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PubMed · 10572621

Microprocessor-controlled freezing device for cryopreservation of cell samples.

D Djurić·V Drndarević·D Vujić
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Abstract

A freezing device for cryopreservation of blood mononuclear cells has been developed. The device is microcontroller operated, allowing cell freezing by a fully automatic, unattended process. To ensure optimum preservation, the temperature in the cell suspension uniformly decreases from room temperature to -100 degrees C and then the samples are transferred to long-term storage. The performance of the device has been tested using both physiological solution and a sample of cell suspension. The control of temperature variation of cell suspension in the entire temperature range has been realised with an accuracy better than +/- 0.1%. The viability of cells recovered from the frozen samples was 95%. The nitrogen consumption for one cycle of cryopreservation was 1.51. In addition to the fully automatic mode, the manual and semi-automatic modes are available for research purposes. The device has been designed using low cost and widely used electronic components and materials, it is compact and simple to operate.

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AlgorithmsCell SurvivalCryopreservationEquipment DesignHumansLeukocytes, MononuclearMicrocomputersJournal Article

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D Djurić, V Drndarević, D Vujić. 1999. Microprocessor-controlled freezing device for cryopreservation of cell samples.. https://pubmed.ncbi.nlm.nih.gov/10572621/

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