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PubMed · 10580106

Human napsin A: expression, immunochemical detection, and tissue localization.

V Schauer-Vukasinovic·D Bur·D Kling·F Grüninger·T Giller
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Abstract

A novel aspartic proteinase, called napsin, has recently been found in human and mouse. Due to high similarity with cathepsin D a structural model of human napsin A could be built. Based on this model a potential epitope SFYLNRDPEEPDGGE has been identified, which was used to immunize rabbits. The resulting antibody was employed in monitoring the expression of recombinant human napsin A in HEK293 cell line. Western blot analysis confirmed the specificity of the antibody and showed that human napsin A is expressed as a single chain protein with the molecular weight of approximately 38 kDa. Immunohistochemical studies revealed high expression levels of napsin A in human kidney and lung but low expression in spleen.

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Amino Acid SequenceAntibodiesAspartic Acid EndopeptidasesBlotting, WesternCells, CulturedEpitopesHumansImmunohistochemistryKidneyLungMolecular Sequence DataSequence Homology, Amino AcidTissue DistributionJournal Article

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V Schauer-Vukasinovic, D Bur, D Kling, F Grüninger, T Giller. 1999-11-26. Human napsin A: expression, immunochemical detection, and tissue localization.. https://doi.org/10.1016/s0014-5793(99)01458-1

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