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PubMed · 10029680

Stabilization of the intermediate in frameshift mutation.

D Sagher·A Hsu·B Strauss
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Abstract

A mismatch repair, proofreading deficient mutant of Escherichia coli lost a C from a C8 run at a rate 10 times higher than the loss of A from an A8 sequence in the same double mutant. This greater frameshift instability of a homopolymeric run of C's may be due to stabilization of a stacked intermediate. Gain of a (CA) unit in a similarly constructed (CA)15 sequence occurred at a rate about 1/3 that previously reported for a (CA)14 construct losing a (CA) repeat unit.

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Base SequenceDNA, BacterialEscherichia coliFrameshift MutationMolecular Sequence DataMutagenesis, InsertionalRepetitive Sequences, Nucleic AcidTemplates, GeneticJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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D Sagher, A Hsu, B Strauss. 1999-01-25. Stabilization of the intermediate in frameshift mutation.. https://doi.org/10.1016/s0027-5107(98)00227-9

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