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PubMed · 15344320

[Interspecies nuclear transfer using nonquiescent somatic cell].

Jin Song Li·Zhi Ming Han·Zhi Yu Zhu·Duan Cheng Wen·Zhong Hua Liu·Min Kang Wang·Li Lian·Jun Du·Peng Yan Wang·He Min Zhang·Da Yuan Chen
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Abstract

It is the point at issue in intraspecies nuclear transfer whether quiescence is necessary for development of nuclear transfer reconstructed embryos. In the interspecies nuclear transfer, some reports have proved that quiescent cell is able to support preimplantation development of the interspecies reconstructed embryos. Are non-quiescent cells able to support preimplantation development of the interspecies reconstructed embryos? We used non-quiescent somatic cells from C57BL/6 mice and giant pandas as donors to transfer into enucleated rabbit oocytes. After electrofusion (the electrofusion rates were 62.2% and 71.6%, respectively) and electrical activation, 5.1% of those mouse-rabbit reconstructed embryos developed to blastocyst in vitro, and 4.2% of panda-rabbit reconstructed embryos developed to blastocyst after transferring into ligated rabbit oviduct. These results indicate that non-quiescent cell from C57BL/6 mouse and giant panda could be dedifferentiated in enucleated rabbit oocytes and support early embryo development.

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AnimalsBlastocystCells, CulturedEmbryo TransferFemaleMiceMice, Inbred C57BLNuclear Transfer TechniquesOocytesRabbitsUrsidaeEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Jin Song Li, Zhi Ming Han, Zhi Yu Zhu, Duan Cheng Wen, Zhong Hua Liu, Min Kang Wang, Li Lian, Jun Du, Peng Yan Wang, He Min Zhang, Da Yuan Chen. 2002. [Interspecies nuclear transfer using nonquiescent somatic cell].. https://pubmed.ncbi.nlm.nih.gov/15344320/

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