A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 1: direct binding.

M D Connolly; S B Park; B M Reedy; R F Standaert

doi:10.1016/s0960-894x(00)00134-7

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A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 1: direct binding.

M D Connolly·S B Park·B M Reedy·R F Standaert

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Abstract

The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competitive binding using NLSs immobilized on the solid phase (TentaGel resin).

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Alkaline Phosphatase Amino Acid Sequence Antigens, Polyomavirus Transforming Binding, Competitive Biotin Cell Nucleus Colorimetry Indicators and Reagents Indoles Molecular Sequence Data Nuclear Proteins Peptides Protein Binding Receptors, Cytoplasmic and Nuclear Streptavidin alpha Karyopherins Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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M D Connolly, S B Park, B M Reedy, R F Standaert. 2000-05-01. A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 1: direct binding.. https://doi.org/10.1016/s0960-894x(00)00134-7

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