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PubMed · 10527905

Visualizing "greengold" clusters in the STEM.

J S Wall
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Abstract

"Greengold" is a large metallic cluster thought to contain 75 gold atoms in a compact 1.4-nm-diameter core surrounded by an organic shell. Scanning transmission electron microscope imaging shows uniform mass and size distributions with an apparent mass of 24 kDa, unaffected by radiation damage. The signal-to-noise ratio is adequate for visualization at low dose and in the presence of a relatively thick biological matrix. Under some conditions these clusters have a slight tendency to form linear chains and 2-D hexagonal arrays with a spacing of 2.6 nm. The parameters presented permit estimation of the feasibility of proposed labeling experiments.

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ColorDose-Response Relationship, RadiationGold CompoundsImage Processing, Computer-AssistedMicroscopy, Electron, Scanning TransmissionOrganometallic CompoundsSignal Processing, Computer-AssistedTobacco Mosaic VirusJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J S Wall. 1999. Visualizing "greengold" clusters in the STEM.. https://doi.org/10.1006/jsbi.1999.4148

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