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Failure of gamma-interferon to decrease mortality from group B streptococcal sepsis in neonatal rats.

R R Wittler·R W Harris·D D Paine
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Abstract

Newborns possess an altered immune response to infection with impaired leukocyte chemotaxis and deficient production of gamma-interferon (IFN-gamma). IFN-gamma enhances neonatal leukocyte activation and movement. We proposed that IFN-gamma in conjunction with penicillin compared to penicillin therapy without IFN-gamma would increase survival from group B streptococcal sepsis in a neonatal rat model. Newborn rats were infected with 10(5) cfu of group B streptococci at 48-72 h of age and randomized to receive either serum albumin (controls), rat recombinant IFN-gamma, albumin and penicillin, or IFN-gamma and penicillin. Survival 120 h postinfection revealed: controls 5% (1/21); IFN-gamma 4% (1/24); penicillin 23% (5/22); and IFN-gamma plus penicillin 10% (2/21). Survival analysis with a lognormal parametric regression model revealed only the penicillin group to have improved survival compared to controls. Contrasting the penicillin group with the IFN-gamma plus penicillin group did not reveal a statistically significant difference by the Wald chi2 statistic (p = 0.25).

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AnimalsAnimals, NewbornBacteremiaInterferon-gammaPenicillinsRatsRats, Sprague-DawleyRecombinant ProteinsStreptococcal InfectionsStreptococcus agalactiaeSurvival RateJournal Article

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R R Wittler, R W Harris, D D Paine. 1999. Failure of gamma-interferon to decrease mortality from group B streptococcal sepsis in neonatal rats.. https://doi.org/10.1159/000014151

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