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PubMed · 10395558

The nuclear pore complex: from molecular architecture to functional dynamics.

D Stoffler·B Fahrenkrog·U Aebi
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Abstract

Toward dissecting the molecular composition and architecture of the nuclear pore complex (NPC), over the past 18 months novel nucleoporins and NPC subcomplexes were identified and characterized. The three-dimensional structure of isolated yeast NPCs was determined by electron cryomicroscopy. New specimen preparation and labeling protocols localized a number of nucleoporins and NPC subcomplexes within the three-dimensional architecture of the yeast NPC. Structural changes of native NPCs mediated by physiological effectors such as calcium or ATP were monitored by time-lapse atomic force microscopy, thus revealing a first glimpse of the NPC's functional dynamics.

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AnimalsHumansMembrane GlycoproteinsNuclear EnvelopeNuclear ProteinsJournal ArticleResearch Support, Non-U.S. Gov'tReview

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D Stoffler, B Fahrenkrog, U Aebi. 1999. The nuclear pore complex: from molecular architecture to functional dynamics.. https://doi.org/10.1016/s0955-0674(99)80055-6

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