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PubMed · 10710524

S-nitrosoglutathione-induced decrease in calcium sensitivity of airway smooth muscle.

C M Pabelick·D O Warner·W J Perkins·K A Jones
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Abstract

The purpose of this study was to examine whether the nitric oxide donor S-nitrosoglutathione (GSNO) relaxes canine tracheal smooth muscle (CTSM) strips by decreasing Ca(2+) sensitivity [i.e., the amount of force for a given intracellular Ca(2+) concentration ([Ca(2+)](i))]. We further investigated whether GSNO decreases Ca(2+) sensitivity by altering the relationship between regulatory myosin light chain (rMLC) phosphorylation and [Ca(2+)](i) and the relationship between force and rMLC phosphorylation. GSNO (100 microM) relaxed intact CTSM strips contracted with 45 mM KCl by decreasing Ca(2+) sensitivity in comparison to control strips without significantly decreasing [Ca(2+)](i). GSNO reduced the amount of rMLC phosphorylation for a given [Ca(2+)](i) but did not affect the relationship between isometric force and rMLC phosphorylation. These results show that in CTSM strips contracted with KCl, GSNO decreases Ca(2+) sensitivity by affecting the level of rMLC phosphorylation for a given [Ca(2+)](i), suggesting that myosin light chain kinase is inhibited or that smooth muscle protein phosphatases are activated by GSNO.

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AnimalsCalciumDogsFemaleGlutathioneIn Vitro TechniquesIntracellular MembranesMaleMuscle ContractionMuscle, SmoothMyosin Light ChainsNitric Oxide DonorsNitroso CompoundsOsmolar ConcentrationPhosphorylationS-NitrosoglutathioneTracheaJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

C M Pabelick, D O Warner, W J Perkins, K A Jones. 2000. S-nitrosoglutathione-induced decrease in calcium sensitivity of airway smooth muscle.. https://doi.org/10.1152/ajplung.2000.278.3.l521

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