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PubMed · 10349658

Screening for protein-protein interactions.

F J Germino·N K Moskowitz
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Abstract

The assays described above can be used to screen for cellular proteins that can interact with a protein of interest, to screen for mutant proteins that retain the ability to bind to its partner, and to identify the domains and amino acids involved in known protein-protein interactions. Nonetheless, the biological significance of some of these interactions needs to be confirmed by appropriate cellular studies.

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Alcohol DehydrogenaseAmino Acid SequenceAnimalsBase SequenceChromosome MappingCloning, MolecularConserved SequenceCyclin-Dependent Kinase Inhibitor p21CyclinsDNA, ComplementaryDNA-Binding ProteinsEscherichia coliFishesFungal ProteinsGene LibraryGenes, ReporterMolecular Sequence DataPlasmidsPromoter Regions, GeneticRecombinant Fusion ProteinsRestriction MappingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsTranscription, Geneticbeta-GalactosidaseJournal Article

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F J Germino, N K Moskowitz. 1999. Screening for protein-protein interactions.. https://doi.org/10.1016/s0076-6879(99)03026-8

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