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PubMed · 10349659

Using the Lac repressor system to identify interacting proteins.

N L Stricker·P Schatz·M Li
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Abstract

The present protocol describes a series of procedures to identify peptides interacting with PDZ domains. It is conceivable that the procedures can be applied to other purified protein modules or intact proteins without substantial modifications. With the deduced consensus combined with sequence information, it is possible to identify proteins present in the database with compatible sequences. If the expression of target protein and potential interacting candidate overlap temporally and spatially, biochemical and molecular experiments can be designed to study their physical and functional interactions.

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AnimalsBacterial ProteinsBinding SitesCloning, MolecularConsensus SequenceDNA, ComplementaryDatabases as TopicEnzyme-Linked Immunosorbent AssayEscherichia coliEscherichia coli ProteinsGene LibraryGlutathione TransferaseLac RepressorsNitric Oxide SynthaseNitric Oxide Synthase Type IPeptide LibraryRecombinant Fusion ProteinsRepressor Proteinssrc Homology DomainsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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N L Stricker, P Schatz, M Li. 1999. Using the Lac repressor system to identify interacting proteins.. https://doi.org/10.1016/s0076-6879(99)03027-x

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