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PubMed · 10835627

Computer-based three-dimensional visualization of developmental gene expression.

J Streicher·M A Donat·B Strauss·R Spörle·K Schughart·G B Müller
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Abstract

A broad understanding of the relationship between gene activation, pattern formation and morphogenesis will require adequate tools for three-dimensional and, perhaps four-dimensional, representation and analysis of molecular developmental processes. We present a novel, computer-based method for the 3D visualization of embryonic gene expression and morphological structures from serial sections. The information from these automatically aligned 3D reconstructions exceeds that from single-section and whole-mount visualizations of in situ hybridizations. In addition, these 3D models of gene-expression patterns can become a central component of a future developmental database designed for the collection and presentation of digitized, morphological and gene-expression data. This work is accompanied by a web site (http://www.univie.ac.at/GeneEMAC).

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Anatomy, Cross-SectionalAnimalsAutomationComputer SimulationDatabases, FactualEmbryo, MammalianEmbryonic and Fetal DevelopmentGene Expression ProfilingGene Expression Regulation, DevelopmentalGenetic MarkersImage Processing, Computer-AssistedIn Situ HybridizationInternetMiceMorphogenesisOrgan SpecificityPattern Recognition, AutomatedReproducibility of ResultsSensitivity and SpecificitySoftwareTranscriptional ActivationJournal Article

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J Streicher, M A Donat, B Strauss, R Spörle, K Schughart, G B Müller. 2000. Computer-based three-dimensional visualization of developmental gene expression.. https://doi.org/10.1038/75989

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