A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 1: direct binding.
The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competitive binding using NLSs immobilized on the solid phase (TentaGel resin).
Alkaline Phosphatase↗