A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 2: competitive binding.

S B Park; B M Reedy; R F Standaert

doi:10.1016/s0960-894x(00)00135-9

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A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 2: competitive binding.

S B Park·B M Reedy·R F Standaert

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Abstract

A qualitative assay for the evaluation of soluble ligands of the nuclear import receptor karyopherin alpha has been developed. The assay relies on competition with an immobilized ligand, the nuclear localization signal (NLS) from nucleoplasmin, for binding to the receptor, which is detected by an enzyme-linked colorimetric method.

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Amino Acid Sequence Antigens, Polyomavirus Transforming Binding, Competitive Cell Nucleus Colorimetry Molecular Sequence Data Nuclear Proteins Nucleoplasmins Peptides Phosphoproteins Protein Binding Receptors, Cytoplasmic and Nuclear alpha Karyopherins Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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S B Park, B M Reedy, R F Standaert. 2000-05-01. A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 2: competitive binding.. https://doi.org/10.1016/s0960-894x(00)00135-9

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