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PubMed · 10331483

Dramatic decrease of circulating levels of monocyte chemoattractant protein-1 in Kawasaki disease after gamma globulin treatment.

M Terai·T Jibiki·A Harada·Y Terashima·K Yasukawa·S Tateno·H Hamada·S Oana·H Niimi·K Matsushima
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Abstract

Kawasaki disease (KD) is a systemic vasculitis preferentially affecting coronary arteries. Extensive monocytes/macrophages infiltrate in the vascular lesions, implying the involvement of a chemotactic cytokine in their recruitment. We investigated the role of monocyte chemoattractant protein-1 (MCP-1, also termed monocyte chemotactic and activating factor) in KD. In the immunohistochemical studies using the cardiac tissues of patients with fatal KD, MCP-1 but not interleukin (IL) -8 or macrophage inflammatory protein-1alpha was localized at the extracellular matrix associated with mononuclear cellular infiltration. The sites of MCP-1 expression correlated with the distribution of the acute inflammation, including early coronary vasculitis. In prospectively studied patients with KD, circulating levels of MCP-1, IL-8, tumor necrosis factor alpha (TNF-alpha), and IL-1alpha were elevated in 73, 77, 57, and 0% of samples before gamma globulin (GG) treatment (400 mg/kg x 5 days = total 2 g/kg), respectively, compared with respective control values. GG treatment correlated with a rapid decrease in the circulating levels of MCP-1 (P = 0.001) but not IL-8 (P = 0.19) or TNF-alpha (P = 0.33). In the sensitive Western blotting, MCP-1 bound to GG. Furthermore, GG inhibited the MCP-1-induced Ca2+ influx in a human monocytic cell line in vitro. These findings suggest a role of MCP-1 in KD, and indicate that GG treatment may block MCP-1 activity, thus alleviating KD vasculitis.

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Blotting, WesternCalciumCell LineChemokine CCL2Chemokine CCL4ChildChild, PreschoolHumansImmunohistochemistryInfantInterleukin-1Interleukin-8Macrophage Inflammatory ProteinsMonocytesMucocutaneous Lymph Node SyndromeMyocardiumTumor Necrosis Factor-alphagamma-GlobulinsClinical TrialJournal ArticleResearch Support, Non-U.S. Gov't

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M Terai, T Jibiki, A Harada, Y Terashima, K Yasukawa, S Tateno, H Hamada, S Oana, H Niimi, K Matsushima. 1999. Dramatic decrease of circulating levels of monocyte chemoattractant protein-1 in Kawasaki disease after gamma globulin treatment.. https://doi.org/10.1002/jlb.65.5.566

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