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A forced-flow membrane reactor for transfructosylation using ceramic membrane.

K Nishizawa·M Nakajima·H Nabetani
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Abstract

A forced-flow membrane reactor system for transfructosylation was investigated using several ceramic membranes having different pore sizes. beta-Fructofuranosidase from Aspergillus niger ATCC 20611 was immobilized chemically to the inner surface of a ceramic membrane activated by a silane-coupling reagent. Sucrose solution was forced through the ceramic membrane by crossflow filtration while transfructosylation took place. The saccharide composition of the product, which was a mixture of fructooligosaccharides (FOS), was a function of the permeate flux, which was easily controlled by pressure. Using 0.2 micrometer pore size of symmetric ceramic membrane, the volumetric productivity obtained was 3.87 kg m(-3) s(-1), which was 560 times higher than that in a reported batch system, with a short residence time of 11 s. The half-life of the immobilized enzyme in the membrane was estimated to be 35 days by a long-term operation.

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Aspergillus nigerBioreactorsBiotechnologyCeramicsEnzymes, ImmobilizedFructoseJournal ArticleResearch Support, Non-U.S. Gov't

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K Nishizawa, M Nakajima, H Nabetani. 2000-04-05. A forced-flow membrane reactor for transfructosylation using ceramic membrane.. https://doi.org/10.1002/(sici)1097-0290(20000405)68%3A1%3C92%3A%3Aaid-bit11%3E3.0.co%3B2-1

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