PubMed · 10797049
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S Moffatt, R Bhopal. 2000-05-06. Study on environmental hazards is flawed.. https://pubmed.ncbi.nlm.nih.gov/10797049/
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In order to understand the role of coordinated ligands in controlling the biotoxicity of chromium (III), interactions of three types of chromium (III) complexes viz. trans-diaquo [1,2 bis (salicyledeneamino) ethane chromium (III) perchlorate, [(Cr(salen)(H(2)O)(2)](ClO(4)); tris (ethylenediamine) chromium (III) chloride, [Cr(en)(3)]Cl(3), and monosodium ethylene diamine tetraacetato monoaquo chromiate (III), [Cr(EDTA)(H(2)O)]Na with BSA has been investigated. Spectroscopic and equilibrium dialysis studies show that the two cationic complexes Cr(salen)(H(2)O)(+)(2) and Cr(en)(3+)(3) bind to the protein with a protein-metal ratio of 1:8 and 1:4. The anionic complex Cr(EDTA)(H(2)O)(-) binds to the protein with a protein-metal ratio of 1:2. The binding constant K(b) as estimated from the fluorescence quenching studies has been found to be 7.6 +/- 0.4 x 10(3) M(-1), 3.1 +/- 0.2 x 10(2) M(-1), and 1.8 +/- 0.2 x 10(2) M(-1) for Cr(salen)(H(2)O)(+)(2), Cr(en)(3+)(3), and Cr(EDTA)(H(2)O)(-) respectively indicating that the thermodynamic stability of protein-chromium complex is Cr(salen)(H(2)O)(+)(2) > Cr(en)(3+)(3) approximately Cr(EDTA)(H(2)O)(-). The complexes Cr(salen)(H(2)O)(+)(2) and Cr(EDTA)(H(2)O)(-) in the presence of hydrogen peroxide have been found to bring about protein degradation, whereas Cr(en)(3+)(3) does not bring about any protein damage. This clearly shows that the nature of the chromium (III) complex plays a major role in the biotoxicity of chromium (III).
The development of a new method for the determination of Cr(III) hydrolytic polymerisation products using capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) is described. The results indicate that CE-ICP-MS can be used to separate and detect monomeric and polymeric Cr(III) species. The various species migrate through the capillary at a rate proportional to their equilibrium distribution, which is dictated by the solution pH, metal ion concentration and ageing period. In general, the data suggest that the relative mobility follows the order trimer>dimer>monomer. The experimentally determined speciation shows a good qualitative agreement with that described in the literature. Independent confirmation of the presence of polymeric Cr(III) species was performed by ionspray mass spectrometry.