the uterus endometrium and ovarian cortex 12 h post-sonoporation and the expression lasted up to 5 days.
Animals↗
[Interspecies nuclear transfer using nonquiescent somatic cell].
It is the point at issue in intraspecies nuclear transfer whether quiescence is necessary for development of nuclear transfer reconstructed embryos. In the interspecies nuclear transfer, some reports have proved that quiescent cell is able to support preimplantation development of the interspecies reconstructed embryos. Are non-quiescent cells able to support preimplantation development of the interspecies reconstructed embryos? We used non-quiescent somatic cells from C57BL/6 mice and giant pandas as donors to transfer into enucleated rabbit oocytes. After electrofusion (the electrofusion rates were 62.2% and 71.6%, respectively) and electrical activation, 5.1% of those mouse-rabbit reconstructed embryos developed to blastocyst in vitro, and 4.2% of panda-rabbit reconstructed embryos developed to blastocyst after transferring into ligated rabbit oviduct. These results indicate that non-quiescent cell from C57BL/6 mouse and giant panda could be dedifferentiated in enucleated rabbit oocytes and support early embryo development.
Animals↗
[Effects of different treatments on oocytes activation and parthenogenesis in mouse].
In order to study effects of electro-fusion and strontium chloride (SrCl2) activation in nuclear transfer experiment on activation and development of mouse oocytes, concentration and treatment duration of SrCl2, electro-pulse and electro-pulse combining SrCl2 were used to activate mouse oocytes which were obtained after hCG 17h. Activated oocytes were cultured in vitro in CZB medium. The results were as follows: 82.4% activation percentage was obtained when the oocytes were treated with 10mmol/L SrCl2 for 6h, it was significantly (P>0.05) higher than those obtained from that treated with the 5mmol/L or 10mmol/L SrCl2 for 4h. The activation percentage was not significantly different between 5mmol/L and 10mmol/L SrCl2 for 6h, but the percentage of morula and blastocyst in 10mmol/L SrCl2 6h group was significantly (P > 0.05) higher than those in 5mmol/L SrCl2 6h group. In the groups of treatment with electro-pulse, the best activation percentage (70.9%) was obtained when the oocytes were treated with 1.0kv/cm, 320micros, 3 pulses, but M + B percentage (7.9%) was low. In the groups of treatment with electro-pulse combining with SrCl2, the best result was acquired (activation and M + B percentage were 75.0% and 57.3% separately) when the oocytes were treated in 10mmol/L SrCl2 for 6h interval 1h after treated with 1.8kv/cm, 10s, 1pulse. These results show that the treatment with electro-pulse combining SrCl2 is a better way to mouse parthenogenesis.
Animals↗