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PubMed · 10620142

Wide-field optically sectioning fluorescence microscopy with laser illumination.

M A Neil·A Squire·R Juskaitis·P I Bastiaens·T Wilson
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Abstract

We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one-dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.

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AnimalsCOS CellsImage EnhancementIsoenzymesLasersMicroscopy, ConfocalMicroscopy, FluorescenceProtein Kinase CProtein Kinase C-alphaJournal ArticleResearch Support, Non-U.S. Gov't

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M A Neil, A Squire, R Juskaitis, P I Bastiaens, T Wilson. 2000. Wide-field optically sectioning fluorescence microscopy with laser illumination.. https://doi.org/10.1046/j.1365-2818.2000.00656.x

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